Improvement of blast resistance of the popular high-yielding, medium slender-grain type, bacterial blight resistant rice variety, Improved Samba Mahsuri by marker-assisted breeding

Rekha, G.; Kumar, V. Abhilash; Viraktamath, B. C.; Pranathi, K.; Kousik, M. B. V. N.; Prasanna, B. Laxmi; Backiyalakshmi, C.; Sinha, Pragya; Ravindra, Rudravajhala; Bhaskar, S.; Hajira, S. K.; Balachiranjeevi, C. H.; Swapnil, K.; Rambabu, R.; Harika, G.; Punniakotti, E.; Anila, M.; Mahadev, H. K.; Kumar, T. Dilip; Yugander, A.; Chaitra, K.; Praveen, M; Madhavi, K. R.; Prasad, M. S.; Laha, G. S.; Neeraja, C. N.; Balachandran, S. M.; Senguttuvel, P.; Fiyaz, R. Abdul; Badri, Jyothi; Giri, Archana; Rao, L. V. Subba; Babu, V. Ravindra; Sundaram, R. M.

doi:10.1007/s13562-018-0455-9

Cited by 16 publications

(9 citation statements)

References 30 publications

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“…Phenotypic screening using a virulent isolate of bacterial blight pathogen confirmed the earlier findings that the recurrent parent, ISM possesses high levels of resistance against bacterial blight disease (Rekha et al 2018a). In fact, during the development of DDR Dhan 58, we selected lines with the genetic background of ISM, showing a high degree of bacterial blight resistance with a lesion length of 0.3 to 1.7 cm against Xoo with a score of 0-3, while FL478 showed susceptible reaction to bacterial blight disease, similar to previous reports (Rajpurohit et al 2011;Dokku et al 2013;Suh et al 2013;Pradhan et al 2015).…”

Section: Discussionsupporting

confidence: 73%

“…Genomic DNA extraction from the leaf tissues, collected from the parental lines and the backcross derived lines was carried out using modified CTAB method as explained in Sundaram et al ( 2009 ). PCR amplification, gel electrophoresis and visualization of markers specific amplicons for target genes/QTL as per Rekha et al ( 2018a ) with respect to the bacterial blight resistance genes and as per Sundaram et al ( 2009 ) for the SSR markers specific for Saltol QTL. The genome-wide polymorphism survey was carried out with a total of 200 SSR markers selected from Gramene ( https://archive.gramene.org/markers/microsat/all-ssr.tab ) and uniformly distributed across all 12 rice chromosomes.…”

Section: Methodsmentioning

confidence: 99%

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DRR Dhan 58, a Seedling Stage Salinity Tolerant NIL of Improved Samba Mahsuri Shows Superior Performance in Multi-location Trials

Rekha

Kumar

Gokulan

et al. 2022

Rice

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Background Improved Samba Mahsuri (ISM) is an elite, high-yielding, bacterial blight resistant, fine-grained rice variety with low glycaemic index. It is highly sensitive to salt stress, particularly at seedling stage, which significantly reduces its yield potential in coastal areas. A salinity tolerant QTL, Saltol, associated with seedling stage tolerance was previously mapped on chromosome 1 (10.6–11.5 Mb) from the Indian landrace, Pokkali and is effective in different genetic backgrounds. The objective of this study was to enhance salinity tolerance of ISM by incorporating the Saltol QTL through marker-assisted backcross breeding using the breeding line, FL478 (Pokkali/IR29). Results Foreground selection was carried out at each generation using five Saltol-specific markers and three bacterial blight resistance genes, Xa21, xa13 and xa5. Background selection was conducted using 66 well distributed polymorphic SSR markers and at the BC3F2 generation, a single plant with maximum recurrent parent genome recovery (95.3%) was identified and advanced to the BC3F4 generation. Based on bacterial blight resistance, seedling stage salinity tolerance and resemblance to ISM, four advanced breeding lines were selected for testing in replicated experiments near Hyderabad, India. A promising near-isogenic line, DRR Dhan 58, was evaluated in multi-location trials-coastal salinity and it showed significant salinity tolerance, resistance to bacterial blight disease, high yield and excellent grain quality during the 2019 and 2020 trials. DRR Dhan 58 was 95.1% similar to ISM based on genotyping with the 90 K SNP chip. Whole genome resequencing analysis of Pokkali and FL478 which were salinity tolerant checks, ISM and DRR Dhan 58 showed a high degree of relatedness with respect to the candidate gene loci for Saltol and OsSKC1 (Shoot K+Concentration 1). Conclusion DRR Dhan 58, possessing Saltol and three bacterial blight resistance genes (Xa21, xa13 and xa5) in the genetic background of the Indian mega-variety of rice, Samba Mahsuri, was developed for potential cultivation in areas prone to seedling stage salinity, as well as areas with endemic bacterial blight disease. This entry had a 24% yield advantage over the recurrent parent ISM under coastal saline conditions in multi-location trials and was recently released for commercial cultivation in India.

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Section: Discussionsupporting

confidence: 73%

Section: Methodsmentioning

confidence: 99%

DRR Dhan 58, a Seedling Stage Salinity Tolerant NIL of Improved Samba Mahsuri Shows Superior Performance in Multi-location Trials

Rekha

Kumar

Gokulan

et al. 2022

Rice

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“…These markers were also used by Ramalingam et al (2017) and Rekha et al (2018). The strategy of combining phenotyping with genotyping for BB resistance in the later generation of backcrossing/sel ng has been reported earlier by Hari et al (2011;, wherein a stringent phenotypic screening initially followed by selective genotyping of resistant plants has been reported to save time and resources.…”

Section: Resultsmentioning

confidence: 99%

Improvement of bacterial blight resistance of the popular variety, Nellore Mahsuri (NLR34449) through marker-assisted breeding

Dasari

Vemulapalli

Gonuguntla

et al. 2022

J Genet

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To combat the dreaded diseases in rice like bacterial blight and blast, host plant resistance has been advocated as a sustainable method. Through the present study, we have successfully incorporated three major bacterial blight (BB) resistance genes viz., Xa21, xa13 and xa5 into NLR3449, a high yielding, blast resistant, ne-grain type popular rice variety through marker-assisted backcross breeding. Foreground selection was carried out using PCR based, gene-speci c markers viz., pTA248 (Xa21), xa13prom (xa13) and xa5FM (xa5) at each generation of backcrossing, while 127 polymorphic SSR markers spanning on 12 chromosomes were used for background selection and backcrossing was limited to two rounds. At BC 2 F 1 generation, a single plant (NLR-87-10) with 89.9% recovery and possessing all the three bacterial blight resistance genes was forwarded to BC 2 F 2 generation. A solitary BC 2 F 2 plant viz., NLR-87-10-106 possessing all the three resistance genes and > 90% genome recovery was identi ed and advanced through sel ng till BC 2 F 4 generation by adopting pedigree method. Three best lines at BC 2 F 4 lines, possessing high level of resistance against bacterial blight and blast and equivalent or superior to NLR 34449 in terms of yield, grain quality and agro-morphological traits have been identi ed and advanced for multi-location trials.

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“…Gene pyramiding with two or more genes is successful as per the studies Sundaram et al [9] and Rekha et al [14], but a single major BB resistance gene like Xa21 have shown the desired level of resistance against the known isolates of BB pathogen. Similar results of single gene conferring resistance was observed with Hari et al [10], Balachiranjeevi et al [11], Abhilash et al [12] and Laxmi Prasanna et al [13] and the known isolates of BB pathogen confers good level of resistance.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic Evaluation of Advanced Breeding Lines for Resistance against Bacterial Leaf Blight Disease in Rice (Oryza sativa L.)

Srujana

Marathi²,

Srinivas³

et al. 2021

IJECC

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Bacterial Leaf Blight disease epidemically damaged the rice crop. Absence of resistance against BLB disease in MTU1010 is one of the main reason for these epidemics. Thus, there is urgent need to search for resistant source and subsequently, their phenotypic evaluation to validate the resistant source. In this paper discussing about the highlights of phenotypic evaluation of Bacterial Leaf Blight resistance in MTU1010 NIL variety of rice. In the present study, fifty advanced breeding lines along with parents i.e., MTU1010 NIL X Akshayadhan NIL, were screened for bacterial leaf blight disease, Thirty-four breeding lines were shown resistance against bacterial leaf blight disease.

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Improvement of blast resistance of the popular high-yielding, medium slender-grain type, bacterial blight resistant rice variety, Improved Samba Mahsuri by marker-assisted breeding

Cited by 16 publications

References 30 publications

DRR Dhan 58, a Seedling Stage Salinity Tolerant NIL of Improved Samba Mahsuri Shows Superior Performance in Multi-location Trials

DRR Dhan 58, a Seedling Stage Salinity Tolerant NIL of Improved Samba Mahsuri Shows Superior Performance in Multi-location Trials

Improvement of bacterial blight resistance of the popular variety, Nellore Mahsuri (NLR34449) through marker-assisted breeding

Phenotypic Evaluation of Advanced Breeding Lines for Resistance against Bacterial Leaf Blight Disease in Rice (Oryza sativa L.)

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