Improvement of growth and bacoside production in Bacopa monnieri through induced autotetraploidy with colchicine

Abstract

Bacopa monnieri is a medicinal herb that is increasing in demand in Thailand. However, the lack of high-bacoside cultivars has limited pharmaceutical utilization and production. Here, chromosome doubling in B. monnieri was attempt to improve biomass and bacoside content in its seedling. Nodal segments were treated with colchicine (0, 0.025, 0.05, 0.075, 0.1, and 0.5% w/v) for 24 or 48 h before transferring to multiple shoot induction medium (1/2 MS medium supplemented with 0.2 mg L−1 BAP). Of 326 tested clones, 18 and 84 were mixoploids and autotetraploids, respectively. The highest autotetraploid-induction percentage (14.6%) was found after treated with 0.5% (w/v) colchicine, and 48 hours exposure. From 28 selected autotetraploid clones, 21 and 13 have significantly higher fresh and dry weight compared to the diploid clone, respectively. The maximum fresh and dry weight of autotetraploid plants was 2.8 and 2.0-time higher than diploid plants, respectively. Moreover, the maximum total bacoside content (1.55 mg plant−1) was obtained from an autotetraploid plant, which was 2.3-fold higher than the level in diploid plants. These novel autotetraploids have the potential to be developed as resources for value-added improvements in the medicinal and pharmaceutical industries.

In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment

“…3A – 3C ). This characteristic is in line with several other induced polyploid species, such as A nthurium andraeanum ( Chen et al, 2011 ); Lobularia maritima ( Huang et al, 2015 ); Lycium rhuthenicum ( Rao et al, 2019 ); Manihot esculenta ( Zhou, Zeng & Yan, 2017 ); Bacopa monnieri ( Inthima & Sujipuli, 2019 ); Plumbago auriculata ( Jiang et al, 2020 ) and Solanum lycopersicum ( De Alencar, Azevedo & Latado, 2020 ). Detailed histological study on S. lycopersicum leaf blade revealed that thicker leaf in the induced polyploid was the result of greater thickness in the epidermis (adaxial and abaxial) and the parenchyma (spongy and palisade) layers ( De Alencar, Azevedo & Latado, 2020 ).…”

Peer Review #2 of "In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment (v0.1)"

“…This study observed that the octoploid and mixoploid plants of N. cadamba produced thicker leaf than the tetraploid plants (Table 3 and Figures 3A-3C). This characteristic is in line with several Manuscript to be reviewed other induced polyploid species, such as Anthurium andraeanum (Chen et al, 2011); Lobularia maritima (Huang et al, 2015); Lycium rhuthenicum (Rao et al, 2019); Manihot esculenta (Zhou et al, 2017); Bacopa monnieri (Inthima & Sujipuli, 2019); Plumbago auriculata (Jiang et al, 2020) and Solanum lycopersicum (de Alencar et al, 2020). Detailed histological study on S. lycopersicum leaf blade revealed that thicker leaf in the induced polyploid was the result of greater thickness in the epidermis (adaxial and abaxial) and the parenchyma (spongy and palisade) layers (de Alencar et al, 2020).…”

Fingerprinting Profile of Saponin Glycosides in Bacopa Monnieri (L.) Wettst

“…Field-grown Bacopa monnieri showed seasonal, plant part, and growth stage variations in morphology and phytochemicals analyses, which may be related to changes in environmental conditions. 2 A low amount of genetic diversity in plant germplasm gathered from various parts of India has been found in previous studies; this may be due to the plant species' dependence on vegetative growth. 3 Bacosides A and B are said to be present in many products produced from B. monnieri that may be found on the international market.…”