Improvement in non-programmable sperm cryopreservation technique in farmed greenlip abalone Haliotis laevigata

Liu, Yibing; Li, Xiaoxu; Xu, Tong; Robinson, Nick; Qin, Jianguang

doi:10.1016/j.aquaculture.2014.08.033

Cited by 14 publications

(14 citation statements)

References 18 publications

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“…Addition of non-permeable cryoprotectants to permeable cryoprotectants could provide better osmotic balance and cell membrane cohesiveness, and minimizes ice crystallization. This strategy has been widely used to improve cryopreserved sperm quality in different animal clades including mammals [1,15], teleosts [10,35] and invertebrates [2,26].…”

Section: Discussionmentioning

confidence: 99%

Improvement of post-thaw sperm survivals using liquid nitrogen vapor in a spermcasting oyster Ostrea angasi

Hassan

Qin

2017

Cryobiology

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Low survival of cryopreserved sperm impedes the application of cryopreservation technique in spermcasting oyster species. This study developed a simple method of liquid nitrogen vapor freezing to improve post-thaw sperm survival in the spermcasting oyster Ostrea angasi. The results indicate that the permeable cryoprotectants, dimethyl sulfoxide (DMSO), ethylene glycol (EG) and propylene glycol (PG) were non-toxic to sperm up to 20% concentration and 90 min exposure whereas methanol at 10% or higher was toxic to sperm for any exposure over 30 min. Among the treatments with permeable cryoprotectants, 15% EG produced the highest post-thaw sperm motility. Sperm motility was further improved by the addition of non-permeable cryoprotectants (trehalose and glucose), with 15% EG + 0.2 M trehalose resulting in the highest post-thaw sperm motility among all the combinations evaluated. The durations of 20, 30 and 60 min equilibrations produced a higher post-thaw sperm motility and plasma membrane integrity (PMI) than 10 min. Higher post-thaw motility and PMI were achieved by freezing sperm at the 8 cm height from the liquid nitrogen surface than at the 2, 4, 6, 10 or 12 cm height. Holding sperm for 10 min in liquid nitrogen vapor produced higher post-thaw motility and PMI than for 2, 5 or 20 min. The cryopreservation protocol developed in this study improved both post-thaw motility and PMI of O. angasi sperm at least 15% higher than those cryopreserved using programmable freezing method. Liquid nitrogen vapor freezing might have greater applicability in improving post-thaw sperm quality of spermcasting oyster species.

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Section: Discussionmentioning

confidence: 99%

Improvement of post-thaw sperm survivals using liquid nitrogen vapor in a spermcasting oyster Ostrea angasi

Hassan

Qin

2017

Cryobiology

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“…), scallops Argopecten purpuratus (Espinoza, Valdivia & Dupré ), farmed greenlip and black‐lip abalone (Liu, Li et al . ; Liu, Xu et al . , ) where glucose and trehalose have been found to enhance the post‐thaw sperm quality.…”

Section: Discussionmentioning

confidence: 99%

“…A similar result has also been reported in farmed greenlip abalone (Liu, Li et al . ) and striped bass Morone saxatilis (He & Woods ).…”

Section: Discussionmentioning

confidence: 99%

“…; Sánchez‐Lazo & Martínez‐Pita ,b). Gamete cryopreservation is one of the effective techniques that could achieve these goals and has been well advanced in Pacific oysters Crassostrea gigas (Adams, Smith, Roberts, Janke, King, Tervit & Webb ; Zhang, Li, Clarke & Li ) and abalone (Liu, Li, Xu, Robinson & Qin ; Zhu, Li, Qu, Liu & Clarke ). Sperm cryopreservation in blue mussels has been investigated by Matteo, Langellotti, Masullo and Sansone () using programmable freezing technique and sperm collected by natural spawning method, with a post‐thaw sperm fertilization rate of approximately 70% (relative to control) being achieved.…”

Section: Introductionmentioning

confidence: 99%

“…; Purdy ; Cabrita, Ma, Diogo, Martínez‐Páramo, Sarasquete & Dinis ; Liu, Li et al . , ; Liu, Xu et al . , ), and has been applied in rams (Jafaroghli, Khalili, Farshad & Zamiri ), boars (Gómez‐Fernández, Gómez‐Izquierdo, Tomás, Mocé & Mercado ), black‐lip pearl oysters (Lyons et al .…”

Section: Introductionmentioning

confidence: 99%

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Cryopreservation of strip spawned sperm using non-programmable freezing technique in the blue musselMytilus galloprovincialis

Liu

et al. 2015

Aquac Res

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In this study, a non-programmable freezing technique has been developed with the strip spawned blue mussel (Mytilus galloprovincialis) sperm. The key parameters optimized including (1) cryoprotectant agents (CPAs); (2) cooling temperature; (3) thawing temperature; (4) sugar and amino acid supplementation and (5) sperm to egg ratio. The fertilization rate and/or integrity of plasma membrane and acrosome were used as sperm quality assessment indicators. The highest post-thaw sperm fertilization rate of 95% was achieved, when sperm were cryopreservated in 8% dimethyl sulfoxide at 7.8 cm above the liquid nitrogen surface and thawed in a 60°C seawater bath. The addition of glucose, sucrose or trehalose in dimethyl sulfoxide did not, whereas 0.8% glycine did significantly improve the post-thaw sperm fertilization rates. The fluorescent evaluation has demonstrated that the addition of glycine significantly improved the post-thaw sperm acrosome integrity, revealing a positive role of glycine in the improvement of postthaw sperm quality in blue mussels.

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Cryopreservation of strip spawned sperm using programmable freezing technique in the blue mussel Mytilus galloprovincialis

Liu

et al. 2018

J. Ocean. Limnol.

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Improvement in non-programmable sperm cryopreservation technique in farmed greenlip abalone Haliotis laevigata

Cited by 14 publications

References 18 publications

Improvement of post-thaw sperm survivals using liquid nitrogen vapor in a spermcasting oyster Ostrea angasi

Improvement of post-thaw sperm survivals using liquid nitrogen vapor in a spermcasting oyster Ostrea angasi

Cryopreservation of strip spawned sperm using non-programmable freezing technique in the blue musselMytilus galloprovincialis

Cryopreservation of strip spawned sperm using programmable freezing technique in the blue mussel Mytilus galloprovincialis

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