Improvement of isolated microspore culture of wheat (Triticum aestivum L.) through ovary co-culture

Hu, Tianci; Kasha, K. J.

doi:10.1007/s002990050271

Cited by 62 publications

(46 citation statements)

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“…Co-culture with ovaries has been tested and found to be a useful method to increase green plant production in hexaploid wheat microspore culture (Puolimatka et al 1996;Hu and Kasha 1997;Zheng et al 2002). It has also been used for recalcitrant genotypes in barley (Devaux and Li 2001) and shown to be beneficial in durum wheat (J'Aiti et al 1999;Cistué et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Improvements in the production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture

Cistué

Romagosa

Batlle³

et al. 2009

Plant Cell Rep

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Section: Introductionmentioning

confidence: 99%

Improvements in the production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture

Cistué

Romagosa

Batlle³

et al. 2009

Plant Cell Rep

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“…One possible solution to reduce culture stress could be to refresh the medium and add fresh ovaries to each Petri dish at day 21 in induction culture, as suggested by earlier reports [13]- [15]. However, the refreshing protocol does not address the competition for space or possibly oxygen, which leads to a third and better solution: the combination of refreshing and transfer of multicellular structures and pre-embryoids to a separate Petri dish.…”

Section: Discussionmentioning

confidence: 99%

Developmental Dynamics of Wheat (<i>Triticum aestivum</i> L.) Microspores under Culture

Zheng¹,

Bieren²,

Griggs³

2015

ABB

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Doubled haploid production via microspore culture is a technique known to accelerate crop breeding by shortening the breeding cycle through achieving homozygosity in one generation. Prior research observed that some embryogenic microspores aborted their development before reaching the embryoid stage. Such embryogenic abortion reduces embryoid yield, making microspore cultures less efficient. The present research aims at identifying stages during which microspore development is susceptible to embryogenic abortion. Information gained through delineation of the developmental dynamics of microspores in culture could be used to improve the efficiency of microspore culture. Embryogenic microspores were isolated from stress-treated wheat (Triticum aestivum L.) tillers and cultured in liquid medium. The development of embryogenic microspores was monitored over a 35 day period. At day 7, 10, 14, 21, 28, and 35, the developing microspores were counted and categorized into multicellular structures, pre-embryoids, immature embryoids and mature embryoids. The results showed that 44% -62% of embryogenic microspores halted their development before the mature embryoid stage. Of these aborted embryogenic microspores, 21% -33% terminated as multicellular structures, 16% -19% arrested their development as pre-embryoids, and 7% -10% halted development as immature embryoids. Identifying factors that are responsible for embryogenic abortion and finding remedy to the issue will help improve the efficiency of doubled haploid production.

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“…Sugar starvation by placing anthers on a medium with mannitol as the carbohydrate source is one of the most commonly used stress pre-treatments (Caredda et al, 2000;Kasha et al, 2001; Cistué et Soriano et al, 2007;Castillo et al, 2015). This pre-treatment has resulted in consistently high chromosome doubling rates in barley (Kasha et al, 2001;Shim et al, 2006) and wheat (Hu and Kasha, 1997). In addition, Cistué et al (2006) reported that pre-treating the anthers in 0.7 M mannitol for five days improved green plant regeneration in durum wheat.…”

Section: Discussionmentioning

confidence: 99%

Effect of D Genome on Wheat Anther Culture Response After Cold and Mannitol Pretreatment

Lazaridou¹,

Pankou

Xynias³

et al. 2016

Acta Biologica Cracoviensia S. Botanica

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The present study was conducted to determine the effect of the D genome on embryoid induction and green plant regeneration in wheat anther culture and how it is influenced by low temperature and mannitol treatment. For this reason, the anther culture response of two Canadian bread wheat cultivars and their extracted tetraploids (AABB) was studied. As controls two cultivars well responding to anther-culture (i.e. cvs. Kavkaz/Cgn and Acheron) and a no-responding cultivar (cv. Vergina) were used. Approximately 3000 anthers of these cultivars were cultured and three pre-treatments were applied: cold pre-treatment for 7 and 18 days at 4 o C, and 0.3M mannitol for seven days at 4 o C. W14 and 190-2 were used as induction and regeneration media, respectively, and the basic MS medium as the rooting medium. No green plants were produced from the tetraploids, which supports the view that the D-genome chromosomes are necessary for androgenic response in wheat. Furthermore, the Canadian cultivars performed better after 18-day pre-treatment at 4 o C. The extracted tetraploids produced fewer embryoids and performed better after seven days of cold pre-treatment. The controls well responding to anther culture performed better than the Canadian cultivars, although their best response was recorded after seven-day cold pre-treatment. Cultivar Vergina produced no green plants. The presence of mannitol influenced negatively both embryoid and green plant production. It was concluded that the D genome plays a crucial role in anther culture response of wheat and that this response is influenced by both the genotype and the duration of cold pre-treatment.

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Improvement of isolated microspore culture of wheat (Triticum aestivum L.) through ovary co-culture

Cited by 62 publications

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Improvements in the production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture

Improvements in the production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture

Developmental Dynamics of Wheat (<i>Triticum aestivum</i> L.) Microspores under Culture

Effect of D Genome on Wheat Anther Culture Response After Cold and Mannitol Pretreatment

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Improvement of isolated microspore culture of wheat (Triticum aestivum L.) through ovary co-culture

Cited by 62 publications

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Improvements in the production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture

Improvements in the production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture

Developmental Dynamics of Wheat (&lt;i&gt;Triticum aestivum&lt;/i&gt; L.) Microspores under Culture

Effect of D Genome on Wheat Anther Culture Response After Cold and Mannitol Pretreatment

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Developmental Dynamics of Wheat (<i>Triticum aestivum</i> L.) Microspores under Culture