Improvement of antioxidant potential in rats consuming feathers protein hydrolysate obtained by fermentation of the keratinolytic bacterium, Bacillus pumilus A1

Abstract

The in vitro and in vivo antioxidant activities of feathers protein hydrolysate (FPH), produced by fermentation with the keratinolytic bacterium, Bacillus pumilus strain A1, were evaluated. The antioxidant activities of FPH, evaluated using DNA nicking and ferrozine assays, demonstrate that FPH present an important antioxidant potential. Interestingly, the addition of this hydrolysate (2.5 and 5%) to the feed of rats caused a significant decrease in the rate of thiobarbutiric acid reagent substances (TBARS) and the enzymatic antioxidants activities (superoxide dismutase, glutathione peroxidase and catalase) in liver, kidney, heart, brain and cerebella, as compared to the control. However, the supplementation of the standard food by 2.5% of untreated feather meal or 5% of soya is associated with an increase of the TBARS rate and enzymatic antioxidant activities. These results indicate that FPH may be useful as supplementary proteins and natural antioxidant in animal feed formulation.

Hydrothermal–enzymatic process for the bio‐valorization of keratin wastes by thermostable keratinase from Thermoactinomyces vulgarisTK1‐21

“…Kr6, 57 Bacillus subtilis S1-4, 48 and Bacillus pumilus A1. 58 From the environmental perspective, the hydrothermal-enzymatic hydrolysis developed in this study could be considered as a clean production process because of nontoxic chemical utilization and the mild conditions. 59 Therefore, the developed hydrothermalenzymatic hydrolysis could be an alternative process under economic and eco-friendly considerations for KH production from keratin wastes.…”

“…The satisfactory antioxidant activity of KH was also observed when using keratinase from Chryseobacterium sp. Kr6, 57 Bacillus subtilis S1‐4, 48 and Bacillus pumilus A1 58 …”

Wool keratin as a novel alternative protein: A comprehensive review of extraction, purification, nutrition, safety, and food applications

“…The digestibility and health improvement potential of keratin hydrolysates have been tested on rodents, both rats and mice (Dias et al., 2022; Nahed et al., 2012; Oluba et al., 2019), cats (Deb‐Choudhury et al., 2018), chicken (Alabi et al., 2021), and humans (Crum et al., 2018). Wool keratin supplementation in feeds can positively influence the growth performance parameters (fed conversion efficiency, weight gain, weight of liver, kidney, and heart) of various animal models.…”