Improvement of Astragalin on Spermatogenesis in Oligoasthenozoospermia Mouse Induced by Cyclophosphamide

Fan, Qigang; Zhao, Zhongying; Meng, Qingguo; He, Rong; Liu, Hongli; Zhang, Meigui; Gao, Pu; Li, Xinlong; Li, Yang; Zhu, Qinying; Shen, Fengqin; Zhao, Lihui; Liang, Xiaolei

doi:10.1007/s43032-021-00808-8

Cited by 11 publications

(5 citation statements)

References 51 publications

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“…6 ). The same applies to the RPS6KA5 proliferation-promoting gene 32 , which in our study showed increased expression levels in the patient with a mutation in the ESX1 gene and decreased expression in TCam-2 cells with an activated ESX1 gene. Here, it also seems that under normal conditions, the ESX1 gene potentially regulates the expression level of the RPS6KA5 gene and consequently inhibits cell proliferation, leading to the differentiation process.…”

Section: Discussionsupporting

confidence: 76%

ESX1 gene as a potential candidate responsible for male infertility in nonobstructive azoospermia

Malcher,

Graczyk,

Bauer

et al. 2023

Sci Rep

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Infertility is a problem that affects approximately 15% of couples, and male infertility is responsible for 40–50% of these cases. The cause of male infertility is still poorly diagnosed and treated. One of the prominent causes of male infertility is disturbed spermatogenesis, which can lead to nonobstructive azoospermia (NOA). Whole-genome sequencing (WGS) allows us to identify novel rare variants in potentially NOA-associated genes, among others, in the ESX1 gene. The aim of this study was to activate the ESX1 gene using CRISPRa technology in human germ cells (testicular seminoma cells—TCam-2). Successful activation of the ESX1 gene in TCam-2 cells using the CRISPRa system was achieved, and the expression level of the ESX1 gene was significantly higher in modified TCam-2 cells than in WT cells or the negative control with nontargeted gRNA (p < 0.01). Using RNA-seq, a network of over 50 genes potentially regulated by the ESX1 gene was determined. Finally, 6 genes, NANOG, CXCR4, RPS6KA5, CCND1, PDE1C, and LINC00662, participating in cell proliferation and differentiation were verified in azoospermic patients with and without a mutation in the ESX1 gene as well as in men with normal spermatogenesis, where inverse correlations in the expression levels of the observed genes were noted.

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Section: Discussionsupporting

confidence: 76%

ESX1 gene as a potential candidate responsible for male infertility in nonobstructive azoospermia

Malcher,

Graczyk,

Bauer

et al. 2023

Sci Rep

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“…We noticed in the present study that CP signi cantly decreased testosterone and mRNA levels of StAR in the TM3 cells compared to control. Our ndings are consistent with those ofFan et al (2022) who reported that CP down regulated gene expression of genes related to spermatogenesis and lowered testosterone production by altering STAR, CYP11A1, and PRKACB genes. Of great interest, AgNPs elevated testosterone level and up regulated StAR genes after application.…”

supporting

confidence: 92%

Biosynthesis of silver nanoparticles using Pterorhachis zenkeri: Characterization and evaluation of antioxidant, anti-apoptotic, and androgenic properties in TM3 Leydig cells exposed to cyclophosphamide

Deeh,

Natesh,

Alagarsamy

et al. 2024

Preprint

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We fabricated silver nanoparticles (AgNPs) using Pterorhachis zenkeri, characterized by Atomic Force Microscopy (AFM) and determined their antioxidant potentials in vitro. Results confirmed the fabrication of AgNPs by using P. zenkeri as a bioreducing agent for the first time. AgNPs possessed potent antioxidant activity in vitro. Furthermore, the TM3 cells were treated for 24 h with AgNPs, vitamin E and cyclophosphamide (CP) at different concentrations (25, 50, 100, 250 and 500 µg/ml). The cells morphology, apoptosis, mitochondrial membrane depolarisation (MMD), Reactive Oxygen Species (ROS), caspase 3/9, oxidative stress-related enzymes, testosterone, and the mRNAexpression of steroidogenic acute regulatory protein (StAR) were measured. Results revealed that AgNPs, vitamin E and CP decreased the cell viability in a dose-dependent manner, but did not affect the TM3 cells morphology after treatment. The cytotoxicity of CP in TM3 cells was alleviated after AgNPs application. For instance, AgNPs significantly (p<0.001-0.05) reduced the MMD, ROS production, and caspase 3/9 activities, but increased the activities of superoxide dismutase, catalase and glutathione peroxidase in the TM3 cells. Moreover, AgNPs improved testosterone production by activating StAR machineries. These results indicate that AgNPs/P. zenkeri could be a potential alternative drug in the management of oxidative stress and androgen deficit associated with CP chemotherapy.

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“…Huatan Qushi decoction rescues oligoasthenozoospermia through the improvement of lipid metabolism and semen quality in obese rats ( Dong et al, 2019 ). Astragalin, one of the main active ingredients in TCM, alleviates oligoasthenozoospermia by increasing testosterone levels in the testis ( Fan et al, 2022 ). Previously, Gu et al reported the stimulative effect of YC on testosterone synthesis via the Nur77 pathway in Leydig cells ( Gu et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Yangjing capsule improves oligoasthenozoospermia by promoting nitric oxide production through PLCγ1/AKT/eNOS pathway

et al. 2023

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Background: Oligoasthenozoospermia is an important factor leading to male infertility. Yangjing capsule (YC), a traditional Chinese preparation, displays beneficial effects on male infertility. However, whether YC could improve oligoasthenozoospermia remains unclear.Methods: In this study, we aimed to explore the effect of YC in the treatment of oligoasthenozoospermia. Male Sprague-Dawley (SD) rats were treated with 800 mg/kg ornidazole once daily for 30 days to induce in vivo oligoasthenozoospermia; primary Sertoli cells were treated with 400 μg/mL ornidazole for 24 h to induce in vitro oligoasthenozoospermia.Results: We found that YC improved the testicle and epididymis weight, sperm concentration, sperm progressive motility, serum testosterone, fertility rate and testis morphology in ornidazole-exposed rats and enhanced cell survival in ornidazole-stimulated primary Sertoli cells. YC also inhibited the ornidazole-caused decrease in nitric oxide (NO) generation and the phosphorylation of phospholipase C γ1 (PLCγ1), AKT, and eNOS in vivo and in vitro in oligoasthenozoospermia. Furthermore, the knockdown of PLCγ1 blunted the beneficial effects of YC in vitro.Conclusion: Collectively, our data suggested that YC protected against oligoasthenozoospermia by promoting NO levels through the PLCγ1/AKT/eNOS pathway.

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Improvement of Astragalin on Spermatogenesis in Oligoasthenozoospermia Mouse Induced by Cyclophosphamide

Cited by 11 publications

References 51 publications

ESX1 gene as a potential candidate responsible for male infertility in nonobstructive azoospermia

ESX1 gene as a potential candidate responsible for male infertility in nonobstructive azoospermia

Biosynthesis of silver nanoparticles using Pterorhachis zenkeri: Characterization and evaluation of antioxidant, anti-apoptotic, and androgenic properties in TM3 Leydig cells exposed to cyclophosphamide

Yangjing capsule improves oligoasthenozoospermia by promoting nitric oxide production through PLCγ1/AKT/eNOS pathway

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