Improved Techniques in Paper Chromatography of Carbohydrates

Jeanes, Allene; Wise, C. S.; Dimler, R. J.

doi:10.1021/ac60051a007

Cited by 435 publications

(98 citation statements)

References 23 publications

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“…&Amylase Table 1 Table 2 (Sigma) was assayed at pH 5 and a-amylase (saliva source) treatment was carried out in the presence of 0.01 M NaCl. Hydrolysis products were identified by paper chromatography with butanol-pyridine-water (6:4:3) as solvent [5] and paper electrophoresis in pyridine acetate buffer, pH 6.4, for 2 hr at 1000 V. All other materials were as described elsewhere [6].…”

Section: Enzyme Assaymentioning

confidence: 99%

Particulate UDP‐glucose: Protein transglucosylase from potato tuber

Lavintman¹,

Cardini²

1973

FEBS Letters

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Section: Enzyme Assaymentioning

confidence: 99%

Particulate UDP‐glucose: Protein transglucosylase from potato tuber

Lavintman¹,

Cardini²

1973

FEBS Letters

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“…The product was characterized as maltose by paper chromatography in butanol-pyridinewater (6:4:3, by vol.) [11]. The reducing power was measured by the Somogyi-Nelson 112, 131 method.…”

Section: Analyticalmentioning

confidence: 99%

Action Patterns of Phosphorylase and Glycogen Synthetase on Glycogen

Parodi¹,

Mordoh²,

Krisman³

et al. 1970

European Journal of Biochemistry

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The action patterns of liver and muscle glycogen synthetases and of muscle phosphorylase b on glycogen samples of different molecular weight and on ,&amylase limit dextrins were studied. For this purpose a method for measuring the number of newly added glucose residues that are a t non-reducing ends was developed.It was found that glucose transfer to the non-reducing ends of glycogen catalyzed by liver glycogen synthetase and muscle phosphorylase followed a Poisson distribution.The number of outer chains in the glycogen molecules available to both enzymes appeared to be smaller than the actual number of outer chains in the polysaccharide. The number of such available chains diminished as the glycogen was heavier. For the same glycogen sample, the number of available chains to phosphorylase appeared to be equal or smaller than that to glycogen synthetase.

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“…(Hanes & Isherwood, 1949); butan-I-ol-pyridine-water (6:4:3, by vol.) (Jeanes, Wise & Dimler, 1951) was used for the separation of glycosides and hexoses. Material was detected on paper chromatograms by the periodate-Schiff (Hanes & Ishwerood, 1949).…”

Section: E T H O D Smentioning

confidence: 99%