Improvement of nutritive perfusion after free tissue transfer by local heat shock-priming-induced preservation of capillary flowmotion

Rücker, Martin; Kadirogullari, Bülent; Vollmar, Brigitte; Spitzer, W.J.; Menger, Michael D.

doi:10.1016/j.jss.2004.07.012

Cited by 8 publications

(6 citation statements)

References 27 publications

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“…A similar effect was described by Ruecker et al [15] in their study on osteomyocutaneous flaps and is likely to be the result of the antioxidative action of biliverdin, which prevents the up-regulation of leukocytic adhesion molecules such as ICAM-1 [28]. …”

Section: Discussionsupporting

confidence: 59%

“…A heat shock leads to the expression of cytoprotective heat shock proteins (HSPs), which belong to a family of proteins that induce immunological cell activities, thermotolerance, buffering of expression of mutations and macrophage-mediated wound healing [11,12]. The upregulation of HSPs, however, induces not only intracellular but also extracellular processes [13-15]. In tissues, stress conditioning can reduce interstitial edema formation and improve perfusion as a result of blood flow upregulation [16].…”

Section: Introductionmentioning

confidence: 99%

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Increase in periosteal angiogenesis through heat shock conditioning

et al. 2011

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ObjectiveIt is widely known that stress conditioning can protect microcirculation and induce the release of vasoactive factors for a period of several hours. Little, however, is known about the long-term effects of stress conditioning on microcirculation, especially on the microcirculation of the periosteum of the calvaria. For this reason, we used intravital fluorescence microscopy to investigate the effects of heat shock priming on the microcirculation of the periosteum over a period of several days.MethodsFifty-two Lewis rats were randomized into eight groups. Six groups underwent heat shock priming of the periosteum of the calvaria at 42.5°C, two of them (n = 8) for 15 minutes, two (n = 8) for 25 minutes and two (n = 8) for 35 minutes. After 24 hours, a periosteal chamber was implanted into the heads of the animals of one of each of the two groups mentioned above. Microcirculation and inflammatory responses were studied repeatedly over a period of 14 days using intravital fluorescence microscopy. The expression of heat shock protein (HSP) 70 was examined by immunohistochemistry in three further groups 24 hours after a 15-minute (n = 5), a 25-minute (n = 5) or a 35-minute (n = 5) heat shock treatment. Two groups that did not undergo priming were used as controls. One control group (n = 8) was investigated by intravital microscopy and the other (n = 5) by immunohistochemistry.ResultsDuring the entire observation period of 14 days, the periosteal chambers revealed physiological microcirculation of the periosteum of the calvaria without perfusion failures. A significant (p < 0.05) and continuous increase in functional capillary density was noted from day 5 to day 14 after 25-minute heat shock priming. Whereas a 15-minute exposure did not lead to an increase in functional capillary density, 35-minute priming caused a significant but reversible perfusion failure in capillaries. Non-perfused capillaries in the 35-minute treatment group were reperfused by day 10. Immunohistochemistry demonstrated an increase in cytoprotective HSP70 expression in the periosteum after a 15-minute and a 35-minute heat shock pretreatment when compared with the control group. The level of HSP70 expression that was measured in the periosteum after 25 minutes of treatment was significantly higher than the levels observed after 15 or 35 minutes of heat shock exposure.ConclusionA few days after heat shock priming over an appropriate period of time, a continuous increase in functional capillary density is seen in the periosteum of the calvaria. This increase in perfusion appears to be the result of the induction of angiogenesis.

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Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Increase in periosteal angiogenesis through heat shock conditioning

et al. 2011

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“…However, further studies will have to look into this. Over expression of HSP following laser irradiation has been well documented in the fields of dermatology, ophthalmology and more recently in cartilage reshaping [13,14,[46][47][48]. In fact, some HSP were identified in bone through immunolocalization in a paper by Tiffee et al [49].…”

Section: Discussionmentioning

confidence: 99%

“…For example, Rucker et al studied the effects of heat shock preconditioning on the transfer of an osteomyocutaneous flap. They observed an improvement in nutritive perfusion in the free tissue transfer associated with the preservation of capillary flow motion [13]. Vascular preconditioning induces a heat shock response in the arterial wall characterized by the preservation of medial cell integrity and a limitation of the proliferative response and intimal accumulation attributable to the second injury [9].…”

Section: Introductionmentioning

confidence: 99%

Laser preconditioning of calvarial bone prior to an X‐ray radiation injury: A preliminary in vivo study of the vascular response

et al. 2008

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Background and Objectives: Thermal preconditioning prior to injury induces a cytoprotective effect on soft tissues and promotes their recovery. Lasers are an adequate tool to generate controlled and reproducible heat. X-ray irradiation induces a chronic antiangiogenic effect on bone, affecting its healing and remodeling processes. The aim of this study was to investigate the effect of laser preconditioning on the re-vascularization of X-ray irradiated bone. Materials and Methods: A bone chamber was implanted onto the calvaria of rabbits to study the vascularization process. Digital pictures were taken of the vascular plexus at the target bone site using a modified digital camera. Vascular density (VD) was determined using image processing. It was defined as the ratio of blood vessel pixels to the total number of pixels to the region of interest. Laser preconditioning was performed with a diode laser (810 nm, 2 W, 3 seconds, 48 J/cm 2 , 4 mm). A 12-week follow-up study was performed on 20 rabbits divided into four groups: #1: control group (n ¼ 5); #2: laser irradiation alone (n ¼ 5). #3: X-ray radiation (18.75 Gy) alone (n ¼ 5), #4: laser preconditioning 24 hours prior to X-ray radiation (n ¼ 5). Results: VD remained stable during the 12-week follow up for group #1. No significant difference was observed between laser irradiation group (#2) and control group (#1) (P > 0.5). The angiolytic action of X-ray radiation was confirmed in groups #3 and #4, which were statistically different from group #1 (P<0.001). However, the decrease of the vascularization was limited in group #4 highlighting a different evolution between group #3 and #4 (P<0.05). These results were confirmed by histological analysis. Discussion and Conclusion: The bone chamber is an effective reproducible method for the longitudinal analysis of the dynamics of vascularization. Our findings have shown that laser preconditioning is capable of preserving vascularization in an X-ray irradiated bone site, thus suggesting a novel approach for promoting the healing of bone tissue in which the vascular supply has been damaged.

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“…Un stress tissulaire contrôlé (qu'il soit d'origine mécanique, thermique ou chimique) avant une exposition à un choc supraphysiologique induit un effet cytoprotecteur sur les tissus mous [1][2][3][4]. Cette mise en condition tissulaire permet aux cellules de résister à une agression métabolique secondaire physiologiquement létale.…”

Section: Introductionunclassified