Improvement of ethanol production by ethanol-tolerant Saccharomyces cerevisiae UVNR56

Thammasittirong, Sutticha Na-Ranong; Thirasaktana, Thanawan; Thammasittirong, Anon; Srisodsuk, Malee

doi:10.1186/2193-1801-2-583

Cited by 45 publications

(20 citation statements)

References 18 publications

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“…The production rate is in agreement with the previous studies [35, 36]. In a recent study, Thammasittirong et al reported 8.6% ( v/v ) production for wild-type strain [37]. …”

Section: Resultssupporting

confidence: 91%

“…Like the palm wine yeast, TBY1 and TGY2 isolates exhibited remarkably high ethanol tolerance comparable to industrial yeasts such as sake and distiller’s yeasts associated with high level of ethanol tolerance [51, 52]. The level of ethanol tolerance of 15% ( v/v ) by the yeast strain UVNR56 [37] is in agreement with previous studies [53, 54]. In our present study, we have seen the similar result for Pa and Or isolates that tolerated up to 12% ethanol, which is much more consistent with the other results ( Tables 5 and 6).…”

Section: Discussionmentioning

confidence: 99%

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Isolation of Saccharomyces cerevisiae from pineapple and orange and study of metal’s effectiveness on ethanol production

Nasir

Rahman

Hossain

et al. 2017

EuJMI

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In view of the anticipated shortage of the traditional supplies of fossil fuels, there is a great deal of interest in the production of ethanol as an alternative biofuel in recent years. The main objective of this research work was to isolate and characterize stress tolerant, high potential ethanol producing yeast strains from various fruit peel. Two yeast isolates from pineapple (Pa) and orange (Or) have been isolated, characterized on the basis of morphological and physic-chemical characters and optimized on ethanol producing capability using sugarcane molasses as substrate. Ethanol production percentage was estimated by Conway method. Isolates were thermotolerant, pH tolerant, ethanol tolerant as well as osmotolerant. They were resistant to Chloramphenicol (30 μg/disc) and Nalidixic acid (30 μg/disc). The isolates showed no killer toxin activity against E. coli. The highest production capacity of the yeasts was found to be 7.39% and 5.02% for Pa and Or, respectively, at pH 5.0, 30 °C temperature in media with an initial reducing sugar concentration of 6.5% for Pa and 5.5% for Or (shaking). Addition of metal ions increased the rate of ethanol production highest to 10.61% by KH2PO4. This study revealed that indigenous yeast isolates could be used to benefit the fuel demand and industrial alcohol industries.

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“…The production rate is in agreement with the previous studies [35, 36]. In a recent study, Thammasittirong et al reported 8.6% ( v/v ) production for wild-type strain [37]. …”

Section: Resultssupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Isolation of Saccharomyces cerevisiae from pineapple and orange and study of metal’s effectiveness on ethanol production

Nasir

Rahman

Hossain

et al. 2017

EuJMI

View full text Add to dashboard Cite

show abstract

“…While day 6 colony-forming units at a 20 wt % solids loading to SSF dropped by about one order of magnitude, colony-forming units at solids loadings of 21.5 wt % and 23 wt % dropped by about three orders of magnitude. The decreased viability can be related with loss of membrane integrity as ethanol titers exceeded 80 g•L −1 due to hyperpolarization of phospholipid cell membranes and resulting greater fluidity and permeability (35). These results are in agreement with the fedbatch glucose fermentations, in that the highest ethanol yields were achieved at 20 wt % solids, where the glucose release was balanced with glucose consumption and ethanol toxicity to maintain greater cell viability.…”

Section: Resultssupporting

confidence: 81%

Overcoming factors limiting high-solids fermentation of lignocellulosic biomass to ethanol

Nguyen

Cai

Kumar

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

139

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Simultaneous saccharification and fermentation (SSF) of solid biomass can reduce the complexity and improve the economics of lignocellulosic ethanol production by consolidating process steps and reducing end-product inhibition of enzymes compared with separate hydrolysis and fermentation (SHF). However, a long-standing limitation of SSF has been too low ethanol yields at the high-solids loading of biomass needed during fermentation to realize sufficiently high ethanol titers favorable for more economical ethanol recovery. Here, we illustrate how competing factors that limit ethanol yields during high-solids fermentations are overcome by integrating newly developed cosolvent-enhanced lignocellulosic fractionation (CELF) pretreatment with SSF. First, fed-batch glucose fermentations by DA revealed that this strain, which has been favored for SSF, can produce ethanol at titers of up to 86 g⋅L Then, optimizing SSF of CELF-pretreated corn stover achieved unprecedented ethanol titers of 79.2, 81.3, and 85.6 g⋅L in batch shake flask, corresponding to ethanol yields of 90.5%, 86.1%, and 80.8% at solids loadings of 20.0 wt %, 21.5 wt %, and 23.0 wt %, respectively. Ethanol yields remained at over 90% despite reducing enzyme loading to only 10 mg protein⋅g glucan [∼6.5 filter paper units (FPU)], revealing that the enduring factors limiting further ethanol production were reduced cell viability and glucose uptake by DA and not loss of enzyme activity or mixing issues, thereby demonstrating an SSF-based process that was limited by a strain's metabolic capabilities and tolerance to ethanol.

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“…The highest amount of bioethanol production was 7% (v/v) in aerobic conditions and 8% (v/v) in aerobic-anaerobic conditions after 72 h. Therefore, the aerobic-anaerobic conditions are suitable for bioethanol production, because biomass was propagated in aerobic conditions and anaerobic conditions are suitable for alcoholic fermentation and ethanol production. Thammasittirong et al produced 8.6% (v/v) ethanol in a medium containing molasses with 28% Brix by S. cerevisiae NR1 [24].…”

Section: Discussionmentioning

confidence: 99%

Optimization of an Industrial Medium from Molasses for Bioethanol Production Using the Taguchi Statistical Experimental-Design Method

Darvishi

Moghaddami

2019

Fermentation

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The production of bioethanol as a clean liquid fuel in a cost-effective way is highly desired by global energetics. Sugar beet molasses is a renewable and cheap substrate for the production of biotechnological products. Therefore, the aim of the current study was the optimization of an industrial medium from molasses for bioethanol production using the Taguchi statistical experimental-design method. First, the growth rate of yeast cells and the amount of ethanol produced by the Saccharomyces cerevisiae strain sahand 101 were investigated in aerobic and aerobic–anaerobic conditions. The yeast strain produced 8% (v/v) bioethanol in a medium containing molasses with 18% Brix in aerobic–anaerobic conditions. The main factors of the medium, including molasses, ammonium sulfate, urea, and pH, were optimized for the increase of bioethanol production by the Taguchi method. Bioethanol production reached 10% (v/v) after optimization of the medium in flask culture. The yeast strain produced 11% (v/v) bioethanol in the bioreactor culture containing the optimized medium, which is an acceptable amount of bioethanol produced from molasses at the industrial scale. The results showed that the Taguchi method is an effective method for the design of experiments aiming to optimize the medium for bioethanol production by reducing the number of experiments and time.

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Improvement of ethanol production by ethanol-tolerant Saccharomyces cerevisiae UVNR56

Cited by 45 publications

References 18 publications

Isolation of Saccharomyces cerevisiae from pineapple and orange and study of metal’s effectiveness on ethanol production

Isolation of Saccharomyces cerevisiae from pineapple and orange and study of metal’s effectiveness on ethanol production

Overcoming factors limiting high-solids fermentation of lignocellulosic biomass to ethanol

Optimization of an Industrial Medium from Molasses for Bioethanol Production Using the Taguchi Statistical Experimental-Design Method

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