Improvement of Cardiac Puncture in Mice

Ohwada, Kazuo

doi:10.1538/expanim1978.35.3_353

Cited by 20 publications

(10 citation statements)

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“…22 Serum vIL-10 assay Rats were placed under general anesthesia, and blood samples were obtained from the heart according to the method of Ohwada. 46 Serum vIL-10 was measured by ELISA as follows. Plates (96-wells) were coated with 2 mg/ml of rat anti-human and anti-vIL-10 monoclonal antibody (PharMingen, Carlsbad, CA, USA) at 41C overnight, washed with PBS containing 0.05% Tween 20 (PBS/Tween), and blocked by incubation with PBS containing 1% bovine serum albumin at room temperature for 1 h. After washing with PBS/Tween, appropriately diluted samples were added to the wells.…”

Section: Plasmid Dna Injectionmentioning

confidence: 99%

Hydrodynamics-based delivery of the viral interleukin-10 gene suppresses experimental crescentic glomerulonephritis in Wistar–Kyoto rats

et al. 2003

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Gene therapy is expected to revolutionize the treatment of kidney diseases. Viral interleukin (vIL)-10 has a variety of immunomodulatory properties. We examined the applicability of vIL-10 gene transfer to the treatment of rats with crescentic glomerulonephritis, a T helper 1 (Th 1) predominant disease. To produce the disease, Wistar-Kyoto rats were injected with a rabbit polyclonal anti-rat glomerular basement membrane antibody. After 3 h, a large volume of plasmid DNA expressing vIL-10 (pCAGGS-vIL-10) solution was rapidly injected into the tail vein. pCAGGS solution was similarly injected into control rats (pCAGGS rats). We confirmed the presence of vector-derived vIL-10 mainly in the liver and observed high serum vIL-10 levels in pCAGGSvIL-10-injected rats. Compared with the pCAGGS rats, the pCAGGS-vIL-10 rats showed significant therapeutic effects: reduced frequency of crescent formation, decrease in the number of total cells, macrophages, and CD4 + T cells in the glomeruli, decrease in urine protein, and attenuation of kidney dysfunction. Using quantitative real-time polymerase chain reaction, we also observed that this model was Th1-predominant in the glomeruli and that the ratio of the transcripts of CD4, interferon-g, tumor necrosis factor-a, and monocyte chemotactic protein-1 to the transcripts of glucose-6-phosphate dehydrogenase in the glomeruli were all significantly lower in the pCAGGS-vIL-10 rats than in the pCAGGS rats. These results demonstrate that pCAGGS-vIL-10 gene transfer by hydrodynamics-based transfection suppresses crescentic glomerulonephritis.

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Section: Plasmid Dna Injectionmentioning

confidence: 99%

Hydrodynamics-based delivery of the viral interleukin-10 gene suppresses experimental crescentic glomerulonephritis in Wistar–Kyoto rats

et al. 2003

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“…30 Serum rat Epo levels were determined using a Recombigen EPO kit (Nippon, Chiba, Japan), which is a radioimmunoassay with a rabbit polyclonal antibody against Epoetin ␣. This kit has a linear range of measurement between 3.0 and 200 mU/ml of human Epo with a detection threshold of 3.0 mU/ml.…”

Section: Blood Parametersmentioning

confidence: 99%

Long-term production of erythropoietin after electroporation-mediated transfer of plasmid DNA into the muscles of normal and uremic rats

et al. 2001

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The anemia associated with chronic renal failure is one of the best target diseases for erythropoietin (Epo) gene transfer. We previously reported a short-term (1 month) study of continuous rat Epo delivery by muscle-targeted gene transfer of plasmid DNA expressing rat Epo (pCAGGS-Epo) using in vivo electroporation in normal rats. Here, we performed a long-term pharmacokinetic study of continuous Epo delivery by this method in normal rats and uremic five-sixths nephrectomized rats. In normal rats, Epo gene expression and sufficient erythropoiesis occurred with Epo gene transfer in a dose-dependent manner, and persisted for at least 11

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“…Res., 10 (2): 37-43, 2015 Collection of blood samples and measurement of biochemical parameters: The animals were made unconscious using chloroform anesthesia. The blood samples were collected via cardiac puncture, a method modified by Ohwada (1986). A 5 mL syringe, attached to a sterilized needle was used to collect the blood samples from the heart.…”

Section: Preparation Of the Aqueous Extractmentioning

confidence: 99%

Alteration of Lipid Profile and Serum Electrolytes Following Chronic Consumption of Periwinkle Extract in Rats

Ak¹,

Archibong²,

Ofem³

et al. 2015

Trends in Medical Research

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Periwinkles are important edible seafood essential to life. They are cheap and easily affordable sources of protein, although they are harvested from river waters that are made of variety of pollutants. This research therefore sought to investigate any possible alterations in some biochemical parameters of albino Wistar rats by periwinkle intake. Fifteen albino Wistar rats weighing between 200-250 g were assigned into 3 groups of 5 rats each and housed singly in metabolic cages. The rats took normal rat pellets and drinking water ad libitum. The 2 test groups received different doses (low dose -7.0 mg mLG 1 and high dose -52 mg mLG 1 ) of periwinkle extract orally, once daily. The control group received a daily oral administration of 0.6 mL of normal saline as vehicle. The feeding regimens lasted for 6 weeks, after which blood samples were collected for estimation of some biochemical parameters. Results showed that mean values of the lipid profile in control group were: TC (1.44±0.05 mg dLG when compared with control group. The periwinkle extract fed rats also had significant reduction in Na + (p<0.001) and ClG (p<0.001) concentrations compared with the control. Total cholesterol, triglyceride, LDL-c and VLDL-c concentrations were also significantly (p<0.001) reduced in the periwinkle extract fed groups compared with control. Previous study had shown that periwinkle (T. fuscatus) contains omega 3 fatty acid, which could be attributed to the beneficial effect of the extract on lipid profile and serum electrolytes observed in this study. Hence, chronic consumption of periwinkle could help alleviate dyslipidemia and other conditions associated with deleterious alterations in electrolyte concentrations. This beneficial effect of the extract on lipid profile and serum electrolytes was supported by multivariate analysis, which showed that statistically there was a significant difference in lipid profile among the different groups, F (10, 16) = 15.578, p<0.0001; Will's Lambda = 0.009, partial Eta Sq. = 0.907 and in serum electrolyte concentrations among the different groups, F (10, 16) = 222.958, p<0.0001; Wilk's Lambda = 0.000, partial Eta Sq. = 0.993.

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Improvement of Cardiac Puncture in Mice

Cited by 20 publications

References 0 publications

Hydrodynamics-based delivery of the viral interleukin-10 gene suppresses experimental crescentic glomerulonephritis in Wistar–Kyoto rats

Hydrodynamics-based delivery of the viral interleukin-10 gene suppresses experimental crescentic glomerulonephritis in Wistar–Kyoto rats

Long-term production of erythropoietin after electroporation-mediated transfer of plasmid DNA into the muscles of normal and uremic rats

Alteration of Lipid Profile and Serum Electrolytes Following Chronic Consumption of Periwinkle Extract in Rats

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