Improvement in flow properties and pH stability of compressed, continuous polymer beds for high-performance liquid chromatography

Hjertén, Stellan; Mohammad, Jamil; Nakazato, Kenichi

doi:10.1016/s0021-9673(99)87013-7

Cited by 56 publications

(32 citation statements)

References 10 publications

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“…The mechanism of this chemical radical initiation has been reported by Feng et al [34]. This system has been widely employed to prepare acrylamide-based columns for CGE [35][36][37][38][39], HPLC [40,41], and CEC [42][43][44]. Holdšvendová et al [14] have also described the use of this type of initiation at room temperature for the preparation of methacrylate monolithic columns for capillary LC.…”

Section: Introductionmentioning

confidence: 96%

Peroxodisulfate as a chemical initiator for methacrylate‐ester monolithic columns for capillary electrochromatography

et al. 2008

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Organic monolithic stationary phases for CEC were synthesized in situ in fused-silica capillaries. Polymerization mixtures were composed of butyl methacrylate, ethylene dimethacrylate, and [2-(methacryloyloxy)ethyl]trimethyl ammonium chloride in the presence of a porogenic solvent, using ammonium peroxodisulfate as chemical initiator, and N,N,N',N'-tetramethylethylenediamine to activate the reaction. The influence of the amount of initiator, temperature, and composition of porogenic solvent on the physical and chromatographic properties of monolithic stationary phases has been investigated. A minimum plate height of 14.5 microm was obtained at 18 wt% of 1,4-butanediol in the polymerization mixture. The produced monolithic stationary phases exhibited a good repeatability and batch-to-batch and mixture-to-mixture reproducibility, with RSD values below 5.6% in the electrochromatographic parameters studied. A comparison with columns prepared by thermal initiation with alpha,alpha'-azobisisobutyronitrile (AIBN) was also performed. The most efficient column initiated with peroxodisulfate showed better efficiencies and selectivities than that prepared with AIBN at the same composition mixture.

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Section: Introductionmentioning

confidence: 96%

Peroxodisulfate as a chemical initiator for methacrylate‐ester monolithic columns for capillary electrochromatography

et al. 2008

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“…The pore-size distribution and the porosity of the rod can be easily controlled by selecting the proper porogen and varying the volume ratio of porogen to monomer. Linear decreases in efficiency due to intraparticle w x diffusion at increasing flow-rates are not observed 22,43,[130][131][132][133][134][135][136][137][138] . A number of affinity-membrane cartridges are commercially available.…”

Section: Categories and Characteristics Of Affinity Membrane Cartridgesmentioning

confidence: 91%

Affinity membrane chromatography for the analysis and purification of proteins

Zou

Luo

Zhou

2001

Journal of Biochemical and Biophysical Methods

222

137

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“…Water used throughout the experiments was doubly distilled and purified by a Milli-Q system (Millipore Inc. Milford, MA). 50 mM stock phosphate buffer was prepared by NaH 2 PO 4 and NaOH, and was diluted to the final concentration in the experiments.…”

Section: Methodsmentioning

confidence: 99%

Preparation and characterization of monolithic columns for capillary electrochromatography with weak electroosmotic flow

Zhang

Ping

Zhang

et al. 2003

J of Separation Science

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Preparation and characterization of monolithic columns for capillary electrochromatography with weak electroosmotic flow Monolithic columns of capillary electrochromatography (CEC) with weak electroosmotic flow (EOF) have been prepared by in situ polymerization of butyl methacrylate and ethylene dimethacrylate, without any charged groups in the reaction mixture. The reproducibility of such columns has been proved good no matter whether they are prepared in the same batch or in different batches. In the case of BMA-EDMA monoliths, besides the traditional ternary mixture -1-propanol, 1,4-butanediol, and water, binary porogenic solvents with only alcohols have also been adopted. Compared with ternary porogenic solvents, the design with binary ones allows for fine control of the pore diameter and the formation of the specific surface of the monolithic polymers. The composition of porogenic reagents has also been shown to have an effect on EOF in the column systems. In addition, the Joule heat effect in such columns has been studied by varying the inner diameter of columns. Through the separation of acidic compounds, monolithic columns with low EOF have shown potential in the analysis of charged samples.

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Improvement in flow properties and pH stability of compressed, continuous polymer beds for high-performance liquid chromatography

Cited by 56 publications

References 10 publications

Peroxodisulfate as a chemical initiator for methacrylate‐ester monolithic columns for capillary electrochromatography

Peroxodisulfate as a chemical initiator for methacrylate‐ester monolithic columns for capillary electrochromatography

Affinity membrane chromatography for the analysis and purification of proteins

Preparation and characterization of monolithic columns for capillary electrochromatography with weak electroosmotic flow

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