Improvement of electrospun polymer fiber meshes pore size by femtosecond laser irradiation

Rebollar, Esther; Cordero, Diego; Martins, Albino; Chiussi, S.; Reis, Rui L.; Neves, Nuno M.; León, B.

doi:10.1016/j.apsusc.2010.12.002

Cited by 27 publications

(27 citation statements)

References 31 publications

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“…They found no significant changes in chemical state after femtosecond laser ablation. Rebollar et al 40 used FTIR and time-of-flight-secondary ion mass spectroscopy (ToF-SIMS) to study the chemical modification of PCL nanofiber scaffolds after femtosecond laser irradiation and also found no significant change in chemical composition.…”

Section: Discussionmentioning

confidence: 99%

Vascular Wall Engineering Via Femtosecond Laser Ablation: Scaffolds with Self-Containing Smooth Muscle Cell Populations

et al. 2011

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For tissue-engineered vascular grafts to reach their full potential, three-dimensional (3D) cellular micro-integration will be necessary. In this study, we utilize femtosecond laser ablation to produce microchannels inside electrospun polycaprolactone (PCL) scaffolds. These microchannels potentially provide spatially controlled cell distributions approaching those observed in vivo. The ability of such laser-ablated microchannels to direct cell seeding was evaluated. The dimensions chosen were 100 μm wide, 100 μm deep and 10 mm long. Femtosecond laser ablation successfully produced these microchannels in the scaffolds without substantially altering the ~900 nm diameter fibers. Flow within these microchannels was studied by injecting fluorescent polystyrene bead solutions. Direct measurement of bead motion yielded an inlet velocity of 2.78 cm s(-1). This was used for modeling two-dimensional (2D) flow using computational fluid dynamics to estimate flow profiles within the microchannel. Successful demonstrations of bead flow were followed by seeding of 500,000 human coronary artery smooth muscle cells (HCASMCs) in proliferative medium at a rate of ~500 μL min(-1). Confocal microscopy and scanning electron microscopy confirmed that the HCASMCs were seeded down the full 10-mm length of the microchannel and stayed within its boundaries. Both nuclei and F-actin were observed within the seeded cells. The presence of F-actin filaments shows that the cells were adhered strongly to the scaffold and remained viable throughout the culture. The concept of "vascular wall engineering" producing intricate cell seeding through microchannels produced via femtosecond laser ablation was validated.

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Section: Discussionmentioning

confidence: 99%

Vascular Wall Engineering Via Femtosecond Laser Ablation: Scaffolds with Self-Containing Smooth Muscle Cell Populations

et al. 2011

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“…Rebollar et al () exploited femtosecond laser processing to drill sufficiently large micropores in electrospun mesh. With tight control over processing conditions, i.e.…”

Section: Enhancement Of Cellular Infiltration In Electrospun Scaffoldsmentioning

confidence: 99%

“…In addition, femtosecond laser ablation can create desired microscale topographical features with precision and increase both pore size and bulk porosity of an electrospun nanofibrous scaffold (Choi et al, 2007). Rebollar et al (2011) exploited femtosecond laser processing to drill sufficiently large micropores in electrospun mesh. With tight control over processing conditions, i.e.…”

Section: Applying Powermentioning

confidence: 99%

A review of key challenges of electrospun scaffolds for tissue-engineering applications

Khorshidi

Solouk

Mirzadeh

et al. 2015

J Tissue Eng Regen Med

428

337

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Tissue engineering holds great promise to develop functional constructs resembling the structural organization of native tissues to improve or replace biological functions, with the ultimate goal of avoiding organ transplantation. In tissue engineering, cells are often seeded into artificial structures capable of supporting three-dimensional (3D) tissue formation. An optimal scaffold for tissue-engineering applications should mimic the mechanical and functional properties of the extracellular matrix (ECM) of those tissues to be regenerated. Amongst the various scaffolding techniques, electrospinning is an outstanding one which is capable of producing non-woven fibrous structures with dimensional constituents similar to those of ECM fibres. In recent years, electrospinning has gained widespread interest as a potential tissue-engineering scaffolding technique and has been discussed in detail in many studies. So why this review? Apart from their clear advantages and extensive use, electrospun scaffolds encounter some practical limitations, such as scarce cell infiltration and inadequate mechanical strength for load-bearing applications. A number of solutions have been offered by different research groups to overcome the above-mentioned limitations. In this review, we provide an overview of the limitations of electrospinning as a tissue-engineered scaffolding technique, with emphasis on possible resolutions of those issues. Copyright © 2015 John Wiley & Sons, Ltd.

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“…3 In particular, laser-induced morphological changes, such as the formation of ripples, 4,5 bubbles, 6 and conical structures, 7 have been extensively studied.…”

Section: Introductionmentioning

confidence: 99%

Assessment of femtosecond laser induced periodic surface structures on polymer films

Rebollar

Aldana

Martín-Fabiani

et al. 2013

Phys. Chem. Chem. Phys.

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aIn this work we present the formation of laser induced periodic surface structures (LIPSS) on spin-coated thin films of several model aromatic polymers including poly(ethylene terephthalate), poly(trimethylene terephthalate) and poly carbonate bis-phenol A upon irradiation with femtosecond pulses of 795 and 265 nm at fluences well below the ablation threshold. LIPSS are formed with period lengths similar to the laser wavelength and parallel to the direction of the laser polarization vector. Formation of LIPSS upon IR irradiation at 795 nm, a wavelength at which the polymers absorb weakly, contrasts with the absence of LIPSS in this spectral range upon irradiation with nanosecond pulses. Real and reciprocal space characterization of LIPSS obtained by Atomic Force Microscopy (AFM) and Grazing Incidence Small Angle X-ray Scattering (GISAXS), respectively, yields well correlated morphological information. Comparison of experimental and simulated GISAXS patterns suggests that LIPSS can be suitably described considering a quasi-one-dimensional paracrystalline lattice and that irradiation parameters have an influence on the order of such a lattice. Fluorescence measurements, after laser irradiation, provide indirect information about dynamics and structure of the polymer at the molecular level. Our results indicate that the LIPSS are formed by interference of the incident and surface scattered waves. As a result of this process, heating of the polymer surface above its glass transition temperature takes place enabling LIPSS formation.

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Improvement of electrospun polymer fiber meshes pore size by femtosecond laser irradiation

Cited by 27 publications

References 31 publications

Vascular Wall Engineering Via Femtosecond Laser Ablation: Scaffolds with Self-Containing Smooth Muscle Cell Populations

Vascular Wall Engineering Via Femtosecond Laser Ablation: Scaffolds with Self-Containing Smooth Muscle Cell Populations

A review of key challenges of electrospun scaffolds for tissue-engineering applications

Assessment of femtosecond laser induced periodic surface structures on polymer films

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