Improved separation of amino acids with a new solvent system for two-dimensional paper chromatography

Ambe, K. S.; Tappel, Al L.

doi:10.1016/s0021-9673(01)92900-0

Cited by 22 publications

(4 citation statements)

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“…The concentrated extracts plus known standards were chromatographed and the papers radioautographed. In addition to the solvents described, solvents containing sec-butanol, tertbutanol, butanone, diethylamine, and water (Ambe and Tappel, 1961), and n-butanol, glacial acetic acid, and water (iMizell and Simpson, C1402 fixation in the presence of ribose. The numbers on film indicate the sequence of flow of solvents.…”

Section: Resultsmentioning

confidence: 99%

Characteristics and Intermediates of Short-Term C ¹⁴ O ₂ Incorporation During Ribose Oxidation by Hydrogenomonas facilis

McFadden

Homann

1965

J Bacteriol

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, AND H. R. HOMANN. Characteristics and intermediates of short-term C'402 incorporation during ribose oxidation by Hydrogenomonas facilis. J. Bacteriol. 89:839-847. 1965.-Ribose-grown cells of Hydrogenomonas facilis, which had been suspended in growth medium and were oxidizing ribose, were exposed to HC'403of high specific activity. The uptake was proportional to cell mass. Short-term uptake (less than 2 min) was completely inhibited by 10-3 M 2,4-dinitrophenol (DNP) or by <4 X 10-6 M m-chlorocarbonyl cyanide phenylhydrazone, and to the extent of 42% by 5 X 10-5 M DNP. The following observations were made in kinetic studies (8, 16, 35, 67, 96, and 181 sec) of fixation in the presence of ribose. Glutamate was extensively labeled in periods up to 3 min. It was one of the major early products, containing 30% of the label at 8 sec. The sugar phosphate fraction was not detectably labeled at 8 or 16 sec, but its C14-content increased rapidly to 27%o at 35 sec and then slowly decreased. Label in phosphoglycerate, phosphoenolpyruvate, and alanine did not appear until 35 sec, and did not exceed about 7, 2, and 3%, respectively, of the total extracted radioactivity. Adenosine triphosphate and adenosine diphosphate were heavily labeled after fixation in a pilot study for 125 sec. Although considerable radioactivity incorporated during the pilot study was intractable by the extraction procedure employed, virtually no C14 was found in the residue in poly-,B-hydroxybutyric acid. A large number of amino acids and organic acids and some organic phosphates were not detectably labeled in any of the experiments. Omission of ribose greatly diminished incorporation, particularly into glutamate.

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Section: Resultsmentioning

confidence: 99%

Characteristics and Intermediates of Short-Term C ¹⁴ O ₂ Incorporation During Ribose Oxidation by Hydrogenomonas facilis

McFadden

Homann

1965

J Bacteriol

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“…1 paper with the following solvent systems; n-butanol: acetic acid : water (4:1:1), s-butanol: t-butanol: 2-butanone: water (4:4:8:5) and propanol: water (4:1). For two-dimensional chromatography the first two systems were used (Ambe and Tappel, 1961).…”

Section: Preparation Of Enzymatic Digests Of Glutenmentioning

confidence: 99%

Studies on the mechanism of destruction of the toxic action of wheat gluten in coeliac disease by crude papain

Messer¹,

Anderson²,

Hubbard³

1964

Gut

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EDITORIAL SYNOPSIS The toxic action of wheat gluten on two patients was eliminated after predigestion of gluten by crude papain. This change is thought to be due to an enzyme which liberates ammonia from gluten. It is not thought that this particular mechanism operates in the normal intestinal cell but it is envisaged that one of the peptide bonds of the coeliac active constituent (possibly a N-glutaminyl peptide) is normally split by a specific intestinal peptidase which is defective in coeliac patients.Previous authors have shown that the harmful action of dietary wheat gluten in coeliac disease or non-tropical sprue is not eliminated by predigesting the gluten with either pancreatin or pepsin or trypsin

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“…The effect of moisture on the mobility of amino acids (448) and the effect of pH on double and triple zoning (410) have been investigated. Various new solvent systems have been proposed (23,205,447,775,1239), also paper chromatography at elevated temperatures (479, 1074) and the use of various dyes to serve as visible tracers of amino acids (713).…”

Section: Alcoholsmentioning

confidence: 99%

Chromatography

Heftmanń¹

1962

Anal. Chem.

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Improved separation of amino acids with a new solvent system for two-dimensional paper chromatography

Cited by 22 publications

References 0 publications

Characteristics and Intermediates of Short-Term C ¹⁴ O ₂ Incorporation During Ribose Oxidation by Hydrogenomonas facilis

Characteristics and Intermediates of Short-Term C ¹⁴ O ₂ Incorporation During Ribose Oxidation by Hydrogenomonas facilis

Studies on the mechanism of destruction of the toxic action of wheat gluten in coeliac disease by crude papain

Chromatography

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Improved separation of amino acids with a new solvent system for two-dimensional paper chromatography

Cited by 22 publications

References 0 publications

Characteristics and Intermediates of Short-Term C 14 O 2 Incorporation During Ribose Oxidation by Hydrogenomonas facilis

Characteristics and Intermediates of Short-Term C 14 O 2 Incorporation During Ribose Oxidation by Hydrogenomonas facilis

Studies on the mechanism of destruction of the toxic action of wheat gluten in coeliac disease by crude papain

Chromatography

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Characteristics and Intermediates of Short-Term C ¹⁴ O ₂ Incorporation During Ribose Oxidation by Hydrogenomonas facilis

Characteristics and Intermediates of Short-Term C ¹⁴ O ₂ Incorporation During Ribose Oxidation by Hydrogenomonas facilis