Improvement of cellulase activity in Trichoderma reesei by heterologous expression of a beta-glucosidase gene from Penicillium decumbens

Genetic modification: a tool for enhancing cellulase secretion

“…Furthermore, heterologously expressed pBGL1 was purified and added to enzyme complex secreted by T. reesei RUT-C30 and was observed that during saccharification of pretreated cornstalk, the yield of glucose increased by up to 80%. This result showed that heterologous expression of a BGL in T. reesei could produce a balanced cellulase blend (Ma et al, 2011).…”

“…A β-glucosidase I coding sequence from P. decumbens was ligated with the CBH 1 promoter of T. reesei which is widely used in enzyme industry to enhance β-glucosidase activity in T. reesei to degrade cellulose efficiently (Ma et al, 2011). More specifically, the ligated sequences of β-glucosidase I coding sequence and CBH I promoter sequence were introduced into the genome of T. reesei RUT-C-30 through Agrobacterium-mediated transformation.…”

Increased expression of β-glucosidase A in Clostridium thermocellum 27405 significantly increases cellulase activity

“…7 Moreover, in Trichoderma reesei transformants the heterologous expression of a β-glucosidase gene from Penicillium decumbens resulted in an average 30% increase in filter paper activity (representing total cellulase activity). 19 Therefore one may hypothesize that if there is an increase in copy number of β-glucosidase in C. thermocellum, there would also be an increase in β-glucosidase activity taken when the cultures reached log and stationary phases of growth and analyzed for ethanol concentration (Fig. 4).…”

Structural characterization of an endoglucanase from Serpula lacrymans var. lacrymans S7.9

“…It is generally recognized that one of the problems in cellulose hydrolysis is the slowdown of enzyme activity in time and at high conversions [8]. Nowadays the efforts to improve the activity and productivity of cellulases are at the research forefront for bioenergy technology [4]. Many details of the conversion processes including pretreatment and enzymatic hydrolysis are not completely understood.…”

“…Biomass energy is a promising option of renewable energy but the feedstock used for producing biomass energy should come from non-food biomass or agricultural waste [3]. Lignocellulosic biomass is the most abundant sustainable natural resource on the earth available for alternative biofuels [4]. Cellulosic ethanol as a substitute for fossil fuels has attracted the widespread interests [5].…”