Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel

Mohammadi, Najmeh Sabahi; Khiabani, Mahmoud Sowti; Ghanbarzadeh, Babak; Mokarram, Reza Rezaei

doi:10.1016/j.jbiotec.2020.07.002

Cited by 32 publications

(17 citation statements)

References 74 publications

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“…Meanwhile, the amide I peak appearing at 1658 cm −1 is associated with the CO stretching vibration, and the amide II peak appearing at 1541 is associated with the N−H bending and C−N stretching vibrations. 30 N 2 adsorption isotherm showed the synthesized UIO-66 and UIO-66/Pro were mesoporous materials (Figure 2C,D). Brunauer−Emmett−Teller (BET) surface areas and micropore volumes of UIO-66 and UIO-66/Pro were 650.41 and 372.49 m 2 /g and 0.001738 and 0.075 cm 3 /g, respectively.…”

Section: Resultsmentioning

confidence: 99%

Proline-Modified UIO-66 as Nanocarriers to Enhance Candida rugosa Lipase Catalytic Activity and Stability for Electrochemical Detection of Nitrofen

Cheng

Lai

Tan

et al. 2021

ACS Appl. Mater. Interfaces

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Immobilization can be used to improve the stability of lipases and enhances lipase recovery and reusability, which increases its commercial value and industrial applications. Nevertheless, immobilization frequently causes conformational changes of the lipases, which decrease lipase catalytic activity. in the present work, we synthesized UIO-66 and grafted UIO-66 crystals with proline for immobilization of Candida rugosa lipase (CRL). As indicated by steady-state fluorescence microscopy, grafting of proline onto UIO-66 crystals induced beneficial conformational change in CRL.

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Section: Resultsmentioning

confidence: 99%

Proline-Modified UIO-66 as Nanocarriers to Enhance Candida rugosa Lipase Catalytic Activity and Stability for Electrochemical Detection of Nitrofen

Cheng

Lai

Tan

et al. 2021

ACS Appl. Mater. Interfaces

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“…Figure S3 illustrates the FTIR spectra of SSA and the hydrogels (NAH0, NAH5, and NASH2.5). From the SSA spectra, the absorption band appeared at 3424 cm −1 was due to the SiOH of silanol groups, 36 and three absorption bands at 1106, 813, and 470 cm −1 could be respectively contributed to asymmetric stretching vibration, symmetric stretching vibration, and bending vibration of SiOSi 36 . As for the hydrogels, the broad bands at 3200–3500 cm −1 could be assigned to OH and NH groups 11 .…”

Section: Resultsmentioning

confidence: 95%

“…This was ascribed to that the SSA or AA was added to the hydrogels to increase the content of crosslinker or monomer. Thus, it increased the degree of crosslinking density of hydrogels and led to a higher reticulated network 19,36,37 . However, with excessive SSA addition, such as NASH4.5, it can be seen that its pore structure was collapsed and tangled, implying that it was not suitable for the convenience of water absorption.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of dual pH‐ and temperature‐sensitive poly(N‐isopropylacrylamide‐co‐acrylic acid)/sewage sludge ash hydrogel with the simultaneously high performance of swelling and deswelling

Pan

Huang

Guo

et al. 2021

Polymers for Advanced Techs

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An advanced dual pH‐ and temperature‐sensitive hydrogel (NASH2.5) was optimally synthesized through modification of N‐isopropylacrylamide (NIPAM) hydrogel with introducing 5 mol% acrylic acid (AA) and 2.5 wt% sewage sludge ash (SSA). The swelling kinetic results showed that NASH2.5 exhibited both high equilibrium swelling ratio and swelling rate, which was attributed to the higher porous structure as shown in scanning electron microscope, and the more hydrogen bonding formed inside of the hydrogel as investigated in Fourier transform infrared spectrometer. In addition, its curve was better fitted to the pseudo‐second‐order model, indicating that the water absorption process was dominated by chemisorption through forming the hydrogen bonding among the water molecules and carboxyl/silanol groups of the hydrogel. Compared with the pure NIPAM hydrogel, the water transport mechanism switched from Case I diffusion to Case II diffusion by introduction of AA and further SSA. Furthermore, through the results of the deswelling kinetics in pH value change (from 9 to 4 and 2, respectively), temperature value change (from 25 to 40, 50, and 60°C, respectively), and dual pH and temperature values changes, NASH2.5 not only presented a high pH sensitivity, but also showed high sensitive to temperature by achieving high water recovery ratio in rapid dehydrated rate. Therefore, the dual stimuli‐sensitive hydrogel with the simultaneously high performance of swelling and deswelling would provide a suitable alternative for specific applications such as pollutant adsorption.

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“…Adsorption immobilization is a physical method that results from van der Waals and other noncovalent interactions, including hydrophobic interactions and hydrogen bonding electrostatic linkages among the support and the attached enzyme. 76 Adsorption immobilization method is a naive, inexpensive, and reversible technique of enzyme immobilization. The adsorbed enzymes are usually resistant to proteolysis and aggregation because of their hydrophobic interaction with interfaces.…”

Section: Enzyme Immobilization Methods In Hydrogelmentioning

confidence: 99%

“…and eco-friendly, it is widely used in the preparation of hydrogels. 75 , 76 The internal network of polyvinyl alcohol hydrogel has free water, crystalline and swollen amorphous PVA domains; it creates a porous structure and can be effective for various applications, including enzyme immobilization. 77 , 78 As shown in Table 2 , the various polymer based hydrogels was used in this field.…”

Section: Hydrogel Matrixes As Enzyme Carriersmentioning

confidence: 99%

Recent Advances of Macromolecular Hydrogels for Enzyme Immobilization in the Food Products

et al. 2021

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Enzymes are one of the main biocatalysts with various applications in the food industry. Stabilization of enzymes on insoluble carriers is important due to the low reuse, low operational stability, and high cost in applications. The immobility and the type of carrier affect the activity of the immobile enzyme. Hydrogels are three-dimensionally cross-linked macromolecular network structures designed from various polymers. Hydrogels can provide a matrix for an immobile enzyme due to their extraordinary properties such as high water absorbing capacity, carrier of bioactive substances and enzymes, biocompatibility, safety, and biodegradability. Therefore, this study mainly focuses on some enzymes (Lactase, Lipases, Amylases, Pectinase, Protease, Glucose oxidase) that are of special importance in the food industry. These enzymes could be immobilized in the hydrogels constructed of macromolecules such as kappa-carrageenan, chitosan, arabic gum, pectin, alginate, and cellulose. At last, in the preparation of these hydrogels, different enzyme immobilization methods in macromolecular hydrogels, and effect of hydrogels on enzyme activity were discussed.

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Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel

Cited by 32 publications

References 74 publications

Proline-Modified UIO-66 as Nanocarriers to Enhance Candida rugosa Lipase Catalytic Activity and Stability for Electrochemical Detection of Nitrofen

Proline-Modified UIO-66 as Nanocarriers to Enhance Candida rugosa Lipase Catalytic Activity and Stability for Electrochemical Detection of Nitrofen

Synthesis of dual pH‐ and temperature‐sensitive poly(N‐isopropylacrylamide‐co‐acrylic acid)/sewage sludge ash hydrogel with the simultaneously high performance of swelling and deswelling

Recent Advances of Macromolecular Hydrogels for Enzyme Immobilization in the Food Products

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