Improvement in Menopause‐Associated Hepatic Lipid Metabolic Disorders by Herbal Formula HPC03 on Ovariectomized Rats

Abstract

Postmenopausal women have an increased risk of developing nonalcoholic fatty liver disease (NAFLD). We formulated a combination of three herb mixtures (HPC03) and observed lipid-lowering efficacy. HepG2 cells were treated with oleic acid to induce an NAFLD model (in vitro). Also, we investigated potential of HPC03 in an ovariectomize- (OVX-) induced NAFLD model (in vivo). We separated the mice into six groups, as follows: SHAM, OVX, OVX + β-estradiol, and OVX + HPC03 (50, 100, and 200 mg/kg). Rats were administered with/without HPC03 for 12 weeks. HPC03 dose dependently inhibited the lipid accumulation involved in lipogenesis in HepG2 cells. The body weight, fat mass, and weights of the liver were decreased in the OVX group than that in the other groups. HPC03 had decreased adiposity that was induced by OVX. HPC03 treatment reduced liver lipid deposition and prevented the increase in serum and liver triglyceride export when there was a deficiency in estradiol. HPC03 improves OVX-induced fatty liver and lipid metabolism. These findings suggest that HPC03 from postmenopausal women has a protective effect during NAFLD conditions.

Evaluation of Antioxidant Effects of Pumpkin (Cucurbita pepo L.) Seed Extract on Aging- and Menopause-Related Diseases Using Saos-2 Cells and Ovariectomized Rats

“…Although AST is mainly present in the cytoplasm of hepatocytes, AST is found in the cytoplasm and mitochondria of the heart, liver, and kidneys. We accordingly assessed the effects of pumpkin seed extract in the different rat groups by determining the levels of these hepatic markers of liver function, with the results indicating that menopause can influence the levels of AST and ALT, and treatment with pumpkin seed extract can rectify the abnormal levels of these enzymes; the normal range of AST and ALT has been reported in a previous study [43].…”

Protective effect of phytoestrogens on nonalcoholic fatty liver disease in postmenopausal women

“…Furthermore, Trembling et al and Sumida et al confirmed that long-term estrogen deficiency may increase the risk of NAFLD fibrosis in postmenopausal women (Sumida et al, 2020;Trembling et al, 2020). Similarly, the ovariectomized (OVX) rodent models suggest a causal relationship between estrogen deficiency and increased susceptibility to NAFLD (Li et al, 2013;Jeong et al, 2018;Chang et al, 2020). Estrogen deficiency plays an important role in NAFLD pathogenesis in postmenopausal women.…”