Improvement in the effectiveness of Cryptococcus laurentii to control postharvest blue mold of pear by its culture in β-glucan amended nutrient broth

Zeng, Lizhen; Chen, Yu; Fu, Da; Lu, Huangping; Zhu, Ruiyu; Lu, Laifeng; Zheng, Xiaodong; Yu, Ting

doi:10.1016/j.postharvbio.2015.03.005

Cited by 14 publications

(6 citation statements)

References 39 publications

(49 reference statements)

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“…Therefore, it is very important to enhance the biocontrol efficacy of antagonistic yeasts. It has been reported that β-glucan could improve the biocontrol efficacy of C. laurentii and C. podzolicus against postharvest blue mold of pears [24]. In the present study, our results also confirmed that β-glucan was an effective elicitor to improve the biocontrol ability of S. spartinae W9 on gray mold of strawberry fruit.…”

Section: Discussionsupporting

confidence: 88%

“…According to the result of an extracellular hydrolase assay plate, we determined the GLU activity of S. spartinae W9. S. spartinae W9 cultured in NYDB or NYDB with 0.1% βglucan were sampled at 12,16,20,24,36, and 48 h. The fermentation broth was centrifuged at 6000 rpm for 10 min, and the supernatant was filtered through a sterile membrane for enzyme assay. The GLU activity was determined using the 3, 5-dinitrosalicylic acid method [22], and the absorbance at 540 nm was measured to calculate GLU activity.…”

Section: Measurement Of Extracellular Hydrolasesmentioning

confidence: 99%

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β-Glucan Enhances the Biocontrol Efficacy of Marine Yeast Scheffersomyeces spartinae W9 against Botrytis cinerea in Strawberries

Chen

Wei

Zou

et al. 2023

JoF

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The marine yeast Scheffersomyeces spartinae W9 is a promising biocontrol agent for gray mold caused by Botrytis cinerea in strawberries. Improving the biocontrol efficacy of S. spartinae W9 is necessary for its commercial application. In this study, different concentrations of β-glucan were added to the culture medium to evaluate its effect on the biocontrol efficacy of S. spartinae W9. The results showed that 0.1% β-glucan could increase the biocontrol effect of S. spartinae W9 against B. cinerea in strawberries and in vitro. We found that adding 0.1% β-glucan to the culture medium promoted the growth of S. spartinae W9 in wounds of strawberries, enhanced biofilm formation ability, and secreted more β-1,3-glucanase. In addition, 0.1% β-glucan increased the survival rate of S. spartinae W9 under oxidative, thermal, osmotic, and plasma membrane stressors. Transcriptome analysis revealed 188 differential expressed genes in S. spartinae W9 cultured with or without 0.1% β-glucan, including 120 upregulated and 68 downregulated genes. The upregulated genes were associated with stress response, cell wall formation, energy production, growth, and reproduction. Thus, culturing with 0.1% β-glucan is an effective way to improve the biocontrol ability of S. spartinae W9 against gray mold in strawberries.

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Section: Discussionsupporting

confidence: 88%

Section: Measurement Of Extracellular Hydrolasesmentioning

confidence: 99%

β-Glucan Enhances the Biocontrol Efficacy of Marine Yeast Scheffersomyeces spartinae W9 against Botrytis cinerea in Strawberries

Chen

Wei

Zou

et al. 2023

JoF

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“…Catalase activity was determined as specified in Zeng et al . (). 200 μL H 2 O 2 (30 m m ) in phosphate buffer (50 m m , pH 7) was added to 50 μL crude enzyme solution.…”

Section: Methodsmentioning

confidence: 99%

“…Superoxide dismutase (SOD) activity was determined per the protocol published by Zeng et al . (), based on the photochemical reduction in nitro blue tetrazolium (NBT). 3.7 mL NBT reagent (50 m m phosphate buffer [pH 7.8] with 77.12 μ m nitroblue tetrazolium (NBT), 100 μ m EDTA‐Na 2 and 13.37 m m methionine solution) was added to 1 mL crude enzyme solution, then warmed the mixture at 30 °C for 2 min.…”

Section: Methodsmentioning

confidence: 99%

The effect and mechanism of ultrasonic treatment on the postharvest texture of shiitake mushrooms (Lentinula edodes)

Ying

2018

Int J of Food Sci Tech

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Summary Fresh shiitake mushrooms (Lentinula edodes) are prone to texture softening and quality decline during storage. We investigated the effects of prestorage ultrasonic treatment on the textural changes of the mushrooms during storage. We monitored physiological parameters, such as respiration intensity, weight loss, cell wall composition and resistance‐related enzymes activity. The hardness of the ultrasonic‐treated (40 kHz, 80 W L−1, 20 min) mushrooms after 12 days of storage (0.461 N) was significantly higher than the untreated control group (0.286 N). The weight loss and respiration intensity of the ultrasonic‐treated group were significantly lower, whereas the cell wall contents, including glucan and chitin, were significantly higher. The ultrasonic‐treated mushrooms also had higher phenylalanine ammonia lyase, superoxide dismutase and catalase activities. Thus, ultrasound preserves the texture of mushrooms by reducing metabolic rate, maintaining cell wall compositions, activating the activity of SOD and maintaining the activity of PAL and CAT.

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“…The main antagonistic mechanisms of the bacteria and yeasts in vitro against the phytopathogens are the production of hydrolytic enzymes, such as β-1,3-glucanase [38,39], which break the links of the glucan present in the cell wall of the phytopathogen, limiting their germination and growth. This enzyme has already been reported for B. subtilis [40], S. rhizophila [30,41,42], and C. laurentii [43]. The competence for space and nutrients is another mechanism through which the bacteria and yeasts limit phytopathogens in vitro because these microorganisms have a short period of exponential growth that allows them to exhaust carbon sources in their culture medium, thereby minimizing zoospore germination and phytopathogen growth [44,45].…”

Section: Discussionmentioning

confidence: 71%

Efficiency of Marine Bacteria and Yeasts on the Biocontrol Activity of Pythium ultimum in Ancho-Type Pepper Seedlings

et al. 2020

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Ancho-type pepper (Capsicum annuum L.) is a crop susceptible to Pythium ultimum, which has already been controlled with synthetic fungicide applications; however, marine antagonist microorganisms could be an alternative source of control. The efficiency in vitro and in vivo of marine bacteria and yeasts was determined against P. ultimum. The inhibition of the radial growth of P. ultimum was quantified in vitro by the bacteria Stenotrophomonas rhizophila KM01 and KM02; Bacillus subtilis RBM01 and RBM02, B. amyloliquefaciens 2RLBF and 3R4CF; and Pseudomonas spp. 2R6BF and 2RE9CF, as well as the yeasts Debaryomyces hansenii 1R11AB, 1R11CB, and LL01 and Cryptococcus laurentii 2R3BF and 2R1CB. The β-1,3-glucanase activity of the marine microorganisms was quantified in the presence of the phytopathogen. The disease index (DI), growth parameters, and colony forming units (CFU) were determined in ancho-type pepper plants inoculated with marine bacteria, yeasts, and P. ultimum. The radial zone of the phytopathogen was inhibited by 80% and 75% by S. rhizophila KM01 and C. laurentii 2R1CB, respectively. D. hansenii LL01 and S. rhizophila KM02 showed the highest activity of β-1,3-glucanase, with 6060 U/mL and 47 U/mL, respectively. B. subtilis RBM02 protected 100% of the plants from the oomycete, and an increase was quantified in all the growth parameters and CFU. The use of these marine bacteria and yeasts are, therefore, an option for P. ultimum biocontrol in ancho-type pepper plants, thereby minimizing the application of synthetic fungicides.Agronomy 2020, 10, 408 2 of 12 by immediate infection makes management of this phytopathogen very difficult [7]. Several Pythium species, including P. aphanidermatum, P. irregular, and P. ultimum, are known to cause damping-off and root rot diseases in pepper [8][9][10]. Although synthetic fungicides have shown promising results in controlling damping-off disease, phytotoxicity and fungicide residues pose serious problems to human and animal health, as well as the environment. In this context, phytosanitary measures and the management of pepper cultivation should include the application of microorganisms as biocontrol agents to minimize the use of synthetic fungicides [11].Bacteria and yeasts have a high capacity to control phytopathogens without causing damage to human and animal health and the environment [12,13]. Several mechanisms have been proposed for the biocontrol of phytopathogens by bacteria and yeasts, including competition for space and nutrients [14,15], lytic enzyme production such as β-1,3-glucanase [16,17], and induction of host resistance [18,19], among others. Both microorganisms have been isolated in different terrestrial ecosystems, mainly in plants, fruits, and soils; the main bacteria used as biocontrol agents are species of Stenotrophomonas, Bacillus, and Pseudomonas, which have already been reported for the control of Fusarium proliferatum in melon [20]; Sclerotium rolfsii [21] and Rhizoctonia solani in wheat [22]; and F. solani in cassava [23], among others. O...

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Improvement in the effectiveness of Cryptococcus laurentii to control postharvest blue mold of pear by its culture in β-glucan amended nutrient broth

Cited by 14 publications

References 39 publications

β-Glucan Enhances the Biocontrol Efficacy of Marine Yeast Scheffersomyeces spartinae W9 against Botrytis cinerea in Strawberries

β-Glucan Enhances the Biocontrol Efficacy of Marine Yeast Scheffersomyeces spartinae W9 against Botrytis cinerea in Strawberries

The effect and mechanism of ultrasonic treatment on the postharvest texture of shiitake mushrooms (Lentinula edodes)

Efficiency of Marine Bacteria and Yeasts on the Biocontrol Activity of Pythium ultimum in Ancho-Type Pepper Seedlings

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