Improvement of bacterial cellulose production by manipulating the metabolic pathways in which ethanol and sodium citrate involved

Li, Yuanjing; Tian, Chunjie; Tian, Hua; Zhang, Jiliang; He, Xin; Ping, Wenxiang; Lei, Hong

doi:10.1007/s00253-012-4242-6

Cited by 81 publications

(56 citation statements)

References 37 publications

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“…Bacterial cellulose (BC) is an extracellular polysaccharide secreted mainly by Gluconacetobacter xylinus, a rod-shaped, strictly aerobic gram-negative bacterium (Moosavi-Nasab and Yousefi 2011; Li et al 2012). This microorganism produces a pellicle of BC that has a nanofibrillar structure, with a denser surface on one side and a gelatinous layer on the other side (Klemm et al 2001;Kurosumi et al 2009;Cai and Kim 2010;Nakagaito et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Bacterial cellulose production by Gluconacetobacter xylinus by employing alternative culture media

Jozala

Pértile

Santos

et al. 2014

Appl Microbiol Biotechnol

159

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Bacterial cellulose (BC) is used in different fields as a biological material due to its unique properties. Despite there being many BC applications, there still remain many problems associated with bioprocess technology, such as increasing productivity and decreasing production cost. New technologies that use waste from the food industry as raw materials for culture media promote economic advantages because they reduce environmental pollution and stimulate new research for science sustainability. For this reason, BC production requires optimized conditions to increase its application. The main objective of this study was to evaluate BC production by Gluconacetobacter xylinus using industry waste, namely, rotten fruits and milk whey, as culture media. Furthermore, the structure of BC produced at different conditions was also determined. The culture media employed in this study were composed of rotten fruit collected from the disposal of free markets, milk whey from a local industrial disposal, and their combination, and Hestrin and Schramm media was used as standard culture media. Although all culture media studied produced BC, the highest BC yield-60 mg/mL-was achieved with the rotten fruit culture. Thus, the results showed that rotten fruit can be used for BC production. This culture media can be considered as a profitable alternative to generate high-value products. In addition, it combines environmental concern with sustainable processes that can promote also the reduction of production cost.

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Section: Introductionmentioning

confidence: 99%

Bacterial cellulose production by Gluconacetobacter xylinus by employing alternative culture media

Jozala

Pértile

Santos

et al. 2014

Appl Microbiol Biotechnol

159

View full text Add to dashboard Cite

show abstract

“…Bacterial cellulose (BC) is a microorganism-based homopolymer of β-(1,4) glucose with the formula ((C 6 H 10 O 5 ) n ) produced by various strains of the g e n e r a K o m a g a t a e i b a c t e r ( f o r m e r l y k n o w n a s Gluconacetobacter, Acetobacter), Agrobacterium, Aerobacter, Achromobacter, Alcaligenes, Azotobacter, Rhizobium, Pseudomonas, Sarcina, Salmonella, etc. (Li et al 2012;Huang et al 2014).…”

Section: Introductionmentioning

confidence: 97%

“…The biosynthesis of BC is a biochemical network containing a large number of key enzymes, and the regulation of the enzymes controls the production of cellulose (Ha et al 2011;Li et al 2012;Huang et al 2014). In addition to this, style of fermentation affects BC production.…”

Section: Introductionmentioning

confidence: 99%

Bacterial cellulose production by Komagataeibacter hansenii using algae-based glucose

Uzyol

Saçan

2016

Environ Sci Pollut Res

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Bacterial cellulose (BC) is a homopolymer and it is distinguished from plant-based cellulose by its unique properties such as high purity, high crystallinity, high water-holding capacity, and good biocompatibility. Microalgae are unicellular, photosynthetic microorganisms and are known to have high protein, starch, and oil content. In this study, Chlorella vulgaris was evaluated as source of glucose for the production of BC. To increase the starch content of algae the effect of nutrient starvation (nitrogen and sulfur) and light deficiency were tested in a batch assay. The starch contents (%) were 5.27 ± 0.04, 7.14 ± 0.18, 5.00 ± 0.08, and 1.35 ± 0.04 for normal cultivation, nitrogen starvation, sulfur starvation, and dark cultivation conditions, respectively. The performance of enzymatic and acidic methods was compared for the starch hydrolysis. This study demonstrated for the first time that acid hydrolysate of algal starch can be used to substitute glucose in the fermentation medium of Komagataeibacter hansenii for BC production. Glucose was used as a control for BC production. BC production yields on dry weight basis were 1.104 ± 0.002 g/L and 1.202 ± 0.005 g/L from algae-based glucose and glucose, respectively. The characterization of both BCs produced from glucose and algae-based glucose was investigated by scanning electron microscopy and Fourier transform infrared spectroscopy. The results have shown that the structural characteristics of algae-based BC were comparable to those of glucose-based BC.

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“…Till now, increasing BC yield is still a big challenge which limits its further application in various fields [10]. Bae and Shoda [11] reported that adding molasses at a lower concentration of 20 g/L into the cultivation medium improved the efficiency and the econometrics.…”

Section: Introductionmentioning

confidence: 99%

Revealing Differences in Metabolic Flux Distributions between a Mutant Strain and Its Parent Strain Gluconacetobacter xylinus CGMCC 2955

Zhong

Liu

et al. 2014

PLoS ONE

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A better understanding of metabolic fluxes is important for manipulating microbial metabolism toward desired end products, or away from undesirable by-products. A mutant strain, Gluconacetobacter xylinus AX2-16, was obtained by combined chemical mutation of the parent strain (G. xylinus CGMCC 2955) using DEC (diethyl sulfate) and LiCl. The highest bacterial cellulose production for this mutant was obtained at about 11.75 g/L, which was an increase of 62% compared with that by the parent strain. In contrast, gluconic acid (the main byproduct) concentration was only 5.71 g/L for mutant strain, which was 55.7% lower than that of parent strain. Metabolic flux analysis indicated that 40.1% of the carbon source was transformed to bacterial cellulose in mutant strain, compared with 24.2% for parent strain. Only 32.7% and 4.0% of the carbon source were converted into gluconic acid and acetic acid in mutant strain, compared with 58.5% and 9.5% of that in parent strain. In addition, a higher flux of tricarboxylic acid (TCA) cycle was obtained in mutant strain (57.0%) compared with parent strain (17.0%). It was also indicated from the flux analysis that more ATP was produced in mutant strain from pentose phosphate pathway (PPP) and TCA cycle. The enzymatic activity of succinate dehydrogenase (SDH), which is one of the key enzymes in TCA cycle, was 1.65-fold higher in mutant strain than that in parent strain at the end of culture. It was further validated by the measurement of ATPase that 3.53–6.41 fold higher enzymatic activity was obtained from mutant strain compared with parent strain.

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Improvement of bacterial cellulose production by manipulating the metabolic pathways in which ethanol and sodium citrate involved

Cited by 81 publications

References 37 publications

Bacterial cellulose production by Gluconacetobacter xylinus by employing alternative culture media

Bacterial cellulose production by Gluconacetobacter xylinus by employing alternative culture media

Bacterial cellulose production by Komagataeibacter hansenii using algae-based glucose

Revealing Differences in Metabolic Flux Distributions between a Mutant Strain and Its Parent Strain Gluconacetobacter xylinus CGMCC 2955

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