Improvement in salt-tolerance of Aspergillus oryzae γ-glutamyl transpeptidase via protein chimerization with Aspergillus sydowii homolog

Senba, Hironori; Nishikawa, Arisa; Kimura, Yukihiro; Tanaka, Shin‐ichi; Matsumoto, Junichi; Doi, Mikiharu; Takenaka, Shinji

doi:10.1016/j.enzmictec.2023.110240

Cited by 2 publications

(2 citation statements)

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“…Glutamyl transpeptidase is another pivotal enzyme involved in glutamate production during high salt fermentation of soy sauce by A. oryzae . The gamma-glutamyl transpeptidase derived from Aspergillus sydowii exhibits high activity even under high-salt conditions (Senba et al 2023 ). It has also been shown to increase the levels of the fresh flavor amino acid L-glutamate in soy sauce.…”

Section: Examples Of Microbial-derived Stps Applied To High-salt Trad...mentioning

confidence: 99%

“…The catalytic mechanisms of STPs have been intensively studied using molecular biology and protein chemistry techniques to reveal their unique catalytic properties in high-salt environments (Gao et al 2019 ). Using protein modification and computer-aided simulation techniques, researchers have successfully modified protease molecules to enhance the salt tolerance of the enzyme itself (Senba et al 2023 ; Takenaka et al 2022 ; Takita et al 2008 ). Novel STP preparations have been developed and are being tested for applications in biopharmaceuticals, food processing, environmental treatment, and other fields (Fu et al 2008 ; Hao et al 2022 ; Zhang et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Microbial-derived salt-tolerant proteases and their applications in high-salt traditional soybean fermented foods: a review

Yao,

Liu,

Liu

et al. 2023

Bioresour. Bioprocess.

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Different microorganisms can produce different proteases, which can adapt to different industrial requirements such as pH, temperature, and pressure. Salt-tolerant proteases (STPs) from microorganisms exhibit higher salt tolerance, wider adaptability, and more efficient catalytic ability under extreme conditions compared to conventional proteases. These unique enzymes hold great promise for applications in various industries including food, medicine, environmental protection, agriculture, detergents, dyes, and others. Scientific studies on microbial-derived STPs have been widely reported, but there has been little systematic review of microbial-derived STPs and their application in high-salt conventional soybean fermentable foods. This review presents the STP-producing microbial species and their selection methods, and summarizes and analyzes the salt tolerance mechanisms of the microorganisms. It also outlines various techniques for the isolation and purification of STPs from microorganisms and discusses the salt tolerance mechanisms of STPs. Furthermore, this review demonstrates the contribution of modern biotechnology in the screening of novel microbial-derived STPs and their improvement in salt tolerance. It highlights the potential applications and commercial value of salt-tolerant microorganisms and STPs in high-salt traditional soy fermented foods. The review ends with concluding remarks on the challenges and future directions for microbial-derived STPs. This review provides valuable insights into the separation, purification, performance enhancement, and application of microbial-derived STPs in traditional fermented foods. Graphical Abstract

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Section: Examples Of Microbial-derived Stps Applied To High-salt Trad...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microbial-derived salt-tolerant proteases and their applications in high-salt traditional soybean fermented foods: a review

Yao,

Liu,

Liu

et al. 2023

Bioresour. Bioprocess.

View full text Add to dashboard Cite

show abstract

Heterologous expression and characterization of salt-tolerant β-glucosidase from xerophilic Aspergillus chevalieri for hydrolysis of marine biomass

Senba,

Saito,

Kimura

et al. 2023

Arch Microbiol

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Improvement in salt-tolerance of Aspergillus oryzae γ-glutamyl transpeptidase via protein chimerization with Aspergillus sydowii homolog

Cited by 2 publications

References 50 publications

Microbial-derived salt-tolerant proteases and their applications in high-salt traditional soybean fermented foods: a review

Microbial-derived salt-tolerant proteases and their applications in high-salt traditional soybean fermented foods: a review

Heterologous expression and characterization of salt-tolerant β-glucosidase from xerophilic Aspergillus chevalieri for hydrolysis of marine biomass

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