Improvement of calcium phosphate scaffold osteogenesis in vitro via combination of glutamate-modified BMP-2 peptides

Cao, Qinggang; He, Zhiwei; Sun, Wendell Q.; Fan, Gentao; Zhao, Jianning; Bao, Nirong; Ye, Tingjun

doi:10.1016/j.msec.2018.11.048

Cited by 13 publications

(9 citation statements)

References 25 publications

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“…ALP and Runx2 are often used as markers of osteogenic differentiation and are expressed by BMSCs in the early stage. 57,58 OPN plays important roles in osteogenic differentiation in later stages. 59,60 The expression of ALP, OPN, and Runx 2 in the SK group was higher than that in the control group at day 7 and 14 in this study.…”

Section: Discussionmentioning

confidence: 99%

Controlled Delivery of Growth Factor by Hierarchical Nanostructured Core–Shell Nanofibers for the Efficient Repair of Critical-Sized Rat Calvarial Defect

Huang

Yang

Zhou

et al. 2020

ACS Biomater. Sci. Eng.

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Electrospun nanofibers have received much attention as bone tissue-engineered scaffolds for their capacity to mimic the structure of natural extracellular matrix (ECM). Most studies have reproduced nanofibers with smooth surface for tissue engineering. This is quite different from the triple-helical nanotopography of natural collagen nanofibrils. In this study, hierarchical nanostructures were coated on the surface of drug-loaded core–shell nanofibers to mimic natural collagen nanofibrils. The nanoshish-kebab (SK) structure was decorated regularly on the surface of the nanofibers, and the inner-loaded bone morphogenetic protein 2 (BMP2) exhibited a gentle release pattern, similar to a zero-order release pattern in kinetics. The in vitro study also showed that the SK structure could accelerate cell proliferation, attachment, and osteogenic differentiation. Four groups of scaffolds were implanted in vivo to repair critical-sized rat calvarial defects: (1) PCL/PVA (control); (2) SK-PCL/PVA; (3) PCL/PVA-BMP2; and (4) SK-PCL/PVA-BMP2. Much more bone was formed in the SK-PCL/PVA group (24.57 ± 3.81%) than in the control group (1.21 ± 0.23%). The BMP2-loaded core–shell nanofibers with nanopatterned structure (SK-PCL/PVA-BMP2) displayed the best repair efficacy (76.38 ± 4.13%), followed by the PCL/PVA-BMP2 group (39.86 ± 5.74%). It was believed that the hierarchical nanostructured core–shell nanofibers could promote osteogeneration and that the SK structure showed synergistic ability with nanofiber-loaded BMP2 in vivo for bone regeneration. Thus, this BMP2-loaded core–shell nanofiber scaffold with hierarchical nanostructure holds great potential for bone tissue engineering applications.

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Section: Discussionmentioning

confidence: 99%

Controlled Delivery of Growth Factor by Hierarchical Nanostructured Core–Shell Nanofibers for the Efficient Repair of Critical-Sized Rat Calvarial Defect

Huang

Yang

Zhou

et al. 2020

ACS Biomater. Sci. Eng.

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“…It has been established that the porous structure of calcium phosphate material can significantly influence bone regeneration (Xie et al, 2016;Bastami et al, 2017;Seong et al, 2020;Zamani et al, 2021). The porous surface of bone materials enhances the mechanical interlocking between the bone substitute and the surrounding bone tissues, providing mechanical stability to the bone-material interface (Meka et al, 2019;Roopavath et al, 2019;Cao et al, 2019;Dos Santos Trento et al, 2020). Two different range of pore sizes have generally been studied: micropore (<5 μm in diameter) and macropore (>100 μm in diameter) (Blokhuis et al, 2000).…”

Section: The Influence Of Physical Properties Of Beta-tricalcium Phosphate On Osteogenesismentioning

confidence: 99%

Current Application of Beta-Tricalcium Phosphate in Bone Repair and Its Mechanism to Regulate Osteogenesis

Zhou

et al. 2021

Front. Mater.

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Large segmental bone loss and bone resection due to trauma and/or the presence of tumors and cysts often results in a delay in healing or non-union. Currently, the bone autograft is the most frequently used strategy to manage large bone loss. Nevertheless, autograft harvesting has limitations, namely sourcing of autograft material, the requirement of an invasive procedure, and susceptibility to infection. These disadvantages can result in complications and the development of a bone substitute materials offers a potential alternative to overcome these shortcomings. Among the biomaterials under consideration to date, beta-tricalcium phosphate (β-TCP) has emerged as a promising material for bone regeneration applications due to its osteoconductivity and osteoinductivity properties as well as its superior degradation in vivo. However, current evidence suggests the use β-TCP can in fact delay bone healing and mechanisms for this observation are yet to be comprehensively investigated. In this review, we introduce the broad application of β-TCP in tissue engineering and discuss the different approaches that β-TCP scaffolds are customized, including physical modification (e.g., pore size, porosity and roughness) and the incorporation of metal ions, other materials (e.g., bioactive glass) and stem cells (e.g., mesenchymal stem cells). 3D and 4D printed β-TCP-based scaffolds have also been reviewed. We subsequently discuss how β-TCP can regulate osteogenic processes to aid bone repair/healing, namely osteogenic differentiation of mesenchymal stem cells, formation of blood vessels, release of angiogenic growth factors, and blood clot formation. By way of this review, a deeper understanding of the basic mechanisms of β-TCP for bone repair will be achieved which will aid in the optimization of strategies to promote bone repair and regeneration.

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“…[44], PH20 hyaluronidase [56], SIRPα [57], cytochrome C [97], enhanced green fluorescent protein (eGFP) [116], GOx [119], HA-modified RNase A [136], E7-bone morphogenetic protein 2 (BMP2) [137] and rhBMP2 [138].…”

Section: Iii113 Bicinchoninic Acid Assaymentioning

confidence: 99%

“…For inorganic-based MacPSs, Cao et al proposed to use commercial α-TCP scaffold for the loading and release of a BMP2 peptide via E7 domain [137]. The E7 domain, a natural part of bone sialoprotein, was covalently linked to the BMP2 peptide KIPKASSVPTELSAISTLYL and allowed the loading via its binding to Ca 2+ on the scaffold.…”

Section: V111 Single Formulation: Organic and Inorganic Material Alone Or In Combinationmentioning

confidence: 99%

Design and applications of protein delivery systems in nanomedicine and tissue engineering

Bizeau

Mertz

2021

Advances in Colloid and Interface Science

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Improvement of calcium phosphate scaffold osteogenesis in vitro via combination of glutamate-modified BMP-2 peptides

Cited by 13 publications

References 25 publications

Controlled Delivery of Growth Factor by Hierarchical Nanostructured Core–Shell Nanofibers for the Efficient Repair of Critical-Sized Rat Calvarial Defect

Controlled Delivery of Growth Factor by Hierarchical Nanostructured Core–Shell Nanofibers for the Efficient Repair of Critical-Sized Rat Calvarial Defect

Current Application of Beta-Tricalcium Phosphate in Bone Repair and Its Mechanism to Regulate Osteogenesis

Design and applications of protein delivery systems in nanomedicine and tissue engineering

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