Improved Sensitivity in the Measurement of Residual Leukocytes in Platelet Products Using an Automated Leukocyte Counter

Abstract

The instrument provides a new method of enumerating residual leukocytes in platelet products with better sensitivity and easier procedure compared to the Nageotte method and will be useful for quality assurance purposes at blood centers.

A rapid and reliable flow cytometric routine method for counting leucocytes in leucocyte‐depleted platelet concentrates

“…The increasing use of leucocyte‐reduced platelet concentrates (PCs) has raised the need for a simple, stable and accurate routine method for counting low numbers of leucocytes and different methods have been suggested [1–6]. To determine a low number of residual leucocytes with a high precision, it is advantageous to use a reference population with a known number of particles, which allows the determination of leucocyte concentration.…”

Applying a novel statistical process control model to platelet quality monitoring

“…It is generally accepted that hemacytometer WBC counts are variable, and this technology may not be adequate with the degree of WBC reduction obtained using new technologies 18 . Statistical QC monitoring of WBC‐reduced blood components would also benefit from technologies that enumerate residual WBCs that are more sensitive and more reproducible than that of the Nageotte chamber 19 . Alternative measures of platelet quality that may correlate better with in vivo platelet viability could be incorporated into the process model 20 .…”