Improved wound healing of diabetic foot ulcers using human placenta-derived mesenchymal stem cells in gelatin electrospun nanofibrous scaffolds plus a platelet-rich plasma gel: A randomized clinical trial

Meamar, Rokhsareh; Ghasemi‐Mobarakeh, Laleh; Norouzi, Masoud; Siavash, Mansour; Hamblin, Michael R.; Fesharaki, Mehrafarin

doi:10.1016/j.intimp.2021.108282

Cited by 37 publications

(67 citation statements)

References 46 publications

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“…For example, Fu et al demonstrated the human urine-derived stem cells (hUSCs) could secrete multiple growth factors including vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1), and the hUSCs-seeded electrospun PCL/gelatin nanofiber dressings obviously accelerated wound closure in full-thickness rabbit skin wound models ( Figure 8 E) [ 170 ]. Meamar et al found that the human placenta-derived mesenchymal stem cells (hPDMSCs)-carried electrospun gelatin nanofiber mats containing platelet-rich plasma (PRP) significantly promoted the skin regeneration of human diabetic foot ulcers by effectively reducing inflammation and promoting angiogenesis ( Figure 8 F) [ 171 ]. Lotfi et al developed adipose tissue-derived mesenchymal stem cells (ADMSCs) and keratinocytesco co-seeded electrospun gelatin/chitosan/β-glycerol phosphate nanofiber dressings, which exhibited a significantly enhanced wound closure rate in the rat acute injury models [ 172 ].…”

Section: Electrospun Gelatin-based Nanofiber Mats Loaded With Bioacti...mentioning

confidence: 99%

State-of-the-Art Review of Electrospun Gelatin-Based Nanofiber Dressings for Wound Healing Applications

Sun

2022

Nanomaterials

146

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Electrospun nanofiber materials have been considered as advanced dressing candidates in the perspective of wound healing and skin regeneration, originated from their high porosity and permeability to air and moisture, effective barrier performance of external pathogens, and fantastic extracellular matrix (ECM) fibril mimicking property. Gelatin is one of the most important natural biomaterials for the design and construction of electrospun nanofiber-based dressings, due to its excellent biocompatibility and biodegradability, and great exudate-absorbing capacity. Various crosslinking approaches including physical, chemical, and biological methods have been introduced to improve the mechanical stability of electrospun gelatin-based nanofiber mats. Some innovative electrospinning strategies, including blend electrospinning, emulsion electrospinning, and coaxial electrospinning, have been explored to improve the mechanical, physicochemical, and biological properties of gelatin-based nanofiber mats. Moreover, numerous bioactive components and therapeutic agents have been utilized to impart the electrospun gelatin-based nanofiber dressing materials with multiple functions, such as antimicrobial, anti-inflammation, antioxidation, hemostatic, and vascularization, as well as other healing-promoting capacities. Noticeably, electrospun gelatin-based nanofiber mats integrated with specific functions have been fabricated to treat some hard-healing wound types containing burn and diabetic wounds. This work provides a detailed review of electrospun gelatin-based nanofiber dressing materials without or with therapeutic agents for wound healing and skin regeneration applications.

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Section: Electrospun Gelatin-based Nanofiber Mats Loaded With Bioacti...mentioning

confidence: 99%

State-of-the-Art Review of Electrospun Gelatin-Based Nanofiber Dressings for Wound Healing Applications

Sun

2022

Nanomaterials

146

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“…A recent study by Meamar et al has been conducted using nanofibers containing PDMSCs plus platelet-rich plasma (PRP) for healing DFU. In this study, the 12-week ulcer treatment was divided into three groups; the first group received PDMSCs, the second group received PDMSCs after being coated with PRP gel, and the third group was the control that received the standard wound care [ 122 ]. When comparing the outcome from the three groups, Meamar et al reported that there was a 66% and 71% reduction in wound size in the groups treated with PDMSCs and PDMSCs plus PRP gel, respectively, while only 36% of wound reduction was observed in the control group.…”

Section: Stem Cell Therapymentioning

confidence: 99%

“…A significant improvement was observed in the wound closure and pain-free walking distance in the groups treated with PDMSCs and PDMSCs plus PRP gel compared to the control group. In addition, the biopsy results showed development of new capillaries in both groups treated with PDMSCs and PDMSCs plus PRP gel [ 122 ]. Another recent study by DaVanzo et al investigated the outcomes for DFU patients treated with a cryopreserved placental membrane containing viable cells (vCPM) to other Cellular- and Tissue-Based Products (CTPs).…”

Section: Stem Cell Therapymentioning

confidence: 99%

Stem Cell-Based Therapy: A Promising Treatment for Diabetic Foot Ulcer

et al. 2022

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Diabetic foot ulcer (DFU) is a severe complication of diabetes and a challenging medical condition. Conventional treatments for DFU have not been effective enough to reduce the amputation rates, which urges the need for additional treatment. Stem cell-based therapy for DFU has been investigated over the past years. Its therapeutic effect is through promoting angiogenesis, secreting paracrine factors, stimulating vascular differentiation, suppressing inflammation, improving collagen deposition, and immunomodulation. It is controversial which type and origin of stem cells, and which administration route would be the most optimal for therapy. We reviewed the different types and origins of stem cells and routes of administration used for the treatment of DFU in clinical and preclinical studies. Diabetes leads to the impairment of the stem cells in the diseased patients, which makes it less ideal to use autologous stem cells, and requires looking for a matching donor. Moreover, angioplasty could be complementary to stem cell therapy, and scaffolds have a positive impact on the healing process of DFU by stem cell-based therapy. In short, stem cell-based therapy is promising in the field of regenerative medicine, but more studies are still needed to determine the ideal type of stem cells required in therapy, their safety, proper dosing, and optimal administration route.

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“…This scaffold then implanted to the patients with diabetic foot ulcers (DFUs) and then various clinical parameters were assessed after 12-week. Indeed, wound healing, new capillary formation, and pain-free walking distance were significantly increased by the implantation of placenta-derived MSCs seeded GNS ( Meamar et al, 2021 ).…”

Section: Clinical Trials Of Stem Cell-based Bioengineering Therapeuticsmentioning

confidence: 99%

Enhancing Stem Cell-Based Therapeutic Potential by Combining Various Bioengineering Technologies

Hong

2022

Front. Cell Dev. Biol.

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Stem cell-based therapeutics have gained tremendous attention in recent years due to their wide range of applications in various degenerative diseases, injuries, and other health-related conditions. Therapeutically effective bone marrow stem cells, cord blood- or adipose tissue-derived mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), and more recently, induced pluripotent stem cells (iPSCs) have been widely reported in many preclinical and clinical studies with some promising results. However, these stem cell-only transplantation strategies are hindered by the harsh microenvironment, limited cell viability, and poor retention of transplanted cells at the sites of injury. In fact, a number of studies have reported that less than 5% of the transplanted cells are retained at the site of injury on the first day after transplantation, suggesting extremely low (<1%) viability of transplanted cells. In this context, 3D porous or fibrous national polymers (collagen, fibrin, hyaluronic acid, and chitosan)-based scaffold with appropriate mechanical features and biocompatibility can be used to overcome various limitations of stem cell-only transplantation by supporting their adhesion, survival, proliferation, and differentiation as well as providing elegant 3-dimensional (3D) tissue microenvironment. Therefore, stem cell-based tissue engineering using natural or synthetic biomimetics provides novel clinical and therapeutic opportunities for a number of degenerative diseases or tissue injury. Here, we summarized recent studies involving various types of stem cell-based tissue-engineering strategies for different degenerative diseases. We also reviewed recent studies for preclinical and clinical use of stem cell-based scaffolds and various optimization strategies.

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Improved wound healing of diabetic foot ulcers using human placenta-derived mesenchymal stem cells in gelatin electrospun nanofibrous scaffolds plus a platelet-rich plasma gel: A randomized clinical trial

Cited by 37 publications

References 46 publications

State-of-the-Art Review of Electrospun Gelatin-Based Nanofiber Dressings for Wound Healing Applications

State-of-the-Art Review of Electrospun Gelatin-Based Nanofiber Dressings for Wound Healing Applications

Stem Cell-Based Therapy: A Promising Treatment for Diabetic Foot Ulcer

Enhancing Stem Cell-Based Therapeutic Potential by Combining Various Bioengineering Technologies

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