Improvement of Glyphosate Resistance through Concurrent Mutations in Three Amino Acids of the Pantoea sp. 5-Enolpyruvylshikimate-3-  Phosphate Synthase

Liu, Feng; Cao, Yanhui

doi:10.4014/jmb.1801.01026

Cited by 2 publications

(1 citation statement)

References 28 publications

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“…Previously it has been shown that a mutation in EPSPS coding sequence can provide resistance to glyphosate, as demonstrated in goosegrass, Eleusine indica and Pantoea sp. [35,43,44].…”

Section: Discussionmentioning

confidence: 99%

Identification of Structural Variants in Two Novel Genomes of Maize Inbred Lines Possibly Related to Glyphosate Tolerance

Mahmoud

Gracz-Bernaciak

Żywicki

et al. 2020

Plants

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To study genetic variations between genomes of plants that are naturally tolerant and sensitive to glyphosate, we used two Zea mays L. lines traditionally bred in Poland. To overcome the complexity of the maize genome, two sequencing technologies were employed: Illumina and Single Molecule Real-Time (SMRT) PacBio. Eleven thousand structural variants, 4 million SNPs and approximately 800 thousand indels differentiating the two genomes were identified. Detailed analyses allowed to identify 20 variations within the EPSPS gene, but all of them were predicted to have moderate or unknown effects on gene expression. Other genes of the shikimate pathway encoding bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase and chorismate synthase were altered by variants predicted to have a high impact on gene expression. Additionally, high-impact variants located within the genes involved in the active transport of glyphosate through the cell membrane encoding phosphate transporters as well as multidrug and toxic compound extrusion have been identified.

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“…Previously it has been shown that a mutation in EPSPS coding sequence can provide resistance to glyphosate, as demonstrated in goosegrass, Eleusine indica and Pantoea sp. [35,43,44].…”

Section: Discussionmentioning

confidence: 99%

Identification of Structural Variants in Two Novel Genomes of Maize Inbred Lines Possibly Related to Glyphosate Tolerance

Mahmoud

Gracz-Bernaciak

Żywicki

et al. 2020

Plants

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Current Status of Herbicide-Tolerant Protein Research and Strategy for Development of Herbicide-Tolerant Tomato Using CRISPR-Cas9 System

Kim¹,

Park²,

Yang³

et al. 2019

Trends Agric. Life Sci.

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The development of herbicide-tolerant plants provided a convenient farming method. The five most popular herbicides are glyphosate, sulfonylureas/imidazolinones, glufosinate, norflurasone and oxyfluorfen. These herbicides inhibit 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), acetohydroxyacid synthase (AHAS), glutamine synthetase (GS), Phytoene desaturase (PDS) and protoporphyrinogen oxidase (PPO), respectively. Inhibition of EPSPS and AHAS reduces the biosynthesis of phenolic and branched amino acids, respectively, and inhibition of GS and PDS enhances the production of reactive oxygen species and induces plant necrosis. Inhibition of PPO decreases chlorophyll biosynthesis and inhibits plant photosynthesis. These herbicides induce plant death by interacting with their target proteins therefore the development of herbicide resistant plants is based on the discovery of mutant proteins insensitive to these herbicides. We reviewed the development of herbicide-tolerant plants and derived target amino acids for the production of herbicide-tolerant proteins using the CRISPR/Cas9 system in tomatoes.

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Improvement of Glyphosate Resistance through Concurrent Mutations in Three Amino Acids of the Pantoea sp. 5-Enolpyruvylshikimate-3- Phosphate Synthase

Cited by 2 publications

References 28 publications

Identification of Structural Variants in Two Novel Genomes of Maize Inbred Lines Possibly Related to Glyphosate Tolerance

Identification of Structural Variants in Two Novel Genomes of Maize Inbred Lines Possibly Related to Glyphosate Tolerance

Current Status of Herbicide-Tolerant Protein Research and Strategy for Development of Herbicide-Tolerant Tomato Using CRISPR-Cas9 System

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