Improved Salt Tolerance and Morphological Variation in Indica Rice (Oryza sativa L.) Transformed with a Catalase Gene from E. coli

Abstract

In an attempt to improve the salt tolerance of rice, we introduced katE, a catalase gene of Escherichia coli, into the indica rice cultivar Kasalath. Transformation was carried out using Agrobacterium tumefaciens strain EHA101 harboring a binary vector pIES6/Hm/katE which contains genes for catalase katE, hygromycine resistance gene HPT and kanamycin resistance gene NPTII in the T-DNA region. With the inclusion of acetosyringone, higher amount of transgenic cells and regenerated plants were obtained. Transformation was confirmed by PCR with katE and HPT primer. Transgenic plants at a very young stage (three -four days) were able to grow up to 15 days in 100 mM NaCl solution and seven days in 250 mM NaCl solution whereas control plants died within five days in 100 mM and seven days in 50 mM NaCl. Plants stressed for four weeks could survive for a long time and were able to flower. Different morphological characters varied in transgenic compared to control plants. Introduction of katE gene significantly improved the salt tolerance of the transgenic indica lines which could mature and set seed under stress.

Mature seed-derived callus of the model indica rice variety Kasalath is highly competent in Agrobacterium-mediated transformation

“…2 ). To date, transformation experiments using secondary callus derived from mature seeds and callus derived from immature embryos of Kasalath have been reported (Du et al 2009 ; Hiei and Komari 2008 ; Nishimura et al 2006 ; Prodhan et al 2008 ; Yamaya et al 2002 ). However, there are no reports of transformation using primary callus of Kasalath derived from mature seeds.…”

Genomic Analysis of a Highly Virulent NDM-1-Producing Escherichia coli ST162 Infecting a Pygmy Sperm Whale (Kogia breviceps) in South America

“…Halotolerance associated genes katE and nhaA, which encodes for catalase and Na + /H + antiporter cytoplasmic membrane, respectively, were in silico predicted (Rimon et al, 2007;Prodhan et al, 2008). Furthermore, genes conferring resistance to heavy metals [i.e., arsenic resistance (arsBCR), tellurite (tehAB)] and biocides [i.e., quaternary ammonium compounds (acrEF, emrK, mdtEFKN, mvrC, tolC, yjiO) and glyphosate (phnCDEFGHIJKLMNOP)] were also detected (Table 1).…”

De novo transcriptome analysis of halotolerant bacterium Staphylococcus sp. strain P-TSB-70 isolated from East coast of India: In search of salt stress tolerant genes

“…A number of researchers have investigated glycine betaine gene, trehalose gene, ectoine gene, sodium, and hydrogen antiporters from different bacteria viz. KatE, HPT and NPTII from Escherichia coli [64][65][66], BetSfrom Sinorhizobiummeliloti [13],ectABCfrom Chromohalobactersalexigens [16,67], ectABC from Bacillus halodurans [17], codA from Arthrobacter globiformis strain ATCC 8010 [68,69], a-aminoisobutyric acid (AIB) from Vibrio costicola [70], OpuC and OpuB from Listeria monocytogenes [71], ablA from MethanosarcinamazeiGo¨1 [72], otsA/ otsB, mpgS/mpgP from Thermus thermophilus [73], BetT from Pseudomonas syringae [74], proH, proJ and proA from Halobacillus halophilus [75], Mpgsmt-sdmt from Halophilic Methanogen Methanohalophilus portucalensis [76], Bacterial mannitol 1-phosphate dehydrogenase (mtlD) gene frpm mtlD producing bacteria [77].…”