Improvement of biological age by physical activity

Melk, Anette; Tegtbur, Uwe; Hilfiker‐Kleiner, Denise; Eberhard, Jörg; Saretzki, Gabriele; Eulert, Christine; Kerling, Arno; Nelius, Anne-Katrin; Hömme, Meike; Strunk, Daniel R.; Berliner, Dominik; Röntgen, Philipp; Kück, Momme; Bauersachs, Johann; Hilfiker, Andres; Haverich, Axel; Bara, Christoph; Stiesch, Meike

doi:10.1016/j.ijcard.2014.07.236

Cited by 33 publications

(24 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Quantification of the telomere length is a well-established method to detect cell senescence [13, 14]. It could be shown previously that a longer donor leukocyte telomere length is associated with improved survival after haematopoietic cell transplantation, e.g., in severe aplastic anaemia [17, 18].…”

Section: Discussionmentioning

confidence: 99%

“…DNA quantification was performed on an Infinite M200 Pro microplate reader (Tecan, Männedorf, Switzerland) using SYBR®Green I Dye (Invitrogen, Carlsbad, CA, USA). Telomere length was calculated as the abundance of telomeric template versus a single copy gene (36B4) by quantitative real-time PCR on a 7900HT fast real-time PCR system (Applied Biosystems, Darmstadt, Germany) as previously described [13, 14]. Results are presented as the ratio (T/S) between the telomere repeat copy number (T) to the single gene copy number (S).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Functionality and Cell Senescence of CD4/ CD8-Selected CD20 CAR T Cells Manufactured Using the Automated CliniMACS Prodigy® Platform

Aleksandrova

Leise

Priesner

et al. 2019

Transfus Med Hemother

Self Cite

View full text Add to dashboard Cite

Clinical studies using autologous CAR T cells have achieved spectacular remissions in refractory CD19+ B cell leukaemia, however some of the patient treatments with CAR T cells failed. Beside the heterogeneity of leukaemia, the distribution and senescence of the autologous cells from heavily pretreated patients might be further reasons for this. We performed six consecutive large-scale manufacturing processes for CD20 CAR T cells from healthy donor leukapheresis using the automated CliniMACS Prodigy® platform. Starting with a CD4/CD8-positive selection, a high purity of a median of 97% T cells with a median 65-fold cell expansion was achieved. Interestingly, the transduction rate was significantly higher for CD4+ compared to CD8+ T cells and reached in a median of 23%. CD20 CAR T cells showed a good specific IFN-γ secretion after cocultivation with CD20+ target cells which correlated with good cytotoxic activity. Most importantly, 3 out of 5 CAR T cell products showed an increase in telomere length during the manufacturing process, while telomere length remained consistent in one and decreased in another process. In conclusion, this shows for the first time that beside heterogeneity among healthy donors, CAR T cell products also differ regarding cell senescence, even for cells manufactured in a standardised automated process.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Functionality and Cell Senescence of CD4/ CD8-Selected CD20 CAR T Cells Manufactured Using the Automated CliniMACS Prodigy® Platform

Aleksandrova

Leise

Priesner

et al. 2019

Transfus Med Hemother

Self Cite

View full text Add to dashboard Cite

show abstract

“…In contrast, both DR and rapamycin feeding increased mitochondrial TERT but had no effects on TERT abundance in whole brain homogenates. It had been shown previously that various stimuli that mimic aspects of DR, such as resveratrol and exercise, can increase cellular telomerase activity and TERT expression in different human cell types such as endothelial progenitor cells in vitro and PBMCs in vivo [54, 55] and might thus have beneficial anti-aging effects. However, the DR mimetic rapamycin has been reported to decrease telomerase activity and TERT protein levels in cancer cells in the absence of transcriptional changes in TERT expression [56].…”

Section: Discussionmentioning

confidence: 99%

Decreased mTOR signalling reduces mitochondrial ROS in brain via accumulation of the telomerase protein TERT within mitochondria

Miwa

Czapiewski

Wan

et al. 2016

Aging

Self Cite

View full text Add to dashboard Cite

“…Exercise training (and physical activity) is capable of reversing some of the effects of ageing, such as loss of muscle mass or function, by promoting the expression of myofibrillar proteins. Regular exercise is associated with maintaining longer telomeres [267]. Moreover, exercise improves glucose metabolism and increases insulin sensitivity [268].…”

Section: Potential Treatment For Ageingmentioning

confidence: 99%

Ageing and the border between health and disease

2014

View full text Add to dashboard Cite

Ageing is associated with a progressive degeneration of the tissues, which has a negative impact on the structure and function of vital organs and is among the most important known risk factors for most chronic diseases. Since the proportion of the world's population aged .60 years will double in the next four decades, this will be accompanied by an increased incidence of chronic age-related diseases that will place a huge burden on healthcare resources.There is increasing evidence that many chronic inflammatory diseases represent an acceleration of the ageing process. Chronic pulmonary diseases represents an important component of the increasingly prevalent multiple chronic debilitating diseases, which are a major cause of morbidity and mortality, particularly in the elderly. The lungs age and it has been suggested that chronic obstructive pulmonary disease (COPD) is a condition of accelerated lung ageing and that ageing may provide a mechanistic link between COPD and many of its extrapulmonary effects and comorbidities. In this article we will describe the physiological changes and mechanisms of ageing, with particular focus on the pulmonary effects of ageing and how these may be relevant to the development of COPD and its major extrapulmonary manifestations. @ERSpublications Premature ageing of the lung may constitute pathogenetic mechanisms contributing to COPD

show abstract

Improvement of biological age by physical activity

Cited by 33 publications

References 9 publications

Functionality and Cell Senescence of CD4/ CD8-Selected CD20 CAR T Cells Manufactured Using the Automated CliniMACS Prodigy® Platform

Functionality and Cell Senescence of CD4/ CD8-Selected CD20 CAR T Cells Manufactured Using the Automated CliniMACS Prodigy® Platform

Decreased mTOR signalling reduces mitochondrial ROS in brain via accumulation of the telomerase protein TERT within mitochondria

Ageing and the border between health and disease

Contact Info

Product

Resources

About