Improvement of Hydroxyapatite Deposition on Titanium Dental Implant Using ArF Laser Ablation: Effect on Osteoblast Biocompatibility In Vitro

Hashimoto, Yoshiya; Kusünoki, Masanobu; Hatanaka, Ryota; Hamano, Keiko; Nishikawa, Hiroaki; Hosoi, Yoshihiko; Hontsu, Shigeki; Nakamura, Masaaki

doi:10.4028/3-908158-05-2.282

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“…This can explain why the Ti-Mg and Ti-MgHA surface in the present study showeda higher proliferation level with respect to the Ti-S surface. Sampaio et al 9 and Hashimoto et al 10 showed osteoblasts grow faster on high-crystallinity HA coatings than uncoated Ti surface. The calcium-phosphatelayers on the Ti substrate also enhance cell attachment and proliferation.…”

Section: Discussionmentioning

confidence: 99%

Effect of magnesium and calcium phosphate coatings on osteoblastic responses to the titanium surface

et al. 2013

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PURPOSEThe aim of this study was to evaluate the surface properties and in vitro bioactivity to osteoblasts of magnesium and magnesium-hydroxyapatite coated titanium.MATERIALS AND METHODSThemagnesium (Mg) and magnesium-hydroxyapatite (Mg-HA) coatings on titanium (Ti) substrates were prepared by radio frequency (RF) and direct current (DC) magnetron sputtering.The samples were divided into non-coated smooth Ti (Ti-S group), Mg coatinggroup (Ti-Mg group), and Mg-HA coating group (Ti-MgHA group).The surface properties were evaluated using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). The surface roughness was evaluated by atomic force microscopy (AFM). Cell adhesion, cell proliferation and alkaline phosphatase (ALP) activity were evaluated using MC3T3-E1 cells. Reverse transcription polymerase chain reaction (RT-PCR) analysis was performed.RESULTSCross-sectional SEM images showed that Mg and Mg-HA depositionson titanium substrates were performed successfully. The surface roughness appeared to be similaramong the three groups. Ti-MgHA and Ti-Mg group had improved cellular responses with regard to the proliferation, alkaline phosphatase (ALP) activity, and bone-associated markers, such as bone sialoprotein (BSP) and osteocalcin (OCN) mRNA compared to those of Ti-S group. However, the differences between Ti-Mg group and Ti-MgHA group were not significant, in spite of the tendency of higher proliferation, ALP activity and BSP expression in Ti-MgHA group.CONCLUSIONMg and Mg-HAcoatings could stimulate the differentiation into osteoblastic MC3T3-E1 cells, potentially contributing to rapid osseointegration.

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Section: Discussionmentioning

confidence: 99%