Improvement of Paraffin Sections by Immersion of Embedded Tissues in Water

Cutuly, Elizabeth Chalmers; Cutuly, Eugene

doi:10.1126/science.80.2085.564

Cited by 14 publications

(5 citation statements)

References 2 publications

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“…demonstrated that paraffin blocks can be stored in water to decrease brittleness. [ 33 34 ] Baker introduced the use of a mixture of nine volumes of 60% ethanol and one volume of glycerol (Baker's fluid) and found it to be better than water alone. [ 35 ] Carlquist recommended the use of ethylenediamine as a softening agent but it could not be extended for human tissues due to its hazardous nature.…”

Section: Types Of Nail Biopsymentioning

confidence: 99%

Nail biopsy: A user's manual

Grover

Bansal

2018

Indian Dermatol Online J

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Section: Types Of Nail Biopsymentioning

confidence: 99%

Nail biopsy: A user's manual

Grover

Bansal

2018

Indian Dermatol Online J

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“…Turner's paper highlights the use of fabric conditioner in the reconstitution of mummified tissue prior to paraffin-wax sectioning, the rationale being one of rehydration of the mummified remains. Cutuly and Cutuly, 3 in 1934, and later Tahmisian and Slifer,4 in 1942, demonstrated that paraffin wax is penetrated by aqueous solutions and that storing blocks in water obviates brittleness. Baker,5 in 1941, proposed the use of a mixture of nine volumes of 60% ethanol and one volume of glycerol in preference to water alone.…”

Section: Introductionmentioning

confidence: 96%

Use of softening agents to improve the production of formalin-fixed, paraffin-embedded sections of nail tissue: an assessment

Orchard

Torres

Sounthararajah

2008

British Journal of Biomedical Science

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The use of tissue softeners to enhance the quality of tissue sections of heavily keratotic tissue is not widely published. There are very few indicators in the scientific literature that attempt to compare and contrast the benefits and disadvantages of such techniques, as most are passed down through word of mouth rather than through published data. This study attempts to present a preliminary evaluation of several methods employing tissue softeners to facilitate the preparation of reproducible, good-quality formalin-fixed, paraffin-embedded sections of nail tissue. A standard 10-minute surface application of each softener is employed for all paraffin-embedded tissue in order to ensure consistency. The results show that the use of Veet (hair remover), Fairy Liquid or fabric conditioner provides the most beneficial results. Thus, widely available products can be used in preference to specific commercially produced reagents that have no clear benefits and can cost considerably more to purchase. This study will form the basis of a more in-depth evaluation of the most beneficial softeners, in an attempt to determine optimal parameters for their use in routine histopathology laboratories.

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“…The majority of reagents used to soften tissue blocks have arisen from very early work performed in the first half of the last century. [1][2][3][4][5] Many of these substances contained hazardous constituents such as phenol, 5 and this remains a common constituent of commercial histological tissue softening agents.…”

Section: Introductionmentioning

confidence: 99%