Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis

Oliveira, Arthur Maynart Pereira; Neto, Pedro de Oliva

doi:10.1590/s1516-89132011000500019

Cited by 11 publications

(10 citation statements)

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“…While yeast extract can have a diverse composition, assuming a composition and then estimating the impact on the optimization still allows to explore the boundaries of the system and to get an estimate on the feasibility of the measured rates. We assumed that the yeast extract would consist of around 58% protein (proteins, peptides, amino acids), and 14.2% RNA about 2.5% carbohydrates and the balance ashes, using the known precursor demand to synthesize the macromolecules in baker’s yeast [32], we estimate total available precursors (assuming the cells can use all components). The analysis is to be seen as a tool to estimate the overall feasibility of the analysed dataset.…”

Section: Methodsmentioning

confidence: 99%

Redox dependent metabolic shift in Clostridium autoethanogenum by extracellular electron supply

Kracke

Virdis

Bernhardt

et al. 2016

Biotechnol Biofuels

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BackgroundMicrobial electrosynthesis is a novel approach that aims at shifting the cellular metabolism towards electron-dense target products by extracellular electron supply. Many organisms including several acetogenic bacteria have been shown to be able to consume electrical current. However, suitable hosts for relevant industrial processes are yet to be discovered, and major knowledge gaps about the underlying fundamental processes still remain.ResultsIn this paper, we present the first report of electron uptake by the Gram-positive, ethanol-producing acetogen, Clostridium autoethanogenum. Under heterotrophic conditions, extracellular electron supply induced a significant metabolic shift away from acetate. In electrically enhanced fermentations on fructose, acetate production was cut by more than half, while production of lactate and 2,3-butanediol increased by 35-fold and threefold, respectively. The use of mediators with different redox potential revealed a direct dependency of the metabolic effect on the redox potential at which electrons are supplied. Only electrons delivered at a redox potential low enough to reduce ferredoxin caused the reported effect.ConclusionsProduction in acetogenic organisms is usually challenged by cellular energy limitations if the target product does not lead to a net energy gain as in the case of acetate. The presented results demonstrate a significant shift of carbon fluxes away from acetate towards the products, lactate and 2,3-butanediol, induced by small electricity input (~0.09 mol of electrons per mol of substrate). This presents a simple and attractive method to optimize acetogenic fermentations for production of chemicals and fuels using electrochemical techniques. The relationship between metabolic shift and redox potential of electron feed gives an indication of possible electron-transfer mechanisms and helps to prioritize further research efforts.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0663-2) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Redox dependent metabolic shift in Clostridium autoethanogenum by extracellular electron supply

Kracke

Virdis

Bernhardt

et al. 2016

Biotechnol Biofuels

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“…Yeast cells are reported to undergo rapid autolysis upon heating under acidic conditions as compared to osmotic shock-induced autolysis, 16 which not only takes a longer time, but also requires continuous shaking at elevated temperature (60 to 90 ∘ C) for complete autolysis. Therefore, this property was utilized to eliminate the need for separate autolysis of spent yeast by osmotic shock, resulting in significant cost-and energy-saving in media preparation.…”

Section: Effect Of Media Optimization On Valorization Of Waste Streammentioning

confidence: 99%

Development of continuous cultivation process for oil production through bioconversion of minimally treated waste streams from second‐generation bioethanol production

Singh

Mehta

Saxena

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Efficient conversion of inhibitor containing pentose rich stream into bioethanol or biogas is still challenging due to several technological challenges; however, this stream can be efficiently assimilated into lipids by oleaginous yeasts such as Rhodotorula. In this work, a continuous and nonsterile process was developed for bioconversion of waste streams into lipids for biodiesel using growth‐inhibitor‐tolerant oleaginous yeast. RESULTS An inhibitor‐tolerating property of the strain was used to develop a cyclic and continuous near axenic cultivation process in a 2 L nonsterile bioreactor to enhance valorization of waste streams and to reduce energy requirements for media and vessel sterilization. After media optimization, continuous cultivation resulted in significant increase in biomass (14.90 g L−1 day−1) and lipid productivity (6.15 g L−1 day−1) as compared to flask (biomass ∼3 g L−1 day−1 and lipid productivity ∼0.6 g L−1 day−1) results. Palmitic acid (C16:0) and oleic acid (C18:1) were the major fatty acids, accounting for almost 80% of total lipids, thus making lipid produced in the process suitable for biodiesel production. CONCLUSION Inhibitors present in waste streams successfully maintained near axenic conditions during the cultivation. Maximum dilution rate of 1 was achieved after media optimization, which resulted in complete valorization of nutrients into biomass and lipids with high productivity. © 2018 Society of Chemical Industry

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“…Besides the diverse techniques employed to disrupt the microbial cell walls, there is another range of methodologies applied to remove the typically high content of nucleic acids in microbial biomass (Skrede et al 2009;Oliveira & Oliva Neto 2011). For example, yeast contains 6-15% of nucleic acids as compared with 2% in meat products (Arora et al 1991).…”

Section: Current and Future Challengesmentioning

confidence: 99%

Potential of microbial‐derived nutrients for aquaculture development

Gamboa‐Delgado

Márquez-Reyes

2016

Reviews in Aquaculture

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Fisheries and aquaculture products will have a lead role in satisfying the food requirements of the growing human population. The fish meal required for the manufacture of aquaculture feeds represents a limited resource having high demand. Unconventional sources of nutrients have been intensely researched and among these, the microbial biomass produced from heterotrophic and autotrophic microorganisms has been considered as a promising substitute for animal‐ and plant‐derived ingredients. Several studies have shown that different species of yeast, bacteria and microalgae are viable for high density culture and show excellent nutritional characteristics for terrestrial and aquatic animals. Such features have lead to additional research to upscale production and although production costs are still high; novel, sophisticated methods have focused on utilizing alternative substrates for producing microbial biomass. The aquaculture industry requires renewable nutrient sources that can yield high quality protein and other essential nutrients. In this context, the nutritional characteristics of microorganisms and the emerging technologies aimed to produce microbial biomass allow forecasting increased applications. The present manuscript reviews the current state of the production and use of microorganisms as dietary ingredients in aquaculture nutrition, with emphasis on those showing solid potential as functional additives and fish meal replacement ingredients. A synthesis of recent results on the effects promoted by the dietary inclusion of microbial biomass is presented, underlining the employment of different sources of yeast, bacteria and microalgae and the physiological ability of aquatic animals to utilize these alternative nutrient sources.

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Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis

Abstract: The optimization of autolysis of Saccharomyces cerevisiae from brewery was studied aiming at the maximum ribonucleic acid extraction and yeast extract production.

Cited by 11 publications

References 11 publications

Redox dependent metabolic shift in Clostridium autoethanogenum by extracellular electron supply

Redox dependent metabolic shift in Clostridium autoethanogenum by extracellular electron supply

Development of continuous cultivation process for oil production through bioconversion of minimally treated waste streams from second‐generation bioethanol production

Potential of microbial‐derived nutrients for aquaculture development

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