Improvement of beauvericin production by Fusarium oxysporum AB2 under solid-state fermentation using an optimised liquid medium and co-cultures

Vásquez-Bonilla, J Norberto; Barranco-Florido, Juan Esteban; Ponce‐Alquicira, Edith; Rincón-Guevara, Mónica A.; Loera, Octavio

doi:10.1007/s12550-022-00458-y

Cited by 4 publications

(3 citation statements)

References 44 publications

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“…Other than the ecological role of Fusarium in soil, co-cultures with other species have also revealed their great potential as an inducer strain, in which the chemical and biological outcomes are highly dependent on the challenged species. Co-cultivation with Alternaria tenuissima , Sarocladium strictum , Saccharopolyspora erythraea, Streptomyces lividans, Epicoccum nigrum , or Bacillus subtilis has led to increased production of trichothecenes ( Müller et al, 2012 ), polyketides ( Bohni et al, 2016 ), decalin-type tetramic acid analogs ( Whitt et al, 2014 ), ennantins ( Ola et al, 2013 ; Moussa et al, 2019 ; Vásquez-Bonilla et al, 2022 ), lateropyrone, naphthoquinones, lipopeptides ( Moussa et al, 2019 ) and coumarins ( Ola et al, 2013 ), illustrating the chemodiversity of the Fusarium species. Furthermore, Fusarium species can also act as a challenge strain, causing induction of several BCGs in Ustilago maydis ( Estrada et al, 2011 ), A. giganteus ( Meyer and Stahl, 2003 ), Botrytis cinereal ( Serrano et al, 2017 ), A. tenuissima ( Müller et al, 2012 ), or P aeruginosa ( Moussa et al, 2020 ).…”

Section: Microorganisms In Mixed Culturesmentioning

confidence: 99%

Enhancing chemical and biological diversity by co-cultivation

Selegato

Castro‐Gamboa

2023

Front. Microbiol.

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In natural product research, microbial metabolites have tremendous potential to provide new therapeutic agents since extremely diverse chemical structures can be found in the nearly infinite microbial population. Conventionally, these specialized metabolites are screened by single-strain cultures. However, owing to the lack of biotic and abiotic interactions in monocultures, the growth conditions are significantly different from those encountered in a natural environment and result in less diversity and the frequent re-isolation of known compounds. In the last decade, several methods have been developed to eventually understand the physiological conditions under which cryptic microbial genes are activated in an attempt to stimulate their biosynthesis and elicit the production of hitherto unexpressed chemical diversity. Among those, co-cultivation is one of the most efficient ways to induce silenced pathways, mimicking the competitive microbial environment for the production and holistic regulation of metabolites, and has become a golden methodology for metabolome expansion. It does not require previous knowledge of the signaling mechanism and genome nor any special equipment for cultivation and data interpretation. Several reviews have shown the potential of co-cultivation to produce new biologically active leads. However, only a few studies have detailed experimental, analytical, and microbiological strategies for efficiently inducing bioactive molecules by co-culture. Therefore, we reviewed studies applying co-culture to induce secondary metabolite pathways to provide insights into experimental variables compatible with high-throughput analytical procedures. Mixed-fermentation publications from 1978 to 2022 were assessed regarding types of co-culture set-ups, metabolic induction, and interaction effects.

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Section: Microorganisms In Mixed Culturesmentioning

confidence: 99%

Enhancing chemical and biological diversity by co-cultivation

Selegato

Castro‐Gamboa

2023

Front. Microbiol.

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“…Beauvericin belongs to the cyclic hexadepsipeptide family and is produced via a non-ribosomal pathway utilizing beauvericin synthetase. It sequentially binds hydroxy isovaleric acid and N-methyl-phenylalanine molecules [69]. Certain entomopathogenic fungi generate Beauvericin, which exhibits diverse biological activities, including insecticidal, antimicrobial, and antitumor properties.…”

Section: Methylerythritol-phosphate (Mep) Pathwaymentioning

confidence: 99%

“…Due to the intricate nature of its chemical synthesis, beauvericin production is predominantly accomplished via in vivo biosynthesis using specialized producer strains. Recently, Vásquez-Bonilla et al [69] reported an enhancement in beauvericin production using solid-state cultures of Fusarium oxysporum AB2 compared to liquid cultures, increasing the yield from 0.8 mg/L to 65.3 mg/L. Moreover, they further improved yields by employing mixed cultures of F. oxysporum AB2 and Epicoccum nigrum TORT, producing 84.6 mg/L.…”

Section: Methylerythritol-phosphate (Mep) Pathwaymentioning

confidence: 99%

Strategies for Supplying Precursors to Enhance the Production of Secondary Metabolites in Solid-State Fermentation

Méndez-Hernández,

Rodríguez-Durán,

Páez-Lerma

et al. 2023

Fermentation

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The production of secondary metabolites can be improved with the supply of precursors both in submerged and solid-state fermentation (SSF). Microorganisms assimilate the precursors and biotransform them to excrete compounds of commercial interest. The raw materials used in SSF, frequently agro-industrial residues, may contain molecules that serve as precursors for secondary metabolites. However, supplying a precursor can dramatically improve crop production. Commonly, precursors are added as part of the liquid with which the solid material to be fermented is moistened. However, recently it has been proposed to take advantage of the oxygen supply for the gradual supply of volatile precursors. It can help to avoid toxicity problems with the precursors. The present work reviews the strategies to supply precursors to improve the production of secondary metabolites in solid-state fermentation.

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