Improved water use efficiency and shorter life cycle of Nicotiana tabacum due to modification of guard and vascular companion cells

Müller, Gabriela; Lara, Marı́a V.; Oitaven, Pablo; Andreo, Carlos S.; Maurino, Verónica G.; Drincovich, Marı́a F.

doi:10.1038/s41598-018-22431-5

Cited by 19 publications

(18 citation statements)

References 65 publications

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“…Thus our tower footprint is approximated using the height of the tower, wind speed, wind direction, friction velocity, Monin Ohbukov stability parameter, and the standard deviation of the cross-stream wind component. Similar to others 9 , 10 , we identified polygonal tundra landforms using high-resolution 0.6 m pan-sharpened 2008 Quickbird 2 multispectral imagery and digitized polygonal landforms in a 300-m radius of the tower. The proportion of landforms within 80% of the tower footprint were used to approximate NEE using DOS-TEM simulated fluxes and compare with eddy covariance-derived NEE observations (Fig.…”

Section: Methodsmentioning

confidence: 82%

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Local-scale Arctic tundra heterogeneity affects regional-scale carbon dynamics

et al. 2020

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In northern Alaska nearly 65% of the terrestrial surface is composed of polygonal ground, where geomorphic tundra landforms disproportionately influence carbon and nutrient cycling over fine spatial scales. Process-based biogeochemical models used for local to Pan-Arctic projections of ecological responses to climate change typically operate at coarse-scales (1km2–0.5°) at which fine-scale (<1km2) tundra heterogeneity is often aggregated to the dominant land cover unit. Here, we evaluate the importance of tundra heterogeneity for representing soil carbon dynamics at fine to coarse spatial scales. We leveraged the legacy of data collected near Utqiaġvik, Alaska between 1973 and 2016 for model initiation, parameterization, and validation. Simulation uncertainty increased with a reduced representation of tundra heterogeneity and coarsening of spatial scale. Hierarchical cluster analysis of an ensemble of 21st-century simulations reveals that a minimum of two tundra landforms (dry and wet) and a maximum of 4km2 spatial scale is necessary for minimizing uncertainties (<10%) in regional to Pan-Arctic modeling applications.

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Section: Methodsmentioning

confidence: 82%

“… The polygonal tundra map 10 , 13 is projected over ArcGIS World Imagery basemap (Sources: Esri, DigitalGlobe, GeoEye, i-cubed, USDA FSA, USGS, AEX, Getmapping, Aerogrid, IGN, IGP, swisstopo, and the GIS User Community). …”

Section: Introductionmentioning

confidence: 99%

Local-scale Arctic tundra heterogeneity affects regional-scale carbon dynamics

et al. 2020

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“…These correlations results clearly indicate that an increase in stomatal density, impacts in increased g max and increased Δ 13 C values in rice. and gaseous exchange in rice plants, it can be a potential target for modification in order to increase and sustain rice yield with minimal water input (Müller et al 2018;Franks et al 2015;Flexas 2016;Zhang et al 2012). Being a positive regulator of photosynthesis (Kusumi et al 2012), manipulation of stomatal traits, especially the number and size, could pave new ways to break the rice yield barrier especially in areas with limited water availability (Oshumi et al 2007;Xu et al 2009).…”

Section: Structure-function Relationships In Rice Stomatal Traitsmentioning

confidence: 99%

Natural Diversity in Stomatal Features of Cultivated and Wild Oryza Species

Chatterjee

Thakur

Nepomuceno

et al. 2020

Rice

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Background: Stomata in rice control a number of physiological processes by regulating gas and water exchange between the atmosphere and plant tissues. The impact of the structural diversity of these micropores on its conductance level is an important area to explore before introducing stomatal traits into any breeding program in order to increase photosynthesis and crop yield. Therefore, an intensive measurement of structural components of stomatal complex (SC) of twenty three Oryza species spanning the primary, secondary and tertiary gene pools of rice has been conducted. Results: Extensive diversity was found in stomatal number and size in different Oryza species and Oryza complexes. Interestingly, the dynamics of stomatal traits in Oryza family varies differently within different Oryza genetic complexes. Example, the Sativa complex exhibits the greatest diversity in stomatal number, while the Officinalis complex is more diverse for its stomatal size. Combining the structural information with the Oryza phylogeny revealed that speciation has tended towards increasing stomatal density rather than stomatal size in rice family. Thus, the most recent species (i.e. the domesticated rice) eventually has developed smaller yet numerous stomata. Along with this, speciation has also resulted in a steady increase in stomatal conductance (anatomical, g max) in different Oryza species. These two results unambiguously prove that increasing stomatal number (which results in stomatal size reduction) has increased the stomatal conductance in rice. Correlations of structural traits with the anatomical conductance, leaf carbon isotope discrimination (Δ 13 C) and major leaf morphological and anatomical traits provide strong supports to untangle the ever mysterious dependencies of these traits in rice. The result displayed an expected negative correlation in the number and size of stomata; and positive correlations among the stomatal length, width and area with guard cell length, width on both abaxial and adaxial leaf surfaces. In addition, g max is found to be positively correlated with stomatal number and guard cell length. The Δ 13 C values of rice species showed a positive correlation with stomatal number, which suggest an increased water loss with increased stomatal number. Interestingly, in contrast, the Δ 13 C consistently shows a negative relationship with stomatal and guard cell size, which suggests that the water loss is less when the stomata are larger. Therefore, we hypothesize that increasing stomatal size, instead of numbers, is a better approach for breeding programs in order to minimize the water loss through stomata in rice.

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“…Effects similar to those of GCHXK were obtained in tobacco ( Nicotiana tabacum ) plants expressing nicotinamide adenine dinucleotide phosphate (NADP)-malic enzyme (NADP-ME) in their guard cells and vascular companion cells (Müller et al, 2018). Expression of NADP-ME reduced malate content and decreased stomatal aperture, but nevertheless increased the net CO 2 fixation rate and phloem sugar translocation.…”

Section: Discussionmentioning

confidence: 91%

Guard-Cell Hexokinase Increases Water-Use Efficiency Under Normal and Drought Conditions

et al. 2019

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Water is a limiting resource for many land plants. Most of the water taken up by plants is lost to the atmosphere through the stomata, which are adjustable pores on the leaf surface that allow for gas exchange between the plant and the atmosphere. Modulating stomatal activity might be an effective way to reduce plants’ water consumption and enhance their productivity under normal, as well as water-limiting conditions. Our recent discovery of stomatal regulation by sugars that is mediated by guard-cell hexokinase (HXK), a sugar-sensing enzyme, has raised the possibility that HXK might be used to increase plant water-use efficiency (WUE; i.e., carbon gain per unit of water). We show here that transgenic tomato and Arabidopsis plants with increased expression of HXK in their guard cells (GCHXK plants) exhibit reduced transpiration and higher WUE without any negative effects on growth under normal conditions, as well as drought avoidance and improved photosynthesis and growth under limited-water conditions. Our results demonstrate that exclusive expression of HXK in guard cells is an effective tool for improving WUE, and plant performance under drought.

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Improved water use efficiency and shorter life cycle of Nicotiana tabacum due to modification of guard and vascular companion cells

Cited by 19 publications

References 65 publications

Local-scale Arctic tundra heterogeneity affects regional-scale carbon dynamics

Local-scale Arctic tundra heterogeneity affects regional-scale carbon dynamics

Natural Diversity in Stomatal Features of Cultivated and Wild Oryza Species

Guard-Cell Hexokinase Increases Water-Use Efficiency Under Normal and Drought Conditions

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