Improvement of copper tolerance of Arabidopsis by transgenic expression of an allene oxide cyclase gene, GhAOC1, in upland cotton (Gossypium hirsutum L.)

Wang, Yuange; Liu, Huaihua; Xin, Qingguo

doi:10.1016/j.cj.2015.02.004

Cited by 11 publications

(9 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To characterize ClAOC1 and ClAOC2, a multiple sequence alignment was performed for their full-length amino acid sequences, as well as other published AOC proteins, such as SlAOC from Solanum lycopersicum [6], HvAOC from Hordeum vulgare [13], HnAOC from Hyoscyamus niger [31], JcAOC from Jatropha curcas [32], AaAOC from Artemisia annua [33], OsAOC from Oryza sativa [12], LmAOC from Leymus mollis [34], TaAOC1 from Triticum aestivum [17], GhAOC1 from Gossypium hirsutum [8], GmAOC3 from Glycine max [18], CsAOC from Camellia sinensis [35], and Ljaoc1 from Lotus japonicus [10]. The results showed that the identity between ClAOC1 and ClAOC2 was 62.13%, and the amino acid sequences of ClAOC1 and ClAOC2 shared 58.05% to 69.08% identity with other AOC proteins ( Figure 2).…”

Section: Characterization Of Claoc1 and Claoc2mentioning

confidence: 99%

“…Previous studies have revealed that the expression of AOC genes could be induced by treatments of different hormones, such as JA and SA [8,35,37]. To determine whether ClAOC1 and ClAOC2 are responsive to hormones, their expression patterns under treatments of different hormones (JA, SA, and ET) were examined by qRT-PCR.…”

Section: Expression Patterns Of Claoc1 and Claoc2 In Response To Hormmentioning

confidence: 99%

“…Several studies have shown that AOC genes compose a small gene family, and the numbers of AOC genes are different across plants. For example, there are six AOC genes in soybean [7], five in upland cotton (Gossypium hirsutum) [8], four in Arabidopsis thaliana [9], three in Lotus japonicas [10], two in Physcomitrella patens [11], while only one AOC gene in rice and tomato [6,12], respectively. In addition,…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification and Expression Analysis of Two allene oxide cyclase (AOC) Genes in Watermelon

Guang

Yang

et al. 2019

Agriculture

View full text Add to dashboard Cite

Allene oxide cyclase (AOC, EC 5.3.99.6) catalyzes the most important step in the jasmonic acid (JA) biosynthetic pathway and mediates plant defense response to a wide range of biotic and abiotic stresses. In this study, two AOC genes were identified from watermelon. Sequence analysis revealed that each of ClAOC1 and ClAOC2 contained an allene oxide cyclase domain and comprised eight highly conserved β-strands, which are the typical characteristics of AOC proteins. Phylogenetic analysis showed that ClAOC1 and ClAOC2 were clustered together with AOCs from dicotyledon, with the closest relationships with JcAOC from Jatropha curcas and Ljaoc1 from Lotus japonicus. Different intron numbers were observed in ClAOC1 and ClAOC2, which may result in their functional divergence. qRT-PCR analysis revealed that ClAOC1 and ClAOC2 have specific and complex expression patterns in multiple organs and under hormone treatments. Both ClAOC1 and ClAOC2 displayed the highest transcriptional levels in stem apex and fruit and exhibited relatively lower expression in stem. JA, salicylic acid (SA), and ethylene (ET) could enhance the expression of ClAOC1 and ClAOC2, particularly that of ClAOC2. Red light could induce the expression of ClAOC2 in root-knot nematode infected leaf and root of watermelon, indicating that ClAOC2 might play a primary role in red light-induced resistance against root-knot nematodes through JA signal pathway. These findings provide important information for further research on AOC genes in watermelon.Agriculture 2019, 9, 225 2 of 12 AOC genes were found to have specific and complicated tissue expression patterns in different plants. In barley, HvAOC mRNA accumulation is abundant in root tip, scutellar node, and leaf base [13]. Soybean GmAOC1 and GmAOC2 also exhibited abundant mRNA transcripts in roots, while GmAOC3 and GmAOC4 showed relatively higher expression in leaves and stems, respectively [7]. Camptotheca acuminata CaAOC is constitutively expressed in various organs, with the highest expression level in leaves [14]. In Arabidopsis, AtAOC1, AtAOC2, and AtAOC3 promoters exhibit high activities in the leaves, while only AtAOC3 and AtAOC4 show promoter activity in roots [15]. These results suggest that AOC genes are involved in the regulation of multiple plant developmental processes.The expression of jasmonate biosynthetic pathway genes, including AOCs, is regulated by JA, and overexpression or suppression of these genes can greatly affect the JA levels in plants. For example, a severe deficiency of jasmonate was found in two AOC mutants (cpm2 and hebiba) of rice [12], and partial suppression of MtAOC1 in hairy roots of Medicago truncatula also resulted in lower JA levels in mycorrhizal roots [16]. In addition, elevated JA levels were found in transgenic plants overexpressing AOC genes from different plants, such as AaAOC from Artemisia annua [5], TaAOC1 from wheat [17], and GmAOC3 from soybean [18]. JA and its related compounds are involved in plant defense reactions against biotic and abiotic stresses, and th...

show abstract

Section: Characterization Of Claoc1 and Claoc2mentioning

confidence: 99%

Section: Expression Patterns Of Claoc1 and Claoc2 In Response To Hormmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and Expression Analysis of Two allene oxide cyclase (AOC) Genes in Watermelon

Guang

Yang

et al. 2019

Agriculture

View full text Add to dashboard Cite

show abstract

“…In another research, GhAOC1 overexpression in Arabidopsis plants under copper stress increased their photosynthetic efficiency, survival rate, and fresh and dry weight of the stem (compared to control) and reduced the damage to the cell membrane and lipid peroxidation. The Real-time PCR analysis showed that in linseed, GhAOC transcripts are widely expressed in roots and regulated under MeJAand CuCl2 stress (Wang et al, 2015). Research's has shown that MeJA synthesis is triggered through positive feedback, as genes that encode jasmonate synthesis are JA-inducible and jasmonate increases their expression.…”

Section: Molecular Assaymentioning

confidence: 99%

“…It reported that AOC gene expression is also associated with improved copper tolerance in cotton. In Arabidopsis plants, GhAOC1 overexpression has been found to be associated with higher survival rate, higher fresh and dry weight of the shoot, improved photosynthetic efficiency and reduced cell membrane damage and lipid peroxidation under copper-induced stress (Wang et al, 2015). In two species of transgenic wheat and Arabidopsis, the TaAOC1 gene has been found to be associated with increased salinity tolerance and jasmonate accumulation (Zhao et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Effects of Methyl Jasmonate and Cadmium on Growth Traits, Cadmium Transport and Accumulation, and Allene-Oxide Cyclase Gene Expression in Wheat Seedlings

Alikhani

Abbaspour

2019

Rev. Agric. Neotrop.

View full text Add to dashboard Cite

This research investigated the toxicity effects of cadmium chloride (0, 100, 200 and 300 μM) and its interactive effects with methyl jasmonate (MeJA) (0, 0.01 and 0.1 mM) on growth parameters, cadmium (Cd) accumulation, and Allene-Oxide Cyclase (AOC) gene expression in wheat seedling (Sivand cultivar). According to the results, Cd at concentrations of 200 μM and 300 μM reduced the growth traits of the wheat. Under exposure to 300 μM Cd, root and shoot cadmium content was 30 and 17 times greater than the base level, respectively. Under 100 μM Cd exposures, bioaccumulation factor (BCF) for root and shoot increased by 46 and 25 times respectively. The spraying of MeJA on Cd-stressed plants showed a positive effect on growth parameters, increasing them by 10-40%. The treatment with 0.01 mM jasmonate decreased the accumulation of cadmium in root and shoot by about 30%. The same amount of decrease was observed in BCF after the jasmonate treatment of the specimens exposed to Cd. But application of 0.1 MeJA increased the BCF in most Cd concentrations. Under exposure to 200 μM Cd, AOC expression showed an approximately three fold increase. The use of 0.1 mM MeJA also resulted in a fourfold increase in AOC gene expression. It can conclude that MeJA showed a substantial impact on Cd accumulation and AOC gene expression in wheat seedling.Efeitos do metil jasmonato e cádmio nas características de crescimento, acúmulo e transporte de cádmio e expressão gênica da aleno óxido ciclase em mudas de trigo RESUMO Este estudo avaliou os efeitos da toxicidade do cloreto de cádmio (0, 100, 200 e 300 μM) e seus efeitos da interação com o metil jasmonato (MeJA) (0, 0,01 e 0,1 mM) sobre os parâmetros de crescimento, acúmulo de cádmio (Cd) e experessão gênica da Aleno Óxido Ciclase (AOC) em mudas de trigo (cultivar Sivand). De acordo com os resultados, o Cd nas concentrações de 200 μM e 300 μM reduziu as características de crescimento do trigo. Sob exposição a 300 μM Cd, o conteúdo de cádmio nas raízes e na parte aérea foi 30 e 17 vezes maior do que o nível básico, respectivamente. Sob exposição a 100 μM Cd, o fator de bioacumulação (FBC) para raiz e parte aérea aumentou em 46 e 25 vezes, respectivamente. A pulverização de MeJA em plantas sob estresse por Cd mostrou um efeito positivo nos parâmetros de crescimento, aumentando de 10 para 40%. O tratamento com jasmonato 0,01 mM diminuiu o acúmulo de cádmio na raiz e na parte aérea em cerca de 30%. A mesma quantidade de decréscimo foi observada no FBC após o tratamento com jasmonato nas plantas expostas ao Cd. Porém, a aplicação de 0,1 MeJA aumentou o FBC na maioria das concentrações de Cd. Sob exposição a 200 μM Cd, a expressão de AOC mostrou um aumento de aproximadamente três vezes. O uso de MeJA 0,1 mM também resultou em um aumento de quatro vezes na expressão do gene AOC. Pode-se concluir que MeJA apresentou um impacto substancial no acúmulo de Cd e na expressão do gene AOC em mudas de trigo. Palavras-chave:Triticum aestivum L., traços de crescimento, efeito de toxicidade.

show abstract

ScAOC1, an allene oxide cyclase gene, confers defense response to biotic and abiotic stresses in sugarcane

et al. 2020

View full text Add to dashboard Cite

Improvement of copper tolerance of Arabidopsis by transgenic expression of an allene oxide cyclase gene, GhAOC1, in upland cotton (Gossypium hirsutum L.)

Cited by 11 publications

References 26 publications

Identification and Expression Analysis of Two allene oxide cyclase (AOC) Genes in Watermelon

Identification and Expression Analysis of Two allene oxide cyclase (AOC) Genes in Watermelon

Effects of Methyl Jasmonate and Cadmium on Growth Traits, Cadmium Transport and Accumulation, and Allene-Oxide Cyclase Gene Expression in Wheat Seedlings

ScAOC1, an allene oxide cyclase gene, confers defense response to biotic and abiotic stresses in sugarcane

Contact Info

Product

Resources

About