Improvement of islet culture with sericin

Ogawa, A.; Terada, Satoshi; Kanayama, Takanori; Miki, Masao; Morikawa, Mitsuhiro; Kimura, Toshihisa; Yamaguchi, Akio; Sasaki, Michihito; Yamada, Hideyuki

doi:10.1016/s1389-1723(04)00270-1

Cited by 44 publications

(15 citation statements)

References 7 publications

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“…This finding is consistent with the results reported by Ogawa (2000) that Cmedium is applicable to a wider range of cultivars including Indica, Japonica and Javanica subspecies. The induction rate of type-I calli from all the five Javanica rice cultivars on C-modified medium was higher than that on MS2 medium, especially for Rojolele.…”

supporting

confidence: 93%

Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars

Rachmawati

Anzai

2006

Plant Biotechnology

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The purpose of this study was to develop a reproducible efficient procedure for the transformation of Javanica rice cultivars from Indonesia. Five rice cultivars cultivated in Indonesia now were examined for their capacity on the callus growth, plant regeneration and transformation. Their potential was affected by genotype and medium. Regarding the quality of callus, type I calli produced higher plant regeneration frequency than type II calli. So type I calli were inoculated with A. tumefaciens harboring binary plasmid pAFT14, which had a hygromycin resistance (hpt) gene and a gus gene. In this study, we examined two media for two steps in the tissue culture process for transformation, i.e. callus inducing and plant regeneration. The results show that C-modified medium was the suitable media for callus induction in the most Javanica rice cultivars. Agrobacterium-mediated transformation system has been extended to five Javanica rice cultivars. Among them, Rojolele consistently gave the best performance.Key words: Callus, Javanica rice, transformation.Plant Biotechnology 23, 521-524 (2006) This article can be found at http://www.jspcmb.jp/ Tissue Culture Note tolerant to drought (gogo rice). The callus induction rate was calculated after 4 weeks of culture as the percentage of the seeds producing callus.As results, significant difference was found in callus induction rate and quality of callus induced among the five genotypes of Javanica rice (Table 2). Although a considerable variation was observed, all genotypes of rice can induce callus on both media. Two types of calli, embryogenic and non-embryogenic ones, were obtained from the embryo scutellum. The embryogenic calli (type-I) were relatively dry, milky white in color, compact and nodular in appearance ( Figure 1A, B). In contrast, non-embryogenic calli (type-II) were soft and watery, mostly white but some brown in color ( Figure 1C, D). Four weeks after induction, the calli were subcultured onto fresh medium, which had the same composition as that used for callus induction. Following the subculture, the type-I calli grew about 5 to 10-fold in volume 30 days after transfer. However, the type-II calli turned brown and died.In general, callus induction rate of the five Javanica rice cultivars on C-modified medium was higher than that on MS2 medium (Table 2). This finding is consistent with the results reported by Ogawa (2000) that Cmedium is applicable to a wider range of cultivars including Indica, Japonica and Javanica subspecies. The induction rate of type-I calli from all the five Javanica rice cultivars on C-modified medium was higher than that on MS2 medium, especially for Rojolele. On Cmodified medium, the induction rate of Rojolele was 10 times higher than one on MS2. On the other hand, the induction rate of type-II calli of the five cultivars on MS2 medium was almost the same as that on C-modified medium (Table 2). To determine the regeneration ability, the initiated calli were transferred to a regeneration medium which was composed of ma...

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supporting

confidence: 93%

Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars

Rachmawati

Anzai

2006

Plant Biotechnology

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“…Ogawa et al [83] tested the effect of sericin and FBS on islet cell survival and insulin production and, compared to Morikawa et al [123], on the 12th day, all islets were morphologically intact in FBS culture, while only 50% were intact in sericin culture. On insulin production, sericin was effective in maintenance, but its effects were inferior to FBS.…”

Section: Sericin Properties and Biomedical Applicationsmentioning

confidence: 99%

Silkworm Sericin: Properties and Biomedical Applications

Kunz

Brancalhão

Ribeiro

et al. 2016

BioMed Research International

315

261

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Silk sericin is a natural polymer produced by silkworm, Bombyx mori, which surrounds and keeps together two fibroin filaments in silk thread used in the cocoon. The recovery and reuse of sericin usually discarded by the textile industry not only minimizes environmental issues but also has a high scientific and commercial value. The physicochemical properties of the molecule are responsible for numerous applications in biomedicine and are influenced by the extraction method and silkworm lineage, which can lead to variations in molecular weight and amino acid concentration of sericin. The presence of highly hydrophobic amino acids and its antioxidant potential make it possible for sericin to be applied in the food and cosmetic industry. The moisturizing power allows indications as a therapeutic agent for wound healing, stimulating cell proliferation, protection against ultraviolet radiation, and formulating creams and shampoos. The antioxidant activity associated with low digestibility of sericin that expands the application in the medical field, such as antitumour, antimicrobial and anti-inflammatory agent, anticoagulant, acts in colon health, improving constipation and protects the body from obesity through improved plasma lipid profile. In addition, the properties of sericin allow its application as a culture medium and cryopreservation, in tissue engineering and for drug delivery, demonstrating its effective use, as an important biomaterial.

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“…In this study, four callus induction media, N6D medium, 7,8) C medium, 20) MSB medium, 12) and MS2 medium, 13) were examined to obtain embryogenic calli of Rojolele. Callus growth was measured four weeks after culture.…”

Section: Resultsmentioning

confidence: 99%

“…2). The Cmodified medium was derived from the C medium 20) by increasing the maltose concentration to 15 g/l, and the pH of medium increased from 4.5 to 5.8. We also examined another Javanica rice variety, Bulu, using the C modified medium for callus induction.…”

Section: Resultsmentioning

confidence: 99%