Improvement of natamycin production by controlling the morphology of <scp><i>Streptomyces gilvosporeus</i> Z8</scp> with microparticle talc in seed preculture

Yue, Chaoping; Xu, Haitao; Yu, Yingying; Yu, Xin; Yu, Min; Zhang, Chen; You, Qian; Xia, Shaofan; Ding, Zixian; Fu, Hao; Zeng, Xin; Feng, Li

doi:10.1002/jctb.6668

Cited by 6 publications

(2 citation statements)

References 39 publications

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“…Actinomycetes representing the Streptomyces genus are highly relevant in the context of drug manufacturing due to their secondary metabolism encompassing a wide spectrum of biologically active molecules [ 1 ]. It is well known that filamentous morphology is one of the key factors influencing the growth and secondary metabolite production in Streptomyces cultures [ 2 – 5 ]. Therefore, the importance of seeking new methods to control actinomycete morphology in submerged cultivation processes is unquestionable.…”

Section: Introductionmentioning

confidence: 99%

Morphological-metabolic analysis in Streptomyces rimosus microparticle-enhanced cultivations (MPEC)

Ścigaczewska,

Boruta,

Bizukojć

2024

Bioprocess Biosyst Eng

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Streptomyces produce a broad spectrum of biologically active molecules such as oxytetracycline and rimocidin, which are widely used in human and animal treatments. microparticle-enhanced cultivation (MPEC) is one of the tools used for Streptomyces bioprocesses intensification by the control of mycelial morphology. In the present work, morphological changes of Streptomyces rimosus caused by the addition of 10 µm talc microparticles in MPEC were correlated with the biosynthetic activity of the microorganism. Comparing the runs with and without microparticles, major morphological changes were observed in MPEC, including the deformation of pellets, variation of their size, appearance of hyphae and clumps as well as the aggregation of mycelial objects. The presence of talc microparticles also influenced the levels of the studied secondary metabolites produced by S. rimosus. Comparing control and MPEC runs, the addition of talc microparticles increased the amounts of oxytetracycline (9-fold), 2-acetyl-2-decarboxamido-oxytetracycline (7-fold), milbemycin A3+4[O] (3-fold) and CE 108 (1.5-fold), while rimocidin (27-ethyl) and milbemycin β11+4[O] production was reduced. In summary, the addition of talc microparticles to S. rimosus cultivations led to the development of smaller morphological forms like hyphae and clumps as well as to the changes in the amounts of secondary metabolites. Graphical abstract

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Section: Introductionmentioning

confidence: 99%

Morphological-metabolic analysis in Streptomyces rimosus microparticle-enhanced cultivations (MPEC)

Ścigaczewska,

Boruta,

Bizukojć

2024

Bioprocess Biosyst Eng

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“…The physical phenomenon, such as oxygen transfer and shear rate, also influence the morphology of Streptomyces (Ribeiro et al, 2021). Directive and quick-acting approaches, like the addition of microparticles and macroparticles, have also been used to regulate mycelial morphology (Böl et al, 2020; Yue et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Insights into the mechanism of mycelium transformation ofStreptomyces toxytriciniinto pellet

Kumar

Khusboo²,

Rajput

et al. 2023

Preprint

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Formation of the mycelial pellet in submerged cultivation of Streptomycetes is unwanted in industrial fermentation processes as it imposes mass transfer limitations, changes in the rheology of a medium, and affects the production of secondary metabolites. Though detailed information is not available about the factors involved in regulating mycelial morphology but it is studied that culture conditions and genetic information of strain play a key role. Moreover, the proteomic study has revealed the involvement of low molecular weight proteins such as; DivIVA, FilP, ParA, Scy, and SsgA proteins in apical growth and branching of hyphae which results in the establishment of the mycelial network. The present study proposes the mechanism of pellet formation of Streptomyces toxytricini (NRRL B-5426) with the help of microscopic and proteomic analysis. The microscopic analysis revealed that growing hyphae followed a certain organized path of growth and branching, which was further converted into the pellet, and proteomic analysis revealed the production of low molecular weight proteins, which possibly participate in the regulation of pellet morphology.

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Effect of process parameters and microparticle addition on polygalacturonase activity and fungal morphology of Aspergillus sojae

Germeç

Karahalil

Yatmaz

et al. 2021

Biomass Conv. Bioref.

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In this study, the effects of process parameters (initial glucose concentrations, inoculation rates, and nitrogen sources including yeast extract, beef extract, and ammonium nitrate instead of peptone) on polygalacturonase production by Aspergillus sojae in the shaking incubator were examined. Subsequently, the effect of talcum microparticles on activity and morphology was investigated. Based on the results, the highest activity and lowest pellet diameter were 41.91 U/mL and 1411.9 µm when initial glucose concentration and inoculation size were 20 g/L and 12% (v/v), respectively. Besides, as inoculation rate increased, maximum specific growth rate and saturation constant decreased from 0.39 to 0.27 day −1 and 21.26 to 3.50 g/L, respectively. It was determined that highest activity and lowest pellet diameter were found as 18.53 U/mL and 2953.0 µm when medium was supplemented with 2.5 g/L yeast extract. It was also found that as yeast extract and beef extract concentrations increased, pellet diameter decreased. Additionally, medium was supplemented with talcum as a microparticle (0-25 g/L), and maximum activity was 26.59 U/mL (pellet diameter was 2756.3 µm) when talcum concentration was 5 g/L. In addition, as talcum concentration increases from 0.1 to 5 g/L, the biomass concentration increased relatively, but the enzyme activity increased significantly. However, although talcum concentration in the medium is increased until 20 g/L, while the biomass concentration increased, the activity decreased. Compared to control fermentation (without talcum), the activity increased ninefold. Polygalacturonase was also partially purified via ultrafiltration with the purification fold of 1.84. Consequently, fungal morphology in submerged fermentation can be controlled by microparticle addition fermentation, and thus the enzyme activity can be increased.

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Improvement of natamycin production by controlling the morphology of Streptomyces gilvosporeus Z8 with microparticle talc in seed preculture

Cited by 6 publications

References 39 publications

Morphological-metabolic analysis in Streptomyces rimosus microparticle-enhanced cultivations (MPEC)

Morphological-metabolic analysis in Streptomyces rimosus microparticle-enhanced cultivations (MPEC)

Insights into the mechanism of mycelium transformation ofStreptomyces toxytriciniinto pellet

Effect of process parameters and microparticle addition on polygalacturonase activity and fungal morphology of Aspergillus sojae

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