Improvement of cytotoxic and apoptogenic properties of crocin in cancer cell lines by its nanoliposomal form

Mousavi, Seyed Hadi; Moallem, Seyed Adel; Mehri, Soghra; Shahsavand, Shabnam; Nassirli, Horiyeh; Malaekeh‐Nikouei, Bizhan

doi:10.3109/13880209.2011.563315

Cited by 82 publications

(37 citation statements)

References 27 publications

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“…We and other reported that C. Sativus and its major constituent crocin is an effective in vitro anti-proliferative agent of MCF-7 cells (Mousavi et al, 2011;Bakshi et al, 2012;Bizhan Malaekeh-Nikouei et al, 2013;Samarghandian and Borji, 2014). Initiation of apoptosis and proportion of MCF-7 cells attained apoptotic state after treatment with saffron has been well enumerated by flow cytometric analysis (Mousavi et al, 2009;Mousavi et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Initiation of apoptosis and proportion of MCF-7 cells attained apoptotic state after treatment with saffron has been well enumerated by flow cytometric analysis (Mousavi et al, 2009;Mousavi et al, 2011). However, the precise role of CSE or crocin on pro-apoptotic, anti-apoptotic, and caspase signaling mediated MCF-7 cell death at molecular level is ambiguous.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro cytotoxicity of saffron on MCF-7 cells is well established by our group and others Mousavi et al, 2011;Bakshi et al, 2012;Nikouei et al, 2013;Samarghandian and Borji, 2014). Further saffron reported for initiation of apoptosis in MCF-7 cells by flow cytometric analysis (Mousavi et al, 2009;Mousavi et al, 2011). However, the precise molecular mechanism of apoptosis induced by saffron and its active constituent crocin in MCF-7 cells is unclear.…”

Section: Introductionmentioning

confidence: 89%

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Molecular Mechanism of Crocin Induced Caspase Mediated MCF-7 Cell Death: In Vivo Toxicity Profiling and Ex Vivo Macrophage Activation

Bakshi¹,

Hakkim²,

Sam³

2016

Asian Pacific Journal of Cancer Prevention

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Background: Crocus sativus and its major constituent crocin are well established to have anti-cancer properties in breast cancer cells (MCF-7). However the role of C. sativus extract (CSE) and crocin on caspase signaling mediated MCF-7 cell death at molecular level is remains unclear. In this study, we tried to unravel role of CSE and crocin on caspase mediated MCF-7 cells death and their in vivo preclinical toxicity profiling and immune stimulatory effect. Materials and Methods: CSE extract was fractionated by HPLC and crocin was isolated and characterized by NMR, IR, and MS. MCF-7 cells were treated with both CSE and crocin and expression of Bcl-2 and Bax was assessed after 24 and 36 hours. Furthermore, caspase 3, caspase 8 and caspase 9 expression was determined by Western blotting after 24 hours of treatment. DNA fragmentation analysis was performed for genotoxicity of CSE and crocin in MCF-7 cells. The in vivo toxicity profile of CSE (300 mg/kg of b.wt) was investigated in normal Swiss albino mice. In addition, peritoneal macrophages were collected from crocin (1, 1.5 and 2 mg/kg body weight) treated mice and analyzed for ex vivo yeast phagocytosis. Results: Immunoblot analysis revealed that there was time dependent decline in anti-apoptotic Bcl-2 with simultaneous upregulation of Bax in CSE and crocin treated MCF-7 cells. Further CSE and crocin treatment downregulated caspase 8 and 9 and cleaved the caspase 3 after 24 hours. Both CSE and crocin elicited considerable DNA damage in MCF-7 cells at each concentration tested. In vivo toxicity profile by histological studies revealed no observable histopathologic differences in the liver, kidney, spleen, lungs and heart in CSE treated and untreated groups. Crocin treatment elicited significant dose and time dependent ex vivo yeast phagocytosis by peritoneal macrophages. Conclusions: Our study delineated involvement of pro-apoptotic and caspase mediated MCF-7 cell death by CSE and crocin at the molecular level accompanied with extensive DNA damage. Further we found that normal swiss albino mice can tolerate the maximum dose of CSE. Crocin enhanced ex vivo macrophage yeast phagocytic ability.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 89%

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Molecular Mechanism of Crocin Induced Caspase Mediated MCF-7 Cell Death: In Vivo Toxicity Profiling and Ex Vivo Macrophage Activation

Bakshi¹,

Hakkim²,

Sam³

2016

Asian Pacific Journal of Cancer Prevention

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“…Both compounds are extremely cytotoxic in vitro towards those tumor lines. Their cytotoxicity indicates that they could be motivating as prospective antitumoral chemotherapeutics [79,80] . Indoles (I3C and DIM or its derivatives) have been revealed to induce apoptosis in breast [81][82][83][84][85][86][87] , squamous cell carcinoma [88] , cholangiocarcinoma [89] , colon [90][91][92][93] , cervical [94] , ovarian [95] , pancreatic [96,97] and prostate [98][99][100][101] cancer cells.…”

Section: Reported Indoles As Anti-cancer Active Agentsmentioning

confidence: 99%

“…The highest antipoliferative activity recorded for the carbazole derivatives in a nanomolar scale towards the three certain cancers cell lines: non-small lung cell NCIeH460 with GI = 616 nmol/L, ovarian cancer cell line OVCAR-4 with GI = 562 nmol/L and breast cancer cell line 50 nmol/L scale MCF7 with GI = 930 nmol/L (Figure 10) [16] . Figure 10 3,3′-diindolylmethane derivatives and tetrahydroindolocarbazoles Dorota et al [79,80] (lung) and SF-268(NCS) cells, considerably 5,5′-difluoro-3,3′-methanediyl-bis-indole and 5,5′-dicyano-3, 3′-methanediyl-bis-indole were tested against the MCF7 (breast), NCI-H460 (lung) and SF-268 (CNS) tumor cell lines. The results are reported as the proportion of growth of the tested cells to untested control cells (Figure 10).…”

Section: Reported Indoles As Anti-cancer Active Agentsmentioning

confidence: 99%

Indoles as anticancer agents

et al. 2015

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Saffron (Crocus sativus) in the treatment of gastrointestinal cancers: Current findings and potential mechanisms of action

Naeimi

Shafiee

Kermanshahi

et al. 2019

J of Cellular Biochemistry

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Gastrointestinal (GI) cancers are major causes of cancer‐related mortality worldwide and include malignancies of the GI tract such as the stomach, liver, pancreas, small intestine, colon, and rectum. Promising and selective anticancer effects of pharmacologically active components of saffron (Crocus sativus L.) have been shown in preclinical in vitro and in vivo studies. Saffron and its active components including crocin, crocetin, and safranal exert their anticancer effects through different mechanisms, including induction of apoptosis, influence on the cell cycle, and regulation of host immune response and anti‐inflammatory activities. This review summarizes the recent literature on the chemopreventive properties of saffron in GI cancers to have a better understanding of the potential underlying mechanisms and hence the appropriate management of these malignancies.

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Improvement of cytotoxic and apoptogenic properties of crocin in cancer cell lines by its nanoliposomal form

Abstract: It might be concluded that crocin and its liposomes could cause cell death in HeLa and MCF-7 cells, in which liposomal encapsulation improved cytotoxic effects. They could be also considered as a promising chemotherapeutic agent in cancer treatment in future.

Cited by 82 publications

References 27 publications

Molecular Mechanism of Crocin Induced Caspase Mediated MCF-7 Cell Death: In Vivo Toxicity Profiling and Ex Vivo Macrophage Activation

Molecular Mechanism of Crocin Induced Caspase Mediated MCF-7 Cell Death: In Vivo Toxicity Profiling and Ex Vivo Macrophage Activation

Indoles as anticancer agents

Saffron (Crocus sativus) in the treatment of gastrointestinal cancers: Current findings and potential mechanisms of action

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