Improved scaffold biocompatibility through anti-Fibronectin aptamer functionalization

Incorporating graphene oxide into biomimetic nano-microfibrous cellulose scaffolds for enhanced breast cancer cell behavior

“…Finally, the scaffolds with cells were coated with a layer of gold and observed by SEM. From the SEM images, the cell area (in lm 2 ) was defined as the area covered by the cell projected over the substrate (Collartdutilleul et al 2014;Galli et al 2016).…”

Achieving Controlled Biomolecule–Biomaterial Conjugation

“… 211 The effects of copper are even more deleterious for oligonucleotide sequences, with the copper-induced generation of reactive oxygen species (ROS) resulting in base modifications and backbone scission. 158 These problems are exacerbated by the need for high catalyst loadings to maintain conjugation efficiency, as a result of the high affinity of copper for both the triazole reaction product and many biologically relevant functional groups, effectively sequestering the active catalyst from the conjugation mixture. 200 These limitations can be partially mitigated by ligands able to strongly stabilize the active copper(I) catalyst or chelate azides to enhance reaction rates, 212 − 214 though copper induced damage to biomolecules is still problematic.…”

“…Indeed, many of the examples of biomaterial–oligonucleotide conjugation described in this review act as a means to subsequently bind and control the release of growth factors, cytokines, and ECM proteins. 76 , 158 , 352 , 353 , 355 , 356 , 482 DNA and RNA are inherently less stable than peptides and often more expensive to access synthetically. However, the ability to access long aptamer sequences is attractive, allowing the production of constructs with increased binding affinity.…”

Tailoring the Interface of Biomaterials to Design Effective Scaffolds

“…We obtained selective binding materials and in both the cases we observed great fibronectin adsorption from culturing medium and an enhanced proliferation, adhesion and migration of osteoblastic cells up to 7 days of culture. Interestingly, cell response was proportional to the amount of aptamers used for the functionalization, suggesting this novel approach as a viable alternative to tailor the surface bioactivity of a scaffold for TE applications [ 134 , 135 ].…”