Improvement of Microsurgical Techniques in Orthotopic Rat Liver Transplantation

Ariyakhagorn, Veeravorn; Schmitz, Volker; Olschewski, Peter; Polenz, Dietrich; Boas-Knoop, Sabine; Neumann, Ulf; Puhl, Gero

doi:10.1016/j.jss.2008.04.003

Cited by 19 publications

(16 citation statements)

References 22 publications

(23 reference statements)

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“…Hypothermic perfusion of the liver is carried out through the portal vein. The operation is completed with a sectioning of the common bile duct hepatic artery, the portal vein, the IH-IVC and the suprahepatic-inferior vena cava (SH-IVC), which is carried out at the intrathoracic level, respecting a diaphragmatic flap around it [60,61,63] .…”

Section: Donor Operationmentioning

confidence: 99%

“…The end-to-end anastomosis of the IH-IVC is also performed using the cuff technique. Next, the recipient IH-IVC is unclamped [60][61][62][63] . Lastly, the cannula of the donor common bile duct is introduced into the lumen of the recipient common bile duct, thus performing an end-to-end cholecocholedochostomy, over which the greater omentum is placed to avoid fistulas [60,61] .…”

Section: Ivc Pvmentioning

confidence: 99%

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A half century (1961-2011) of applying microsurgery to experimental liver research

Aller

Arias²,

Prieto³

et al. 2012

WJH

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The development of microsurgery has been dependent on experimental animals. Microsurgery could be a very valuable technique to improve experimental models of liver diseases. Microdissection and microsutures are the two main microsurgical techniques that can be considered for classifying the experimental models developed for liver research in the rat. Partial portal vein ligation, extrahepatic cholestasis and hepatectomies are all models based on microdissection. On the other hand, in portacaval shunts, orthotopic liver transplantation and partial heterotopic liver transplantation, the microsuture techniques stand out. By reducing surgical complications, these microsurgical techniques allow for improving the resulting experimental models. If good experimental models for liver research are successfully developed, the results obtained from their study might be particularly useful in patients with liver disease. Therefore experimental liver microsurgery could be an invaluable way to translate laboratory data on liver research into new clinical diagnostic and therapeutic strategies.

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Section: Donor Operationmentioning

confidence: 99%

Section: Ivc Pvmentioning

confidence: 99%

A half century (1961-2011) of applying microsurgery to experimental liver research

Aller

Arias²,

Prieto³

et al. 2012

WJH

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“…Organ and cell recipients were female Lewis rats (n = 26, 180-200 g BW) without prior treatment. The liver transplantation was carried out with sutured vena cava and portal vein anastomoses and the cuff technique for the hepatic artery and bile duct [Lee et al, 1973;Steffen et al, 1989;Ariyakhagorn et al, 2009;Matevossian et al, 2009]. All liver transplants were performed by one experienced surgeon (D.P.).…”

Section: Animalsmentioning

confidence: 99%

Allogeneic Liver Transplantation and Subsequent Syngeneic Hepatocyte Transplantation in a Rat Model: Proof of Concept for in vivo Tissue Engineering

Rohn¹,

Schroeder²,

Riedel³

et al. 2016

Cells Tissues Organs

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Objectives: Stable long-term functioning of liver cells after transplantation in humans is still not achieved successfully. A new approach for successful engraftment of liver cells may be the transplantation of syngeneic cells into an allogeneic liver graft. We therefore developed a new rat model for combined liver and liver cell transplantation (cLCTx) under stable immunosuppression. Materials and Methods: After inducing a mitotic block, liver grafts from female donor rats (Dark Agouti) were transplanted into female recipients (Lewis). In male Lewis rats, liver cell proliferation was induced with subsequent cell isolation and transplantation into female recipients after organ transplantation. Y-chromosome detection of the transplanted male cells was performed by quantitative polymerase chain reaction (qPCR) and fluorescence in situ hybridization (FisH) with localization of transplanted cells by immunohistochemistry. Results: Immunohistochemistry demonstrated the engraftment of transplanted cells, as confirmed by FisH, showing repopulation of the liver graft with 15.6% male cells (± 1.8 SEM) at day 90. qPCR revealed 14.15% (± 5.09 SEM) male DNA at day 90. Conclusion: Engraftment of transplanted syngeneic cells after cLCTx was achieved for up to 90 days under immunosuppression. Immunohistochemistry indicated cell proliferation, and the FisH results were partly confirmed by qPCR. This new protocol in rats appears feasible for addressing long-term functioning and eventually the induction of operational tolerance in the future.

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“…The intra-operative complications of PV included vein tearing and torsion. One-week survival was also noted for each group as an assessment of the operation [5,9].…”

Section: Assessmentsmentioning

confidence: 99%

“…However, at the same time, liver transplantation in rats is one of the most difficult animal transplant models due to complicated nature of the operation, numerous required procedures, and existence of an anhepatic phase [5,6]. The anhepatic phase duration is a key determinant of a successful operation.…”

Section: Introductionmentioning

confidence: 99%

A Technique of Recipient Portal Venoplasty and Cuff Insertion for Portal Revascularization in Orthotopic Rat Liver Transplantation

Cai

et al. 2012

Journal of Surgical Research

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Improvement of Microsurgical Techniques in Orthotopic Rat Liver Transplantation

Cited by 19 publications

References 22 publications

A half century (1961-2011) of applying microsurgery to experimental liver research

A half century (1961-2011) of applying microsurgery to experimental liver research

Allogeneic Liver Transplantation and Subsequent Syngeneic Hepatocyte Transplantation in a Rat Model: Proof of Concept for in vivo Tissue Engineering

A Technique of Recipient Portal Venoplasty and Cuff Insertion for Portal Revascularization in Orthotopic Rat Liver Transplantation

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