Improvement of growth performance, immunity and disease resistance in Nile tilapia, Oreochromis niloticus, by using dietary probiotics supplementation

Abstract

This study evaluated the effect of probiotics on the growth performance, some immunological, biochemical and hematological parameters of Nile tilapia. Eight weeks feeding trials were conducted to examine the effect of three types of probiotics, multi-strain probiotics (6×10 7 cfu/g), Bacillus subtilis (1×10 11 cfu/g) and Saccharomyces cerevisiae (2.6×10 10 cfu/g) in commercial names Protexin, Biogen-S and Diamond V respectively. Nile tilapia (24±0.02 g) was fed probiotic-incorporated diets as well as control diet (free of probiotics) in triplicates. At 4th and 8th week of feeding trial, the growth performance of fish fed different probiotics was higher than those fed the control diet. No significant difference was observed among fish fed different probiotics for 4 or 8 weeks. Blood profile showed an increase in hemoglobin (Hb), red blood cell (RBCs), packed cell volume (PCV), total protein, and globulin while aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels decreased in fish fed different types of probiotics. Probiotics supplementation improved fish innate immunity based on lysozyme and respiratory burst activities over the control diet evaluated at 4th and 8th week. Likewise, significant up-regulation of the expression of cytokine genes, interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF α) estimated at 8th week by using quantitative RT-PCR were significantly higher than the control. After experimental period (8 weeks) fish from each group were challenged by pathogenic Aeromonas hydrophila (4×10 8 cfu/ml) which exhibited better relative survival percentages than the controls. Dietary supplementation of probiotics improves growth performance, immune status and disease resistance in Nile tilapia. Also, the best results related to growth obtained from groups fed multi-strain probiotic.

Phytochemical Constituents of Ulva Lactuca and Supplementation to Improve The Nile Tilapia (Oreochromis Niloticus) Haemato-Biochemical Status

“…Serum ALT levels decreased significantly with the first diet. Consistent with our results, [34] used three types of probiotics, multi-strain probiotics (6 × 107 cfu/g), Saccharomyces cerevisiae (2.6 × 1010 cfu/g) and Bacillus subtilis (1 × 1011 cfu/g) and in the diet of Nile tilapia, and showed that ALT and AST levels were reduced in fish fed with different probiotic species. In addition [37] reported the addition of a Nutrasexylam © enzyme supplement containing a mixture of α-amylase (40,000 /g) and βxylanase (6,300u/g) had no adverse effect on fish health and that the concentrations of AST and -ALT in plasma was normal.…”

“…This match with those of [33] who showed that protease enzyme addition increased total protein levels, globulin, and albumin in the serum of Nile tilapia fed diets with high incorporation levels of cotton seed meal and supplemented with exogenous protease. Similarly, [34] used three types of probiotics, multi-strain probiotic (6 × 107 cfu / g), Bacillus subtilis (1 × 1011 cfu / g), and Saccharomyces cerevisiae (2.6 × 1010 cfu / g) in Nile tilapia diet and showed an increase in total protein and globulin in fish fed different probiotic species.…”

Effect of temperature on the growth performance, haematological properties and histomorphology of gill, intestine and liver tissues in juvenile butter catfish Ompok bimaculatus

“…Blood parameters are an important stressor indicator and a health determining factor in aquatic animals (Cho et al., 2015; Dowidar et al., 2018). The temperature has been considered as a major factor impacting the haematology and immunology of finfish (De et al., 2019; Lulijwa et al., 2021).…”

Optimization of 17α-methyltestosterone dose to produce quality mono-sex Nile tilapia Oreochromis niloticus

“…The world's total tilapia production reached approximately 6 million tons in 2020 ( Guenard, 2020 ). The advantageous features of tilapia most importantly faster growth, extreme tolerance capacity to hostile environments, high disease resistivity along with high survival rate make it a predominant culture species ( Alam et al., 2014 ; Dowidar et al., 2018 ; Hossain et al., 2022 ). However, the main obstacle in tilapia culture is its early sexual maturity and frequent spawning in the culture system ( Lind et al., 2015 ).…”