Improvement and screening of astaxanthin producing mutants of newly isolated Coelastrum sp. using ethyl methane sulfonate induced mutagenesis technique

Tharek, Ameerah; Yahya, Adibah; Salleh, Madihah Md.; Jamaluddin, Haryati; Yoshizaki, Shinji; Hara, Hirofumi; Suzuki, Iwane

doi:10.1016/j.btre.2021.e00673

Cited by 13 publications

(3 citation statements)

References 43 publications

(39 reference statements)

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“…By chemical mutagenesis with EMS, carotenoid content in Coelastrum sp. C1-G1 was increased about 2-fold over its mother strain [39]. The Astaxanthin-overproduction strain H. pluvialis MT 2877 is a mutant by MNNG, which produced 4-fold astaxanthin over the WT strain [111].…”

Section: Genetic Modifications Of Microalgae For Salt Tolerance and C...mentioning

confidence: 98%

Carotenoid Production from Microalgae: Biosynthesis, Salinity Responses and Novel Biotechnologies

et al. 2021

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Microalgae are excellent biological factories for high-value products and contain biofunctional carotenoids. Carotenoids are a group of natural pigments with high value in social production and human health. They have been widely used in food additives, pharmaceutics and cosmetics. Astaxanthin, β-carotene and lutein are currently the three carotenoids with the largest market share. Meanwhile, other less studied pigments, such as fucoxanthin and zeaxanthin, also exist in microalgae and have great biofunctional potentials. Since carotenoid accumulation is related to environments and cultivation of microalgae in seawater is a difficult biotechnological problem, the contributions of salt stress on carotenoid accumulation in microalgae need to be revealed for large-scale production. This review comprehensively summarizes the carotenoid biosynthesis and salinity responses of microalgae. Applications of salt stress to induce carotenoid accumulation, potentials of the Internet of Things in microalgae cultivation and future aspects for seawater cultivation are also discussed. As the global market share of carotenoids is still ascending, large-scale, economical and intelligent biotechnologies for carotenoid production play vital roles in the future microalgal economy.

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Section: Genetic Modifications Of Microalgae For Salt Tolerance and C...mentioning

confidence: 98%

Carotenoid Production from Microalgae: Biosynthesis, Salinity Responses and Novel Biotechnologies

et al. 2021

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“…Glufosinate-resistant mutants of Haematococcus pluvialis and Coelastrum sp. with higher astaxanthin contents than the wildtype have been isolated ( Table 1 and Table S1 ) [ 99 , 110 ].…”

Section: Strategies For Microalgal Strain Improvementmentioning

confidence: 99%

Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production

Trovão

Schüler

Machado³

et al. 2022

Marine Drugs

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Microalgae have become a promising novel and sustainable feedstock for meeting the rising demand for food and feed. However, microalgae-based products are currently hindered by high production costs. One major reason for this is that commonly cultivated wildtype strains do not possess the robustness and productivity required for successful industrial production. Several strain improvement technologies have been developed towards creating more stress tolerant and productive strains. While classical methods of forward genetics have been extensively used to determine gene function of randomly generated mutants, reverse genetics has been explored to generate specific mutations and target phenotypes. Site-directed mutagenesis can be accomplished by employing different gene editing tools, which enable the generation of tailor-made genotypes. Nevertheless, strategies promoting the selection of randomly generated mutants avoid the introduction of foreign genetic material. In this paper, we review different microalgal strain improvement approaches and their applications, with a primary focus on random mutagenesis. Current challenges hampering strain improvement, selection, and commercialization will be discussed. The combination of these approaches with high-throughput technologies, such as fluorescence-activated cell sorting, as tools to select the most promising mutants, will also be discussed.

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“…Kadar astaxanthin pada penelitian ini lebih besar dibandingkan kadar astaxanthin ekstrak aseton Coelastrum sp dengan kadar astaxanthinsebesar 4,51 mg/L [23] , namun lebih sedikit dibandingkan Chlorella zofingiensis dengan kadar astaxanthin sebesar 12,5 mg/L [24] [5] dan Botryococcus brauniidengan kadar astaxanthin sebesar 17,875 mg/L. Selain perbedaan jenis mikroalga yang digunakan untuk ekstraksi, perbedaan nutrisi serta kondisi lingkungan pada media kultur dapat mempengaruhi pembentukan karotenoid dan pigmen astaxanthin [12] [6].…”

Section: Gambar 4 Kurva Standar Astaxanthinunclassified

Penentuan Kadar Astaxanthin, Uji Antiinflamasi Dan Sitotoksik Terhadap Sel Kanker Payudara Ekstrak Aseton Spirulina Platensis

Armaini,

Syafrizayanti,

Zara Aulia

2022

J. Kim. Unand

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Spirulina platensis is a microalgae widely used in the health sector due to its micronutrients, biopigment such as astaxanthin. Astaxanthin is carotenoid belonging to strong antioxidant, which has anti-inflammatory and cytotoxic effects on MCF-7 breast cancer cells. This study aims to determine astaxanthin contents, to investigate the anti-inflammatory activity and cytotoxic potential of breast cancer cells MCF-7 treated with acetone extract from Spirulina platensis. Extraction is done by maceration method. Astaxanthin contents were determined using UV-Vis spectrophotometer set to 479 nm. The anti-inflammatory assays were performed using inhibition of protein denaturation method and cytotoxic assays were performed using the MTT method. From results of study, the extract contained 6,3008 mg/L astaxanthin. The IC50 values for anti-inflammatory and cytotoxic tests were 363,399 mg/L and 66,68 mg/L, respectively. According to the result of the tests, acetone extract from Spirulina platensis showed anti-inflammatory activity and moderate cytotoxic activity against MCF-7 breast cancer cells.

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Improvement and screening of astaxanthin producing mutants of newly isolated Coelastrum sp. using ethyl methane sulfonate induced mutagenesis technique

Cited by 13 publications

References 43 publications

Carotenoid Production from Microalgae: Biosynthesis, Salinity Responses and Novel Biotechnologies

Carotenoid Production from Microalgae: Biosynthesis, Salinity Responses and Novel Biotechnologies

Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production

Penentuan Kadar Astaxanthin, Uji Antiinflamasi Dan Sitotoksik Terhadap Sel Kanker Payudara Ekstrak Aseton Spirulina Platensis

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