Improvement of a capillary electrophoresis/frontal analysis (CE/FA) method for determining binding constants: Discussion on relevant parameters

Vuignier, Karine; Schappler, Julie; Veuthey, Jean‐Luc; Carrupt, Pierre‐Alain; Martel, Sophie

doi:10.1016/j.jpba.2010.07.024

Cited by 39 publications

(30 citation statements)

References 55 publications

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“…Numerous parameters must be taken into consideration for a successful application of CE–FA . On the other hand, several of them were strictly determined by the nature of this study that should approximate the physiological conditions in human blood.…”

Section: Resultsmentioning

confidence: 99%

Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis‐frontal analysis

Michalcová

Glatz

2016

J of Separation Science

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Diabetes is one of the most widespread diseases characterized by a deficiency in the production of insulin or its ineffectiveness. As a result, the increased concentrations of glucose in the blood lead not only to damage to many of the body's systems but also cause the nonenzymatic glycation of plasma proteins affecting their drug binding. Since the binding ability influences its pharmacokinetics and pharmacodynamics, this is a very important issue in the development of new drugs and personalized medicine. In this study, capillary electrophoresis-frontal analysis was used to evaluate the affinities between human serum albumin or its glycated form and the first generation of sulfonylurea antidiabetics, since their inadequate concentration may induce hypoglycaemia or on the contrary hyperglycaemia. The binding constants decrease in the sequence acetohexamide > tolbutamide > chlorpropamide > carbutamide both for normal and glycated human serum albumins, with glycated giving lower values. These results provide a more quantitative picture of how these drugs bind with normal and modified human serum albumin and indicate capillary electrophoresis-frontal analysis to be another tool for examining the changes arising from modifications of albumin, or any other protein, with all its benefits like short analysis time, small sample requirement, and automation.

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Section: Resultsmentioning

confidence: 99%

Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis‐frontal analysis

Michalcová

Glatz

2016

J of Separation Science

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“…It is required that the mobility of drug differs from that of protein or the drug-protein complex for the interaction study using CE-FA [11,18]. At near physiological conditions (67 mM phosphate buffer, pH 7.4, I = 0.17, 37 • C), the electrophoretic mobility of captopril, hydrochlorothiazide, and HSA were determined respectively, by injecting the corresponding working solution into the capillary filled with BGE.…”

Section: Ce-fa Methods Developmentmentioning

confidence: 99%

“…Capillary electrophoresis (CE) has been proved to be more convenient and cost-effective for the interaction study [5,[12][13][14][15][16][17]. There are several modes of CE have been proposed for the quantitative assessment of drug-protein interactions, such as affinity capillary electrophoresis, the Hummel-Dreyer method, vacancy peak method, vacancy affinity capillary electrophoresis, and capillary electrophoresis-frontal analysis (CE-FA) [11,18]. CE advantages, such as high efficiency and separation selectivity, low sample and reagent consumption, and ability to work under physiological relevant conditions with the specific requirements for pH, ionic strength and temperature [19].…”

Section: Introductionmentioning

confidence: 99%

Application of capillary electrophoresis-frontal analysis for comparative evaluation of the binding interaction of captopril with human serum albumin in the absence and presence of hydrochlorothiazide

Liu

Xiang

Wang

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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“…Herein we present an evaluation of the binding properties and selectivity of histamine‐imprinted microspheres (MIMs) by frontal analysis capillary electrophoresis (FACE). While the FACE method has been widely used as a tool to study binding interactions (non‐covalent) of protein with drugs and polyelectrolytes, the method has not been applied to MIP systems.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the binding performance of histamine‐imprinted microspheres by frontal analysis capillary electrophoresis

et al. 2017

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Frontal analysis capillary electrophoresis was used to evaluate the binding performance of molecularly imprinted microspheres (MIM) toward its template histamine and analogs at pH 7, and compared to the high performance liquid chromatographic method. In both methods, batch binding was employed and the binding parameters were calculated from the measured concentration of unbound amine analytes and afforded comparable histamine equilibrium dissociation constants (K ∼ 0.4 mM). FACE was easily carried out at shorter binding equilibration time (i.e. 30 min) and without the need to separate the microspheres, circumventing laborious and, in the case of the system under study, inefficient sample filtration. It also allowed for competitive binding studies by virtue of its ability to distinctly separate intact microspheres and all tested amines which could not be resolved in HPLC. K 's for nonimprinted (control) microspheres (NIM) from FACE and HPLC were also comparable (∼ 0.6 mM) but at higher histamine concentrations, HPLC gave lower histamine binding. This discrepancy was attributed to inefficient filtration of the batch binding samples prior to HPLC analysis resulting in an over-estimation of the concentration of free histamine brought about by the presence of unfiltered histamine-bound microspheres.

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Improvement of a capillary electrophoresis/frontal analysis (CE/FA) method for determining binding constants: Discussion on relevant parameters

Cited by 39 publications

References 55 publications

Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis‐frontal analysis

Study on the interactions of sulfonylurea antidiabetic drugs with normal and glycated human serum albumin by capillary electrophoresis‐frontal analysis

Application of capillary electrophoresis-frontal analysis for comparative evaluation of the binding interaction of captopril with human serum albumin in the absence and presence of hydrochlorothiazide

Assessment of the binding performance of histamine‐imprinted microspheres by frontal analysis capillary electrophoresis

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