Improvement of Insulin Sensitivity after Peroxisome Proliferator-Activated Receptor-α Agonist Treatment Is Accompanied by Paradoxical Increase of Circulating Resistin Levels

Haluzı́k, Martin; Lacinová, Z; Dolinkova, Marketa; Haluzíková, D; Housa, Daniel; Hořı́nek, A.; Vernerová, Zdeňka; Kumstyrova, T.

doi:10.1210/en.2005-1624

Cited by 62 publications

(37 citation statements)

References 44 publications

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“…The key distinction between our protocol and that of others is that we use an arterial catheter for obtaining blood samples. This is in contrast to the more widely used approach of obtaining blood samples by severing the tip of the tail 4,7,11,12,[14][15][16][17] . The advantage of sampling from an arterial catheter is that the experiment is conducted in a conscious and unrestrained mouse.…”

Section: Discussionmentioning

confidence: 93%

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice

Ayala

Bracy

Malabanan

et al. 2011

JoVE

103

101

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Type 2 diabetes is characterized by a defect in insulin action. The hyperinsulinemic-euglycemic clamp, or insulin clamp, is widely considered the "gold standard" method for assessing insulin action in vivo. During an insulin clamp, hyperinsulinemia is achieved by a constant insulin infusion. Euglycemia is maintained via a concomitant glucose infusion at a variable rate. This variable glucose infusion rate (GIR) is determined by measuring blood glucose at brief intervals throughout the experiment and adjusting the GIR accordingly. The GIR is indicative of whole-body insulin action, as mice with enhanced insulin action require a greater GIR. The insulin clamp can incorporate administration of isotopic 2[ 14 C]deoxyglucose to assess tissue-specific glucose uptake and [3-3 H]glucose to assess the ability of insulin to suppress the rate of endogenous glucose appearance (endoRa), a marker of hepatic glucose production, and to stimulate the rate of whole-body glucose disappearance (Rd).

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Section: Discussionmentioning

confidence: 93%

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice

Ayala

Bracy

Malabanan

et al. 2011

JoVE

103

101

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“…Rossi et al reported that shifting the source of fat to fish oil for 2 months increased plasma levels of both leptin and adiponectin in rats 25) . Haluzik et al reported that fenofibrate treatment did not increase serum adiponectin in mice fed a carbohydrate diet 26) . This is also consistent with our previous observation using C57BL/6J mice that plasma adiponectin increased significantly in fish oil groups, but not in the fish oil with fenofibrate group.…”

Section: Discussionmentioning

confidence: 99%

Anti-Obesity Effect of Fish Oil and Fish Oil-Fenofibrate Combination in Female KK Mice

Arai

Kim

Chiba

et al. 2009

JAT

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Aim:The aim of our study is to elucidate the effects of EPA-or DHA-rich fish oil, and of the latter plus fenofibrate, on lipid metabolism in female KK mice. Methods: Female KK mice were fed purified experimental diets containing lard/safflower oil (4:6, Lard/SO), EPA-rich fish oil (EPA), DHA-rich fish oil (DHA), or DHA-rich fish oil plus 0.2% (w/w) fenofibrate (DHA FF) for 8 weeks. At the end of the experiments, we measured levels of plasma lipids, hepatic triglycerides, and cholesterol, as well as the hepatic mRNA expression of lipogenic and lipidolytic genes. Results: The final body weight of EPA-and DHA-fed groups was significantly lower than that of the Lard/SO-fed group, and that of the DHA FF-fed group was the lowest. All three fish oil treatments significantly reduced plasma insulin levels. Hepatic lipid levels significantly decreased in all three of these groups compared with the Lard/SO-fed group. Plasma adiponectin increased in both the EPAand DHA-fed groups, but the increase was suppressed in the DHA FF-fed group. Hepatocytes of Lard/SO-fed mice were filled with numerous fat droplets, but fat accumulation was inhibited in both EPA-and DHA-fed mice and was significantly prevented by fenofibrate treatment. SREBP-1c mRNA levels were decreased by about half in EPA-and DHA-fed mice compared with Lard/SO-fed mice. FAS, Insig-1, HMG-CoA reductase, and LDL-receptor mRNA levels also markedly decreased in both EPA-and DHA-fed mice, but there was no additional decrease in DHA FF fed mice. Fenofibrate treatment significantly induced mRNA expression of AOX and UCP-2, but not of PPAR . Conclusion: These data suggest that fish oil inhibited body weight gain and exhibited an anti-obesity effect through the inhibition of lipid synthesis in female KK mice. Furthermore, fenofibrate treatment markedly inhibited body weight gain by the induction of fatty acid oxidation. Plasma adiponectin levels did not increase in mice fed DHA-rich fish oil with fenofibrate, although white adipose tissue (WAT) weight significantly decreased. We considered that adiponectin sensitivity increased more in mice fed DHA-rich fish oil with fenofibrate than in mice fed DHA-rich fish oil alone.

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“…Blood glucose concentrations were monitored at 10-min intervals throughout the clamp studies. Basal and clamp hepatic glucose production (HGP), glucose disposal rate (GDR), and glucose infusion rate (GIR) were calculated as described previously [13].…”

Section: Observation and Assessmentmentioning

confidence: 99%

Effects of autologous adipose-derived stem cell infusion on type 2 diabetic rats

Chen

et al. 2015

Endocr J

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Type 2 diabeTes melliTus (T2DM) accounts for more than 90% of all diabetic patients and is characterized by insulin resistance, hyperglycemia, systemic low-grade inflammation, and relative lack of insulin. Most T2DM patients experience β-cell exhaustion several years after diagnosis, and thus have to accept insulin therapy. However, insulin therapy is inconvenient for patients and does not completely prevent the development of diabetic complications. Stem cells are an attractive option to ameliorate diabetes for its abundant source and potential to acquire glucose-dependent insulin secretory function. Adult stem cells are an especially good candidate for its safety in terms of tumorigenicity and ethical concerns.Adipose-derived stem cells (ASC) have been stud- The effects and possible mechanisms of adipose-derived stem cells (ASC) infusion on type 2 diabetic rats were investigated in this study. Twenty normal male Sprague-Dawley rats were included in normal control group, and 40 male diabetic rats were randomly divided into diabetic control group and ASC group (which received ASC infusion). After therapy, levels of fasting plasma glucose (FPG), HbA1c, serum insulin and C-peptide, recovery of islet cells, inflammatory cytokines, and insulin sensitivity were analyzed. After ASC infusion, compared with diabetic control group, hyperglycemia in ASC group was ameliorated in 2 weeks and maintained for about 6 weeks, and plasma concentrations of insulin and C-peptide were significantly improved (P<0.01). Number of islet β cells and concentration of vWF in islets in ASC group increased, while activity of caspase-3 in islets was reduced. Moreover, concentrations of TNF-α, IL-6 and IL-1β in ASC group obviously decreased (P<0.05). The expression of GLUT4, INSR, and phosphorylation of insulin signaling molecules in insulin target tissues were effectively improved. ASC infusion could aid in T2DM through recovery of islet β cells and improvement of insulin sensitivity. Autologous ASC infusion might be an effective method for T2DM.

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Improvement of Insulin Sensitivity after Peroxisome Proliferator-Activated Receptor-α Agonist Treatment Is Accompanied by Paradoxical Increase of Circulating Resistin Levels

Cited by 62 publications

References 44 publications

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice

Anti-Obesity Effect of Fish Oil and Fish Oil-Fenofibrate Combination in Female KK Mice

Effects of autologous adipose-derived stem cell infusion on type 2 diabetic rats

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