Improvement of chemical analysis of antibiotics

Ikai, Yoshitomo; Oka, Hisao; Kawamura, Norihisa; Hayakawa, Junko; Yamada, Masuo; Harada, Kenichi; Suzuki, Makoto; Nakazawa, Hiroyuki

doi:10.1016/s0021-9673(01)96011-x

Cited by 43 publications

(23 citation statements)

References 13 publications

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“…Therefore, public laboratories, responsible for official control, have to provide analytical methods for determination of selected substances at the levels of interest, in both feeds and animal-derived foods. Determination of SAs was initially performed by thin-layer chromatography [12], but at present, separation and quantification of sulfonamide residues are commonly achieved by high-performance liquid chromatography [13][14][15][16][17][18][19][20][21][22][23]. For analysis of feed samples, which compared with animal tissues are a quite complex matrix, use of mass spectrometry allows multiresidue detection of SAs, ensuring accurate and sensitive determinations [24].…”

Section: Introductionmentioning

confidence: 99%

Optimization and Validation of a Confirmatory Method for Determination of Ten Sulfonamides in Feeds by LC and UV-Diode Array Detection

et al. 2011

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A simple, rapid, and reliable multiresidue method, based on liquid chromatography (LC) and ultraviolet (UV)-diode array detection (DAD), is described for assaying ten sulfonamides (sulfadiazine, sulfathiazole, sulfamerazine, sulfamethazine, sulfamethoxypyridazine, sulfamonomethoxine, sulfachloropyridazine, sulfamethoxazole, sulfaquinoxaline, and sulfadimethoxine) in feeds. The chromatographic separation is accomplished using a C18 column, eluted with a mobile phase consisting of acetate buffer, acetonitrile, and methanol. The sample preparation requires a simple extraction with chloroform/ acetone and a purification step by solid-phase extraction. The analytical parameters of precision, detection and quantification limits, recovery, and ruggedness have been evaluated by a validation procedure following the European guidelines of Regulation 882/2004/EC and Decision 657/2002/EC.

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Section: Introductionmentioning

confidence: 99%

Optimization and Validation of a Confirmatory Method for Determination of Ten Sulfonamides in Feeds by LC and UV-Diode Array Detection

et al. 2011

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“…A general approach in RP-HPLC includes sample treatment, prior to separation, by means of liquid-liquid extraction [8, 20, 27, 33, 34, 40-43, 45, 46, 48, 49], SPE [19,21,22,25,28,29,32,35,36,38,39,44,47,50], solid-phase microextraction [23,24], matrix solid-phase dispersion [26,37], supercritical fluid extraction [16][17][18]30] and polymer monolith microextraction [15,51].…”

Section: Introductionmentioning

confidence: 99%

LC of sulfonamide residues in poultry muscle and eggs extracts using fluorescence pre‐column derivatization and monolithic silica column

Zotou¹,

Vasiliadou²

2010

J of Separation Science

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A new, rapid, sensitive and selective HPLC method with fluorescence detection is described for the simultaneous determination of 12 sulfonamides, in the presence of putrescine as internal standard, after pre-column derivatization with fluorescamine. The drugs were separated on a Chromolith Performance RP-18 column (100x4.6 mm), using a gradient elution with a binary mobile phase of methanol/0.05 M acetate buffer (pH 3.4). Linearity of derivatization was obtained for concentrations from 3.0 to 300 microg/L in standard solutions. The whole procedure was evaluated and fully validated, according to the European Union Decision 2002/657/EC, for the determination of sulfonamides in turkey muscle and hen eggs following SPE. The LODs varied from 2 to 17 microg/kg in turkey and 2 to 15 microg/kg in egg samples. The average recoveries ranged between 96.9-108.6% in turkey muscle and 96.0-108.4% in egg samples, respectively.

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“…There are several methods for the determination of sulfonamides, mainly using chromatographic techniques, especially HPLC with UV, fluorescence, electrochemical detector, and MS detectors [7][8][9][10][11][12][13][14][15][16]. Recently, chemiluminescence detection has been proposed as sensitive detection for the HPLC analysis of sulfonamides of veterinary use in milk [17].…”

Section: Introductionmentioning

confidence: 99%

Application of capillary zone electrophoresis with large‐volume sample stacking to the sensitive determination of sulfonamides in meat and ground water

et al. 2006

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A CZE method with UV-Vis detection has been established and validated for the determination of nine sulfonamides: sulfapyridine, sulfamethazine, sulfamerazine, sulfamether, sulfadiazine, sulfadimethoxine, sulfamethoxazole, sulfachlorpyridazine, and sulfamethizole. Optimum separation was obtained on a 64.5 cm x 75 microm bubble cell capillary using a buffer containing 45 mM sodium phosphate and 10% methanol at pH 7.3, with temperature and voltage of 27 degrees C and 25 kV, respectively. p-Aminobenzoic acid was used as an internal standard . Taking into account the lack of sensitivity of the UV-Vis detection, the application of an on-line preconcentration methodology, such as large-volume sample stacking with polarity switching has been proposed. This procedure combined with a solvent extraction/SPE method applied for off-line preconcentration and cleanup provides a significant improvement in the LODs, ranging from 2.59 to 22.95 mug/L for the studied compounds; the quantification of these residues being possible below the levels established by EU legislation in animal food products, such as meat. Satisfactory recoveries were also obtained in the analysis of these compounds in ground water.

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Improvement of chemical analysis of antibiotics

Cited by 43 publications

References 13 publications

Optimization and Validation of a Confirmatory Method for Determination of Ten Sulfonamides in Feeds by LC and UV-Diode Array Detection

Optimization and Validation of a Confirmatory Method for Determination of Ten Sulfonamides in Feeds by LC and UV-Diode Array Detection

LC of sulfonamide residues in poultry muscle and eggs extracts using fluorescence pre‐column derivatization and monolithic silica column

Application of capillary zone electrophoresis with large‐volume sample stacking to the sensitive determination of sulfonamides in meat and ground water

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