Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (&lt;em&gt;Lactuca sativa&lt;/em&gt; L.) and Crop Wild Relatives (&lt;em&gt;Lactuca&lt;/em&gt; spp.)

Medina-Lozano, Inés; Bertolín, Juan Ramón; Zufiaurre, Raquel; Díaz, Aurora

doi:10.3791/61440

Cited by 5 publications

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“…The total content of vitamin C [the sum of ascorbic acid (AA) and dehydroascorbic acid (DHAA)] was determined by the 2,6-dichloroindophenol (DCIP) spectrophotometric method ( 20 ) and the modified procedure for extraction applied by Medina et al ( 21 ). To 0.25 g of the freeze-dried sample, 2.5 ml of the extractant reactive (3% (m/v) metaphosphoric acid and 8% (v/v) acetic acid) was added and homogenized in a vortex for 1 min.…”

Section: Methodsmentioning

confidence: 99%

Bioactive compounds, antioxidant activity, and mineral content of bróquil: A traditional crop of Brassica oleracea var. italica

et al. 2023

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Brassicaceae edible plants are rich in bioactive compounds and promote health benefits. However, there is less interest in expanding knowledge about the Brassica cultivars to date. In particular, underutilized species and local cultivars could constitute a source of agrodiversity in adapting to the territory with likely higher contents of nutraceutical compounds. In this context, Bróquil (Brassica oleracea var. italica) is a traditional Brassicaceae crop grown in the Spanish region of Aragón. Currently, it is cultivated mainly in family orchards for autoconsumption and, in minority, in small farms for local markets. This study evaluates a collection of 13 bróquil landraces from the Spanish Vegetable Genebank of the Agrifood Research and Technology Center of Zaragoza (BGHZ-CITA), describing their mineral contents, bioactive compounds, and antioxidant activities, including a broccoli commercial variety “Parthenon” as the control. The study reports data on the health-promoting nutrients and antioxidants of bróquil for the first time. Under our experimental conditions, we found that bróquil has a great variability for these compounds that showed on average similar or higher levels than the broccoli control. The different bróquil landraces also revealed variability in both intraccessions and interaccessions due to the lack of a formal breeding selection. Despite this variability, we highlight accession HB5 that corresponds to Headed Bróquil BGHZ6685. In particular, we can stand out its antioxidant activity of 87.07 ± 0.81%I, total phenolic content of 13.21 ± 0.53 mg GAE g−1 dw, total flavonoid content of 14.50 ± 1.29 mg QE g−1 dw, total glucosinolate content of 43.70 ± 1.09 mg SnE g−1 dw, and vitamin C content of 7.21 ± 0.13 mg AA g−1 dw. Regarding bróquil mineral composition, K was the highest macroelement (22.66–33.62 mg g−1 dw), followed by Ca, P, and S whose values were relatively lower compared to K. Mg and Na showed the lowest values. Among the microelements evaluated (Mn, Zn, and Fe), iron was the most abundant detected, higher in all bróquil accessions than in broccoli, except for one accession. Therefore, the results reported for bróquil landraces show promising nutritional quality. This could lead to an increase in agrobiodiversity and contribute to a more diversified and healthy diet.

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Section: Methodsmentioning

confidence: 99%

Bioactive compounds, antioxidant activity, and mineral content of bróquil: A traditional crop of Brassica oleracea var. italica

et al. 2023

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“…Two leaves representing the whole plant (i.e., inner and outer leaves in plants that grow forming rosettes or heads), as well as parts of the stem in the case of L. dregeana (it grows as a bush), were harvested ( Figure 1 ). All the plant material was immediately frozen using liquid nitrogen and then preserved at −80°C until their lyophilization, as described previously ( Medina-Lozano et al., 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…Both types of compounds have health-promoting properties mainly due to their antioxidant activity ( Cásedas et al., 2020 ; Xu et al., 2022 ) and have been reported to participate in the plant defense against biotic stresses and in mechanisms of tolerance to abiotic stresses ( Gould, 2004 ; Locato et al., 2013 and references herein). Vitamin C or total ascorbic acid (TAA) is made up of ascorbic acid (AA) and its oxidation product, dehydroascorbic acid (DHAA) ( Medina-Lozano et al., 2020 ). Vitamin C is an indicator of the quality of many fruits and vegetables that must be incorporated in the diet as it is an essential micronutrient for humans ( Carr and Frei, 1999 ).…”

Section: Introductionmentioning

confidence: 99%

Impact of drought stress on vitamin C and anthocyanin content in cultivated lettuces (Lactuca sativa L.) and wild relatives (Lactuca spp.)

Medina-Lozano,

Bertolín,

Díaz

2024

Front. Plant Sci.

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IntroductionLettuce production and quality could be seriously affected by the increasingly limited water resources.MethodsThe effect of drought on the content of two antioxidant compounds, vitamin C and anthocyanins, in five cultivated lettuces and two wild relatives was assessed for 2 years.Results and discusionIn leaf samples, Lactuca wild species generally had a higher content of total vitamin C than the cultivated lettuces. In contrast, the commercial varieties usually contained more total anthocyanins than the wild species. Total vitamin C decreased with the drought stress in all accessions, commercial varieties, and lettuce wild relatives, with this tendency being consistent and reproducible across the 2 years. These differences were significant in the case of the green commercial varieties ‘Winter Crop’ (in 2020/2021) and ’Dolomiti G12’ (in 2021/2022) and very significant in the red commercial variety ’Red Sails’ (in 2020/2021). However, the only group in which the effect of drought was either significant or very significant in both years was the wild species, Lactuca homblei and Lactuca dregeana, and in the latter also in both tissues (leaf and stem) analyzed. Water stress resulted in an increase of the total anthocyanin content in the leaves from all the accessions, both red commercial varieties and wild relatives, in both years. The most significant enrichment and the only one being either significant or very significant in both years was observed in one of the wild relatives assayed (L. homblei). Stems (L. dregeana) contained more anthocyanins than leaves under control conditions, and it was exactly the opposite under drought. Changes in anthocyanins in the two tissues in response to drought stress were in opposite directions, increasing in leaves and decreasing in stems. This could suggest a translocation of anthocyanins as a first quick mechanism to cope with a severe lack of water. In conclusion, anthocyanins (unlike vitamin C) could play a role in the mechanisms deployed by the plant to tolerate drought stress. The wild species with a robust significant enrichment in anthocyanins as a response to drought (L. homblei) is a promising plant material to breed more resilient lettuces.

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“…In the first case, it is possible to process a high number of samples in a short period of time, though the identification capacity is limited. The hyphenated methods, on the other hand, are undoubtedly more powerful when it comes to identifying and quantifying metabolites, and there is also the possibility of reducing the running times by using more advanced techniques in chromatography (i.e., ultra-high-performance liquid chromatography (UPLC) instead of high-performance liquid chromatography (HPLC) [ 129 ]). In any case, the metabolite identification generally requires the availability of libraries in order to compare the spectra obtained.…”

Section: Exploring Biodiversity: Searching For Outstanding Materialsmentioning

confidence: 99%

Applications of Genomic Tools in Plant Breeding: Crop Biofortification

Medina-Lozano

Díaz

2022

IJMS

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Crop breeding has mainly been focused on increasing productivity, either directly or by decreasing the losses caused by biotic and abiotic stresses (that is, incorporating resistance to diseases and enhancing tolerance to adverse conditions, respectively). Quite the opposite, little attention has been paid to improve the nutritional value of crops. It has not been until recently that crop biofortification has become an objective within breeding programs, through either conventional methods or genetic engineering. There are many steps along this long path, from the initial evaluation of germplasm for the content of nutrients and health-promoting compounds to the development of biofortified varieties, with the available and future genomic tools assisting scientists and breeders in reaching their objectives as well as speeding up the process. This review offers a compendium of the genomic technologies used to explore and create biodiversity, to associate the traits of interest to the genome, and to transfer the genomic regions responsible for the desirable characteristics into potential new varieties. Finally, a glimpse of future perspectives and challenges in this emerging area is offered by taking the present scenario and the slow progress of the regulatory framework as the starting point.

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Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (<em>Lactuca sativa</em> L.) and Crop Wild Relatives (<em>Lactuca</em> spp.)

Cited by 5 publications

References 10 publications

Bioactive compounds, antioxidant activity, and mineral content of bróquil: A traditional crop of Brassica oleracea var. italica

Bioactive compounds, antioxidant activity, and mineral content of bróquil: A traditional crop of Brassica oleracea var. italica

Impact of drought stress on vitamin C and anthocyanin content in cultivated lettuces (Lactuca sativa L.) and wild relatives (Lactuca spp.)

Applications of Genomic Tools in Plant Breeding: Crop Biofortification

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