Improvement of growth, fruit weight and early blight disease protection of tomato plants by rhizosphere bacteria is correlated with their beneficial traits and induced biosynthesis of antioxidant peroxidase and polyphenol oxidase

Babu, Anupama Narendra; Jogaiah, Sudisha; Ito, Shin; Nagaraj, Amruthesh Kestur; Tran, Lam‐Son Phan

doi:10.1016/j.plantsci.2014.11.006

Cited by 183 publications

(76 citation statements)

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“…Induction of resistance in host plants is known to be accompanied by an increase in defense-related enzymes, including PPO, PAL, and POX (Reignault et al, 2001; Nandeeshkumar et al, 2008; Jogaiah et al, 2013; Babu et al, 2015). Therefore, we have tested the ability of seed-priming with trehalose in inducing PPO, PAL, and POX using the experimental design described in detail in “Materials and Methods.” The NIC and RC samples showed a significant difference ( P ≤ 0.05) from each other in terms of PPO activities.…”

Section: Resultsmentioning

confidence: 99%

Exogenous Trehalose Treatment Enhances the Activities of Defense-Related Enzymes and Triggers Resistance against Downy Mildew Disease of Pearl Millet

et al. 2016

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In recent years, diverse physiological functions of various sugars are the subject of investigations. Their roles in signal transduction in plant responses to adverse biotic and abiotic stress conditions have become apparent, and growing scientific evidence has indicated that disaccharides like sucrose and trehalose mediate plant defense responses in similar way as those induced by elicitors against the pathogens. Trehalose is a well-known metabolic osmoregulator, stress-protectant and non-reducing disaccharide existing in a variety of organisms, including fungi, bacteria, and plants. Commercially procured trehalose was applied to seeds of susceptible pearl millet (Pennisetum glaucum) cultivar “HB3,” and tested for its ability to reduce downy mildew disease incidence by induction of resistance. Seed treatment with trehalose at 200 mM for 9 h recorded 70.25% downy mildew disease protection, followed by those with 100 and 50 mM trehalose which offered 64.35 and 52.55% defense, respectively, under greenhouse conditions. Furthermore, under field conditions treatment with 200 mM trehalose for 9 h recorded 67.25% downy mildew disease protection, and reduced the disease severity to 32.75% when compared with untreated control which displayed 90% of disease severity. Trehalose did not affect either sporangial formation or zoospore release from sporangia, indicating that the reduction in disease incidence was not due to direct inhibition but rather through induction of resistance responses in the host. Additionally, trehalose was shown to enhance the levels of polyphenol oxidase, phenylalanine ammonia lyase, and peroxidase, which are known as markers of both biotic and abiotic stress responses. Our study shows that osmoregulators like trehalose could be used to protect plants against pathogen attacks by seed treatment, thus offering dual benefits of biotic and abiotic stress tolerance.

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Section: Resultsmentioning

confidence: 99%

Exogenous Trehalose Treatment Enhances the Activities of Defense-Related Enzymes and Triggers Resistance against Downy Mildew Disease of Pearl Millet

et al. 2016

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“…The seeds of Chinese cabbages were sterilized with 2 mL of 0.2% NaClO for 5 min and were then rinsed in 70% ethanol for 30 s. Subsequently, the seeds were washed five times with sterile water (Narendra et al, 2015). The sterilized seeds were soaked in 8.3 lg CFU/mL YL6-GFP suspensions for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…One is the release of organic acids by PSB to dissolve insoluble P, including malic, lactic, acetic, citric, and succinic acids (RodríGuez and Fraga, 1999). The second mechanism is the secretion of enzymes by PSB that are able to degrade insoluble P. Some PSB can even produce plant growth regulators, or phytohormones, such as indole acetic acid (IAA) and gibberellin (GA) (Seo and Park, 2009; Babu et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of a Phosphorus-Solubilizing Bacterium from Rhizosphere Soils and Its Colonization of Chinese Cabbage (Brassica campestris ssp. chinensis)

Wang

et al. 2017

Front. Microbiol.

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Phosphate-solubilizing bacteria (PSB) can promote the dissolution of insoluble phosphorus (P) in soil, enhancing the availability of soluble P. Thus, their application can reduce the consumption of fertilizer and aid in sustainable agricultural development. From the rhizosphere of Chinese cabbage plants grown in Yangling, we isolated a strain of PSB (YL6) with a strong ability to dissolve P and showed that this strain promoted the growth of these plants under field conditions. However, systematic research on the colonization of bacteria in the plant rhizosphere remains deficient. Thus, to further study the effects of PSB on plant growth, in this study, green fluorescent protein (GFP) was used to study the colonization of YL6 on Chinese cabbage roots. GFP expression had little effect on the ability of YL6 to grow and solubilize P. In addition, the GFP-expressing strain stably colonized the Chinese cabbage rhizosphere (the number of colonizing bacteria in the rhizosphere soil was 4.9 lg CFU/g). Using fluorescence microscopy, we observed a high abundance of YL6-GFP bacteria at the Chinese cabbage root cap and meristematic zone, as well as in the root hairs and hypocotyl epidermal cells. High quantities of GFP-expressing bacteria were recovered from Chinese cabbage plants during different planting periods for further observation, indicating that YL6-GFP had the ability to endogenously colonize the plants. This study has laid a solid and significant foundation for further research on how PSB affects the physiological processes in Chinese cabbage to promote plant growth.

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“…PGPR are commonly known as rhizobacteria that inhabit the plant rhizosphere and facilitate plant growth through various mechanisms (Narendra Babu et al . ). For example, Pseudomonas putida H‐2‐3 produces gibberellins, and modulates the hormonal and stress physiology of soybean to improve plant growth under saline and drought conditions (Kang et al .…”

Section: Introductionmentioning

confidence: 97%

Co‐inoculation ofBacillussp. andPseudomonas putidaat different development stages acts as a biostimulant to promote growth, yield and nutrient uptake of tomato

Pantigoso

et al. 2019

J Appl Microbiol

102

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Aims This study builds upon the premise that roots culture distinct bacteria at specific stages of plant growth to benefit of specific microbial services needed at that particular growth stage. Accordingly, we hypothesized that the co‐inoculation of beneficial microbes with distinct properties at specific stages of plant development would enhance plant performance. Methods and Results The chosen microbes were Bacillus pumilus, Bacillus amyloliquefaciens, Bacillus mojavensis and Pseudomonas putida. These microbes were selected based on their specific services ranging from nutrient solubilization, root growth promotion and disease resistance, and were applied to the roots of tomato plants at specific time points when those services were needed the most by the plant. Laboratory and greenhouse studies were conducted to evaluate the effects of co‐inoculation at specific stages of development compared to single microbial applications. Conclusion In general, the combination of three microbes gave the highest biomass and yield without the presence of disease. Applications of three microbes showed the highest root/shoot ratio, and applications of four microbes the lowest ratio. Pseudomonas putida significantly increased fruit macronutrient and micronutrient contents. Significance and Impact of the Study Our studies suggest that co‐inoculation of three or four microbes is a good strategy for healthy crop production.

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Improvement of growth, fruit weight and early blight disease protection of tomato plants by rhizosphere bacteria is correlated with their beneficial traits and induced biosynthesis of antioxidant peroxidase and polyphenol oxidase

Cited by 183 publications

References 50 publications

Exogenous Trehalose Treatment Enhances the Activities of Defense-Related Enzymes and Triggers Resistance against Downy Mildew Disease of Pearl Millet

Exogenous Trehalose Treatment Enhances the Activities of Defense-Related Enzymes and Triggers Resistance against Downy Mildew Disease of Pearl Millet

Isolation and Characterization of a Phosphorus-Solubilizing Bacterium from Rhizosphere Soils and Its Colonization of Chinese Cabbage (Brassica campestris ssp. chinensis)

Co‐inoculation ofBacillussp. andPseudomonas putidaat different development stages acts as a biostimulant to promote growth, yield and nutrient uptake of tomato

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