Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE)

Liu, Li; Chen, Yanyan; Meng, Y.H.; Chen, She; Jin, Gang

doi:10.1016/j.tsf.2010.12.130

Cited by 22 publications

(14 citation statements)

References 17 publications

(21 reference statements)

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“…Its working principle, as explained in detail in the experimental section, is based on the specific interaction of target molecules with ligands on the surface (nonspecific adsorption must be avoided), which results in an increase of the surface density, quantitatively measured by density changes in grayscale values collected on a CCD camera. Thus, the grayscale reflects the dynamics of the biomolecular interactions over the time, similarly to SPR, but with higher sensitivity [43,44,56].…”

Section: Evaluation Of the Immunosensor Performancementioning

confidence: 99%

Nanostructured interfaces with site-specific bioreceptors for immunosensing

Paiva

Almeida

Marquês

et al. 2017

Applied Surface Science

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a b s t r a c tIn this work, we propose a simple and effective approach to build nanostructured immunosensor platforms. The one-step strategy relies on i) the in situ formation of dithiocarbamates from the reaction between carbon disulfide and amine groups, present on protein A, ii) their attachment to gold nanoparticles (AuNPs), and iii) the linkage of the modified AuNPs to the electrode surface, which depends on the strong interaction between gold substrates and sulfur moieties. AuNPs and protein A are used to increase the surface coverage of Immunoglobulin G (IgG) and promote the oriented immobilization of the antibodies on the immunosensing interface. The modified gold surfaces with biomolecules were thoroughly characterized by a combination of techniques: UV-vis spectrophotometry, conventional ellipsometry and atomic force microscopy. The immunosensor performance was assessed in real-time, by surface plasmon resonance and by the highly sensitive total internal reflection imaging ellipsometry, through the specific biorecognition between anti-IgG and the immobilized IgG molecules. We demonstrate that the presence of AuNPs improves the sensitivity of the anti-IgG specific detection, whereas the presence of co-adsorbed CS 2 is responsible for blocking the undesired protein nonspecific adsorption to the gold substrate. Overall, we report a simple and innovative one-step method, to chemically modify gold surfaces with protein A and AuNPs, able to specifically detect antigen/antibody interactions with capability of preventing protein nonspecific adsorption.

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Section: Evaluation Of the Immunosensor Performancementioning

confidence: 99%

Nanostructured interfaces with site-specific bioreceptors for immunosensing

Paiva

Almeida

Marquês

et al. 2017

Applied Surface Science

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“…1e. The TIRIE biosensor is a coupling of the optical system and a microfluidic protein reactor array system by a prism [5,6]. The optical system is an imaging ellipsometer with a light source of Xe lamp and a wavelength 633 nm.…”

Section: Total Internal Reflection Imaging Ellipsometry (Tirie)mentioning

confidence: 99%

“…Microarray biosensor with multiple advantages, such as low sample consumption, fast response time and high throughput, [1][2][3][4] appears to be a promising alternative. A novel microarray biosensor based on total internal reflection imaging ellipsometry (TIRIE) was recently developed for kinetic measurement of biomolecular interaction, which is integrated by imaging ellipsometer and microfluidic reactor system [5,6]. Compared with traditional imaging ellipsometry, TIRIE has higher phase sensitivity since it applies an evanescent wave as optical probe to detect the biomolecular adsorption and interaction process [7].…”

Section: Introductionmentioning

confidence: 99%

Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry

Zhang

Chen

Jin

2011

Sensors and Actuators B: Chemical

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a b s t r a c tWe describe highly sensitive, label-free, real time detection of the biomarker CA19-9 using microarray biosensors based on total internal reflection imaging ellipsometry (TIRIE), in which anti-CA19-9 is immobilized onto the PEGylated phospholipid membrane. Strong resistance against non-specific adsorption of PEGylated lipid membrane allows direct serum assay without the blocking agent. We find that PEG density has an influence on antibody assembly and only a proper amount of PEG is appropriate for antigen binding. Two different methods for antibody assembly are compared and finally protein A is preferred due to its higher protein binding efficiency. The estimated detection limit of CA19-9 is 18.2 U/ml, which is lower than the cut-off value for normal level (37 U/ml). Regression analysis between the results of microarray biosensor and electrochemiluminescence immunoassay (ECLIA) suggests a high correlation (R = 0.989). The capability for real-time monitoring of tumor marker with high sensitivity and selectivity in clinically relevant samples opens up substantial possibilities for early cancer diagnosis and treatment.

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“…Total internal reflection imaging ellipsometry (TIRIE), based on imaging ellipsometry performed in a total internal reflection mode, is an automatic real-time analysis method with high sensitivity and less sample consumption [17]. In TIRIE, an evanescent wave appearing on the gold surface is employed as an optical probe to monitor interactions on the gold surface [17].…”

Section: Introductionmentioning

confidence: 99%

“…In TIRIE, an evanescent wave appearing on the gold surface is employed as an optical probe to monitor interactions on the gold surface [17]. The sensitivity of the TIRIE is proved to be 10 times higher than that of the conventional surface plasma resonance (SPR) in principle [18].…”

Section: Introductionmentioning

confidence: 99%

Optical and Electrochemical Combination Sensor with Poly‐Aniline Film Modified Gold Surface and Its Application for Dissolved Oxygen Detection

Liu

Correia

et al. 2014

Electroanalysis

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Poly‐aniline (PAn) film can be oxidized by contacting oxygen in an electron‐transfer reaction and its redox state, for instance the open circuit potential and the dielectric constant, is quantitatively related to oxygen concentration. This feature may contribute to an improved application of dissolved oxygen (DO) detection with the combined optical and electrochemical sensor. In this paper, PAn is used as a sensing surface to detect DO by the combination sensor composed of electrochemistry and total internal reflection imaging ellipsometry (EC‐TIRIE). Results demonstrate that both optical and electrical signals show a logarithmic correlation with DO concentration (0–20 ppm). Compared with the results obtained only with the gold surface, both the optical and electrical signals of the EC‐TIRIE sensor for DO detection are amplified with the PAn modified gold surface.

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Improvement for sensitivity of biosensor with total internal reflection imaging ellipsometry (TIRIE)

Cited by 22 publications

References 17 publications

Nanostructured interfaces with site-specific bioreceptors for immunosensing

Nanostructured interfaces with site-specific bioreceptors for immunosensing

Serum tumor marker detection on PEGylated lipid membrane using biosensor based on total internal reflection imaging ellipsometry

Optical and Electrochemical Combination Sensor with Poly‐Aniline Film Modified Gold Surface and Its Application for Dissolved Oxygen Detection

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