Improved segmented-scan spectral stitching for stable isotope resolved metabolomics (SIRM) by ultra-high-resolution Fourier transform mass spectrometry

Kang, Woo-Young; Thompson, P. Travis; El‐Amouri, Salim S.; Fan, Teresa W.‐M.; Lane, Andrew N.; Higashi, Richard M.

doi:10.1016/j.aca.2019.06.019

Cited by 5 publications

(5 citation statements)

References 37 publications

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“…Such SIM with longer transients increases sensitivity, RP, and mass accuracy, thereby improving the chances of polyisotopes and other minor constituents to be observed. This approach has previously been applied for the measurement of isotopic abundance and confirmation of formula assignments. − Here, a narrow SIM spectrum obtained in aFT mode ( m/z 378.06–405.26; measured RP = 2 200 000 at m/z 400; number of scans = 100) yielded 54 monoisotopic CHOP formulae, of which 20.4% ( n = 11) had their corresponding isotopic peak detected with up to two 13 C atoms. In comparison, in the broadband aFT spectrum (observed RP at m/z 400 = 1 200 000; number of scans = 1000), only two of the 55 monoisotopic CHOP species (3.6%) in the same m/z range had a corresponding 13 C 1–2 isotopic peak detected.…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Organophosphorus Compounds in Wildfire Smoke Using 21-T Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry

et al. 2022

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We present a detailed molecular characterization of organophosphorus compounds in ambient organic aerosol influenced by wildfire smoke. Biomass burning organic aerosol (BBOA) is an important source of phosphorus (P) to surface waters, where even a small imbalance in the P flux can lead to substantial effects on water quality, such as eutrophication, algal blooms, and oxygen depletion. We aimed to exploit the ultrahigh resolving power, mass accuracy, and sensitivity of Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS) to explore the molecular composition of an ambient BBOA sample collected downwind of Pacific Northwest wildfires. The 21-T FT-ICR MS yielded 10 533 distinct formulae, which included molecular species comprising C, H, O, and P with or without N, i.e., organophosphorus compounds that have long been quantified in wildfire smoke but have not yet been characterized at the molecular level. The lack of detailed molecular characterization of organophosphorus compounds in BBOA is primarily due to their inherently low concentrations in aerosols and poor ionization efficiency in complex mixtures. We demonstrate that the exceptional sensitivity of the 21-T FT-ICR MS allows qualitative analysis of a previously uncharacterized fraction of BBOA without its selective concentration from the organic matrix, exemplifying the need for ultrahigh-resolution tools for a more detailed and accurate molecular depiction of such complex mixtures.

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Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Organophosphorus Compounds in Wildfire Smoke Using 21-T Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry

et al. 2022

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“…Within the measured data set of 622 pixels generating [Ag + C 8 H 10 ] + , the ratio was 1.15 ± 0.41. Therefore, an isotopic component of ion signals can be missing in the mass spectra in the case of ions with intensities close to the noise level because software can falsely attribute low-intensity signals to noise . Note that we did not have any control of the internal data processing and did not modify it.…”

Section: Data Processingmentioning

confidence: 99%

Detection of Single Ag Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry

Žalud

Prysiazhnyi

Bednařík

et al. 2023

J. Am. Soc. Mass Spectrom.

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The detection of a single entity (molecule, cell, particle, etc.) was always a challenging subject. Here we demonstrate the detection of single Ag nanoparticles (NPs) using subatmospheric pressure laser desorption/ionization mass spectrometry (LDI MS). The sample preparation, measurement conditions, generated ions, and limiting experimental factors are discussed here. We detected from 84 to 95% of the deposited 80 nm Ag NPs. The presented LDI MS platform is an alternative to laser ablation inductively coupled plasma mass spectrometry for imaging distribution of individual NPs across the sample surface and has a great potential for multiplexed mapping of lowabundance biomarkers in tissues.

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“…The precursor ion filtering of the quadrupole, the quantification of the precursor in the orbitrap, and the parallel identification of fragmentation spectra in the LIT are combined. Researchers had developed SIM stitching techniques based on the LIT or QMS on high-resolution ion trap mass spectrometers such as ICR and Orbitrap (Southam et al, 2007;Kang et al, 2019). Multiple narrow SIM segments were collected together after acquisition to produce a wide range of spectra.…”

Section: Combination Of Orbitrap and Other Analyzersmentioning

confidence: 99%

Towards Higher Sensitivity of Mass Spectrometry: A Perspective From the Mass Analyzers

Li¹,

Chu²,

Tan³

et al. 2021

Front. Chem.

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Mass spectrometry (MS) is one of the most widely used analytical techniques in many fields. Recent developments in chemical and biological researches have drawn much attention to the measurement of substances with low abundances in samples. Continuous efforts have been made consequently to further improve the sensitivity of MS. Modifications on the mass analyzers of mass spectrometers offer a direct, universal and practical way to obtain higher sensitivity. This review provides a comprehensive overview of the latest developments in mass analyzers for the improvement of mass spectrometers’ sensitivity, including quadrupole, ion trap, time-of-flight (TOF) and Fourier transform ion cyclotron (FT-ICR), as well as different combinations of these mass analyzers. The advantages and limitations of different mass analyzers and their combinations are compared and discussed. This review provides guidance to the selection of suitable mass spectrometers in chemical and biological analytical applications. It is also beneficial to the development of novel mass spectrometers.

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Improved segmented-scan spectral stitching for stable isotope resolved metabolomics (SIRM) by ultra-high-resolution Fourier transform mass spectrometry

Cited by 5 publications

References 37 publications

Molecular Characterization of Organophosphorus Compounds in Wildfire Smoke Using 21-T Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry

Molecular Characterization of Organophosphorus Compounds in Wildfire Smoke Using 21-T Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry

Detection of Single Ag Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry

Towards Higher Sensitivity of Mass Spectrometry: A Perspective From the Mass Analyzers

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