Improvement of nutritional value and functional properties of soybean glycinin by protein engineering

Kim, Chan-Shick; Kamiya, Seigo; Sato, Toshiro; Utsumi, Shigeru; Kito, Makoto

doi:10.1093/protein/3.8.725

Cited by 73 publications

(51 citation statements)

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“…Among the two different cultivar treatments Bivanij cultivar has the highest (2.35mg/ml) seed protein and the Azad cultivar has the lowest seed protein (2.11mg/ml) and the difference between them was not significant (table2). Similar results were reported by Kim et al, 1990;Suoyi Han et al, 2009. Table1. Analysis of variance for grain protein in chickpea cultivars under drought stress and nitrogen fertilizer starter *and **: Significant at 5% and 1% probability levels, respectively…”

Section: Resultssupporting

confidence: 91%

“…Accumulation of specific proteins and other compounds for nutrient storage to high levels is one of the characteristic events during seed development (Singh et al, 1993). Improvement of storage protein in seed is being given more and more attention all over the world (Kim et al, 1990). Protein is the performer of life activity, the change of plant morphology corresponds with the change of relative proteins (Singh et al, 1993).…”

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Study on Seed Protein and Protein Profile Pattern of Chickpea (Cice arietinum L.) by SDS-PAGE under Drought Stress and Fertilization

Ashrafi¹,

Pourbozorg²,

Kor³

et al. 2015

Int J Life Sci

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Article InformationA field experiment was performed in order to evaluate the effects of drought stress and application of starter nitrogen fertilizer on seed storage proteins and protein pattern in chickpea cultivars. Experiment was performed in split-factorial using randomized complete block design with three replications. The experiment was laid out in a split-factorial design with drought stress in main plots and cultivar with nitrogen fertilizer in subplots with three replications. The experimental treatments consisted of three levels of drought stress [severe drought stress (S2), moderate drought stress (S1) and no drought stress (S0)] and two cultivars of chickpea consist of Azad and Bivanij and 2 N levels. Plants were either not given any N fertilizer (N0), or fertilized by N fertilizer at the rate of 25 kg ha-1 (N25). The results of this study showed that the effects of drought stress on seed storage proteins and protein yield, effect of cultivars on protein yield were significant. With increase of drought stress seed storage proteins was increased and protein yield decreased. Severe drought stress treatment has the highest seed protein and the control treatment has the lowest seed protein. Non stress treatment has the highest protein yield and the severe drought stress treatment has the lowest protein yield. The SDS-PAGE results revealed that no effects treatments on the protein banding patterns but the related severe drought stress bands were chromatic, because they have highest protein concentration in some protein bands.

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Section: Resultssupporting

confidence: 91%

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confidence: 99%

Study on Seed Protein and Protein Profile Pattern of Chickpea (Cice arietinum L.) by SDS-PAGE under Drought Stress and Fertilization

Ashrafi¹,

Pourbozorg²,

Kor³

et al. 2015

Int J Life Sci

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“…Now that the three-dimensional structures have been elucidated completely in the molecules of b-conglycinin and glycinin, it is possible to improve the qualities of soybean storage proteins both physiologically and physicochemically through a genetic modification. For instance, Kim et al (1990) made a modified A 1a B 1b gene which has four additional methionyl residues near the C-termini. This modified A 1a B 1b is excellent in both gelling and emulsifying properties.…”

Section: Quality Improvement Of Soy Food Productsmentioning

confidence: 99%

Recent Progress in Research and Technology on Soybeans

Fukushima

2001

FSTR

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For a long time, it had been considered that soybean storage proteins play only a role as traditional nutrients and other soybean minor constituents such as isoflavones, saponins, trypsin inhibitors, phytic acid, lectin, etc., act as antinutrient factors. At present, however, these substances have all been recognized to have exciting roles in the prevention of heart disease, cancer, osteoporosis, etc. Besides these physiological effects, soybean storage proteins exhibit excellent functional properties physicochemically in food systems, such as gelation, binding, emulsification, fat and water absorption, etc. On the other hand, there are some substances having undesirable properties in soybeans, such as offflavors, allergens, etc. Recently, there was a great progress in the research of a molecular basis on these functionalities, off-flavors, and allergenicities. By applying these results for soybean breeding, the creation of the new cultivars or lines having more improved properties is in progress. Another highlight in soybean research is the success of the crystallization of ␤-conglycinin and glycinin and the subsequent complete determination of their three-dimensional molecular structures through X-ray crystallographic analysis. This paper overviews these recent investgations.

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“…We have previously proposed that the criteria for judging proper conformation should be as follows: (1) the solubility should be comparable with that of globulins, (2) there must be self-assembly into trimers, and (3) there must be high-level expression (=10% of total E. coli proteins). 7,9) Densitometric scanning of the gels showed that the expression levels of normal A1aB1b, A1aB1bM1 and A1aB1bM2 were more than 20% of total E. coli proteins, and 85%, 70% and 60% of expressed normal A1aB1b, A1aB1bM1 and A1aB1bM2 were respectively recovered in the soluble fraction ( Fig. 3-1).…”

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confidence: 98%

Improved Bile Acid-binding Ability of Soybean Glycinin A1a Polypeptide by the Introduction of a Bile Acid-binding Peptide (VAWWMY)

Choi

Adachi

Utsumi

2004

Bioscience, Biotechnology, and Biochemistry

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Improvement of nutritional value and functional properties of soybean glycinin by protein engineering

Cited by 73 publications

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Study on Seed Protein and Protein Profile Pattern of Chickpea (Cice arietinum L.) by SDS-PAGE under Drought Stress and Fertilization

Study on Seed Protein and Protein Profile Pattern of Chickpea (Cice arietinum L.) by SDS-PAGE under Drought Stress and Fertilization

Recent Progress in Research and Technology on Soybeans

Improved Bile Acid-binding Ability of Soybean Glycinin A1a Polypeptide by the Introduction of a Bile Acid-binding Peptide (VAWWMY)

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