Improved visualization of dopaminergic neurons in nematodes using the glyoxylic acid fluorescence method

Sharpe, M. J.; Atkinson, H. J.

doi:10.1111/j.1469-7998.1980.tb07772.x

Cited by 28 publications

(7 citation statements)

References 24 publications

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“…Catecholamine-containing cells were visualized by the glyoxilic acid-induced fluorescence technique (de la Torre and Surgeon. 1976;Sharp and Atkinson, 1980). Larvae were incubated in a solution containing 2% glyoxilic acid (Fluka) and 4% sucrose in 0.1 M phosphate buffer (pH 7.4, 1 8C) for 10-20 min.…”

Section: Methodsmentioning

confidence: 99%

Nervous System of the Tornaria Larva (Hemichordata: Enteropneusta). A Histochemical and Ultrastructural Study

Dautov

Nezlin

1992

The Biological Bulletin

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Transmission electron microscopy (TEM) and histochemical approaches were used to investigate the topology and ultrastructure of the nervous system of the tornaria larva of an enteropneust, Balanoglossus proterogonius. Cholinesterase activity was detected in the epithelium of the pre- and postoral ciliary bands. Groups of catecholamine-containing cells (CA) were detected at the anterior tip of larva, in the ventral epidermis behind the mouth, and in the stomach wall near its junction with the intestine. Single CA neurons were detected in the telotroch epithelium. Axon tracts are described in ciliary band epithelia. At the base of the aboral plate, epithelial nerve cells form a ganglion-like cluster. Single neuron-like cells and single axons and axonal tracts were found in the epithelium of digestive tract. The data were compared with ones from the literature and with those obtained from other marine invertebrate larvae. The properties of the neural elements and their possible functions are discussed.

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Section: Methodsmentioning

confidence: 99%

Nervous System of the Tornaria Larva (Hemichordata: Enteropneusta). A Histochemical and Ultrastructural Study

Dautov

Nezlin

1992

The Biological Bulletin

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“…Using a glyoxylic acid fluorescence method, dopaminelike fluorescence has been identified in individual neurons of J2 G. rostochiensis (Sharpe and Atkinson 1980). The presence of dopamine has also been investigated in M. incognita using both HPLC and immunohistochemistry (Stewart et al 2001).…”

Section: Biogenic Aminesmentioning

confidence: 99%

Neurobiology of plant parasitic nematodes

Holden‐Dye

Walker

2011

Invert Neurosci

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The regulatory constraints imposed on use of chemical control agents in agriculture are rendering crops increasingly vulnerable to plant parasitic nematodes. Thus, it is important that new control strategies which meet requirements for low toxicity to non-target species, vertebrates and the environment are pursued. This would be greatly facilitated by an improved understanding of the physiology and pharmacology of these nematodes, but to date, these microscopic species of the Phylum Nematoda have attracted little attention in this regard. In this review, the current information available for neurotransmitters and neuromodulator in the plant parasitic nematodes is discussed in the context of the more extensive literature for other species in the phylum, most notably Caenorhabditis elegans and Ascaris suum. Areas of commonality and distinctiveness in terms of neurotransmitter profile and function between these species are highlighted with a view to improving understanding of to what extent, and with what level of confidence, this information may be extrapolated to the plant parasitic nematodes.

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“…The neurotransmitters dopamine, serotonin, and acetylcholine have been detected in several nematode species (Sharp and Akinson 1980;Stewart et al 2001) where they play a role in several aspects of nematode behavior. In Caenorhabditis elegans, the free-living nematode, exposure to exogenous dopamine causes numerous negative effects including paralysis, as well as inhibition of egg laying and defecation (Schafer and Kenyon 1995;Weinshenker et al 1995).…”

Section: Resultsmentioning

confidence: 99%

Chemotaxis Disruption in Pratylenchus Scribneri by Tall Fescue Root Extracts and Alkaloids

et al. 2009

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Tall fescue (Festuca arundinacea) forms a symbiotic relationship with the clavicipitalean fungal endophyte Neotyphodium coenophialum. Endophyte-infected grass is tolerant to nematode, but the factors responsible are unknown. One objective of this work was to determine if root extracts of tall fescue effected chemoreceptor activity of Pratylenchus scribneri by using an in vitro chemoreception bioassay. Another objective was to determine if specific ergot alkaloids (ergovaline, ergotamine, a-ergocryptine, ergonovine), and loline alkaloids, all produced by the fungal endophyte, altered chemotaxis with this bioassay. Methanolic extract from roots altered chemotaxis activities in this nematode but only from roots of plants cultured 45 > or = d, which repelled nematodes. Extracts prepared from noninfected grasses were attractants. This assay indicated that the alkaloids were either repellents or attractants. N-formylloline was an attractant at concentrations of 20 microg/ml and lower, while at higher concentrations it was a repellent. Ergovaline, the major ergot alkaloid produced by the endophyte, was repellent at both high and low concentrations and caused complete death of the nematodes.

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Improved visualization of dopaminergic neurons in nematodes using the glyoxylic acid fluorescence method

Cited by 28 publications

References 24 publications

Nervous System of the Tornaria Larva (Hemichordata: Enteropneusta). A Histochemical and Ultrastructural Study

Nervous System of the Tornaria Larva (Hemichordata: Enteropneusta). A Histochemical and Ultrastructural Study

Neurobiology of plant parasitic nematodes

Chemotaxis Disruption in Pratylenchus Scribneri by Tall Fescue Root Extracts and Alkaloids

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