Improved survival of cardiac cells on surface modified electrospun nanofibers

Pushp, Pallavi; Ferreira, Frederico Castelo; Gupta, Mukesh Kumar

doi:10.1134/s0965545x17040058

Cited by 8 publications

(10 citation statements)

References 39 publications

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“…The primary neonatal CMs were seeded on PVA-PVP patches by static method after dip-coating with collagen, essentially as we described earlier . The cell attachment assay was performed as describe earlier (Pushp & Ferreira, Cabral, et al, 2017).…”

Section: In Vitro Characterization Of Pva-pvp Patches With Primary Neonatal Cmsmentioning

confidence: 99%

Plasticized poly(vinylalcohol) and poly(vinylpyrrolidone) based patches with tunable mechanical properties for cardiac tissue engineering applications

Pushp

Bhaskar

Kelkar

et al. 2021

Biotech & Bioengineering

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Polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP) are the two most investigated biopolymers for various tissue engineering applications. However, their poor tensile strength renders them unsuitable for cardiac tissue engineering (CTE).In this study, we developed and evaluated PVA-PVP-based patches, plasticized with glycerol or propylene glycol (0.1%-0.4%; v:v), for their application in CTE. The cardiac patches were evaluated for their physico-chemical (weight, thickness, folding endurance, FT-IR, and swelling behavior) and mechanical properties. The optimized patches were characterized for their ability to support in vitro attachment, viability, proliferation, and beating behavior of neonatal mouse cardiomyocytes (CMs). In vivo evaluation of the cardiac patches was done under the subcutaneous skin pouch and heart of rat models. Results showed that the optimized molar ratio of PVA:PVP with plasticizers (0.3%; v-v) resulted in cardiac patches, which were dry at room temperature and had desirable folding endurance of at least 300, a tensile strength of 6-23 MPa and, percentage elongation at break of more than 250%.Upon contact with phosphate-buffered saline, these PVA-PVP patches formed hydrogel patches having the tensile strength of 1.3-3.0 MPa. The patches supported the attachment, viability, and proliferation of primary neonatal mouse CMs and were nonirritant and noncorrosive to cardiac cells. In vivo transplantation of cardiac patches into a subcutaneous pouch and on the heart of rat models revealed them to be biodegradable, biocompatible, and safe for use in CTE applications.

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Section: In Vitro Characterization Of Pva-pvp Patches With Primary Neonatal Cmsmentioning

confidence: 99%

Plasticized poly(vinylalcohol) and poly(vinylpyrrolidone) based patches with tunable mechanical properties for cardiac tissue engineering applications

Pushp

Bhaskar

Kelkar

et al. 2021

Biotech & Bioengineering

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“…Based on the results of previous study (Pushp et al, 2017), UCMderived MSCs cultured on random and aligned PCL nanofibers, iPSCs were cultured on aligned PCL nanofibers, and were compared with those cultured on TCP plates, which are conventionally considered "gold standard" for comparison. Both Matrigel™ and Synthemax™ coating was evaluated in promoting attachment, proliferation, and differentiation of iPSCs on PCL nanofibrous matrices.…”

Section: In Vitro Culture and Differentiation Of Ipscs Into Cms On mentioning

confidence: 99%

“…A 6% (wt:vol) solution of PCL was prepared in hexafluoro-2-propanol (HFP) and loaded into a 10 ml plastic syringe fitted with hypodermic needle (18 G) for fabrication of aligned PCL nanofiber mat as described previously (Pushp, Ferreira, Cabral, & Gupta, 2017). A random and aligned PCL nanofiber was electrospun with optimized parameters using electrospinning machine.…”

Section: Fabrication Of Nanofibers By Electrospinningmentioning

confidence: 99%

Functional comparison of beating cardiomyocytes differentiated from umbilical cord‐derived mesenchymal/stromal stem cells and human foreskin‐derived induced pluripotent stem cells

Pushp

Sahoo

Ferreira

et al. 2019

J Biomedical Materials Res

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In recent years, stem cell-based therapies shown to have promising effects on the clinical management of ischemic heart disease. Moreover, stem cells differentiation into cardiomyocytes (CMs) can overcome the cell source limitations. The current research involves the isolation and expansion of mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPSCs), their differentiation into CMs and subsequent construction of tissue-engineered myocardium supported by random and aligned polycaprolactone (PCL) nanofibrous matrices (av. dia: 350-850 nm). Umbilical cord matrix (UCM)-derived MSCs were isolated successfully by routine enzymatic digestion and a nonenzymatic explant culture method and characterized by their morphology, differentiation into different lineages, and surface marker expression. Treatment of UCM-derived MSCs with 5-azacytidine (5 μM) induced their differentiation into putative cardiac cells, as revealed by the expression of cardiac-specific troponin T (cTnT), smooth muscles actin, myogenin (MYOG), smoothelin, cardiac α-actin genes and cTnT, α-actinin proteins by RT-PCR and immunocytochemistry, respectively. However, no beating cells were observed in differentiated MSCs. On the other hand, adult human foreskin-derived iPSCs cultured on Matrigel™-coated aligned PCL nanofibrous matrices showed anisotropic behavior along the PCL nanofibers and, upon differentiation, expressed cardiac-specific cTnT (23.34 vs. 32.55%) proteins and showed more synchronized beating than those differentiated on Matrigel™-coated tissue culture coated polystyrene surfaces. Moreover, aligned PCL nanofibers are able to promote cells orientation parallel to the fibers, thus providing an effective way to control anisotropic nature under in vitro condition.

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“…Collagen is the most abundant protein of ECM. The glycosaminoglycans which connects the collagen matrix acts both on structural and non-structural factors for integrity and stability [25,26]. The influence of orientation of polyurethane (PU) and hybrid PU/Collagen random and aligned nanofibers on cell proliferation and adhesion was demonstrated in smooth muscle cells [27].…”

Section: Introductionmentioning

confidence: 99%

ECM Mimetic Electrospun Porous Poly (L-lactic acid) (PLLA) Scaffolds as Potential Substrates for Cardiac Tissue Engineering

et al. 2020

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Cardiac tissue engineering (CTE) aims to generate potential scaffolds to mimic extracellular matrix (ECM) for recreating the injured myocardium. Highly porous scaffolds with properties that aid cell adhesion, migration and proliferation are critical in CTE. In this study, electrospun porous poly (l-lactic acid) (PLLA) porous scaffolds were fabricated and modified with different ECM derived proteins such as collagen, gelatin, fibronectin and poly-L-lysine. Subsequently, adult human cardiac fibroblasts (AHCF) were cultured on the protein modified and unmodified fibers to study the cell behavior and guidance. Further, the cytotoxicity and reactive oxygen species (ROS) assessments of the respective fibers were performed to determine their biocompatibility. Excellent cell adhesion and proliferation of the cardiac fibroblasts was observed on the PLLA porous fibers regardless of the surface modifications. The metabolic rate of cells was on par with the conventional cell culture ware while the proliferation rate surpassed the latter by nearly two-folds. Proteome profiling revealed that apart from being an anchorage platform for cells, the surface topography has modulated significant expression of the cellular proteome with many crucial proteins responsible for cardiac fibroblast growth and proliferation.

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Improved survival of cardiac cells on surface modified electrospun nanofibers

Cited by 8 publications

References 39 publications

Plasticized poly(vinylalcohol) and poly(vinylpyrrolidone) based patches with tunable mechanical properties for cardiac tissue engineering applications

Plasticized poly(vinylalcohol) and poly(vinylpyrrolidone) based patches with tunable mechanical properties for cardiac tissue engineering applications

Functional comparison of beating cardiomyocytes differentiated from umbilical cord‐derived mesenchymal/stromal stem cells and human foreskin‐derived induced pluripotent stem cells

ECM Mimetic Electrospun Porous Poly (L-lactic acid) (PLLA) Scaffolds as Potential Substrates for Cardiac Tissue Engineering

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