Improved salinity tolerance of Medicago sativa and soil enzyme activities by PGPR

Tirry, Nabil; Kouchou, Aziza; Laghmari, Ghizlane; Lemjereb, Meryem; Hnadi, Hamza; Amrani, Khalid; Bahafid, Wifak; Ghachtouli, Naïma El

doi:10.1016/j.bcab.2021.101914

Cited by 40 publications

(21 citation statements)

References 69 publications

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“…Plant growth is inseparable from nutrient uptake by plants. According to Caldwell (2005) , soil enzyme activities reflect the soil community metabolic demands and available nutrients, since changes in soil nutrients are closely related to soil enzyme activities, which are of major significance for plant nutrition processes ( Tirry et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Plant Growth-Promoting Rhizobacteria Alleviate High Salinity Impact on the Halophyte Suaeda fruticosa by Modulating Antioxidant Defense and Soil Biological Activity

et al. 2022

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Plant growth-promoting rhizobacteria (PGPR) are considered as bio-ameliorators that confer better salt resistance to host plants while improving soil biological activity. Despite their importance, data about the likely synergisms between PGPR and halophytes in their native environments are scarce. The objective of this study was to assess the effect of PGPR (Glutamicibacter sp. and Pseudomonas sp.) inoculation on biomass, nutrient uptake, and antioxidant enzymes of Suaeda fruticosa, an obligate halophyte native in salt marshes and arid areas in Tunisia. Besides, the activity of rhizospheric soil enzyme activities upon plant inoculation was determined. Plants were grown in pots filled with soil and irrigated with 600 mM NaCl for 1 month. Inoculation (either with Pseudomonas sp. or Glutamicibacter sp.) resulted in significantly higher shoot dry weight and less accumulation of Na+ and Cl– in shoots of salt-treated plants. Glutamicibacter sp. inoculation significantly reduced malondialdehyde (MDA) concentration, while increasing the activity of antioxidant enzymes (superoxide dismutase; catalase; ascorbate peroxidase; and glutathione reductase) by up to 100%. This provides strong arguments in favor of a boosting effect of this strain on S. fruticosa challenged with high salinity. Pseudomonas sp. inoculation increased shoot K+ and Ca2+ content and lowered shoot MDA concentration. Regarding the soil biological activity, Pseudomonas sp. significantly enhanced the activities of three rhizospheric soil enzymes (urease, ß-glucosidase, and dehydrogenase) as compared to their respective non-inoculated saline treatment. Hence, Pseudomonas sp. could have a great potential to be used as bio-inoculants in order to improve plant growth and soil nutrient uptake under salt stress. Indole-3-acetic acid concentration in the soil increased in both bacterial treatments under saline conditions, especially with Glutamicibacter sp. (up to +214%). As a whole, Glutamicibacter sp. and Pseudomonas sp. strains are promising candidates as part of biological solutions aiming at the phytoremediation and reclamation of saline-degraded areas.

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Section: Discussionmentioning

confidence: 99%

Plant Growth-Promoting Rhizobacteria Alleviate High Salinity Impact on the Halophyte Suaeda fruticosa by Modulating Antioxidant Defense and Soil Biological Activity

et al. 2022

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“…The bacteria, Pseudomonas putida, Alcaligenes sp., Klebsiella sp., and Pseudomonas cedrina, used in this study were previously isolated from southern Morocco and characterized for their PGP traits and their resistance to salinity [9]. Pot experiments were designed in order to study the effect of the bacterial consortium on M. sativa (Alfalfa) under salt exposure.…”

Section: Methodsmentioning

confidence: 99%

Enhanced Salinity Tolerance of Medicago sativa, Roots AM Colonization and Soil Enzyme Activities by PGPR

Tirry

Ferioun

Kouchou

et al. 2022

The 2nd International Laayoune Forum on Biosaline Agriculture

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“…The selected isolate NT27 was characterized by a molecular identification approach using the universal primers fD1 (50 AGA GTT TGA TCC TGG CTC AG 30) and rD2 (50 ACG GCT ACC TTG TTA CGA CTT 30) [ 52 ]. Bacterial DNA extraction and fragment of rDNA amplification were realized as described by Tirry et al [ 53 ]. The sequences obtained were checked and extracted by Mega X (version 10.0.5).…”

Section: Methodsmentioning

confidence: 99%

Improved chromium tolerance of Medicago sativa by plant growth-promoting rhizobacteria (PGPR)

Tirry

Kouchou

Omari

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Soil pollution by heavy metals increases the bioavailability of metals like hexavalent chromium (Cr (VI)), subsequently limiting plant growth and reducing the efficiency of phytoremediation. Plant growth-promoting rhizobacteria (PGPR) have substantial potential to enhance plant growth as well as plant tolerance to metal stress. The aim of this research was to investigate Cr (VI) phytoremediation enhancement by PGPR. Results The results showed that the 27 rhizobacterial isolates studied were confirmed as Cr (VI)-resistant PGPR, by using classical biochemical tests (phosphate solubilization, nitrogen fixation, indole acetic acid, exopolysaccharides, hydrogen cyanide, siderophores, ammonia, cellulase, pectinase, and chitinase production) and showed variable levels of Cr (VI) resistance (300–600 mg/L). The best four selected Cr (VI)-resistant PGPR (NT15, NT19, NT20, and NT27) retained most of the PGP traits in the presence of 100–200 mg/L concentrations of Cr (VI). The inoculation of Medicago sativa with any of these four isolates improved the shoot and root dry weight. The NT27 isolate identified using 16S rDNA gene sequence analyses as a strain of Pseudomonas sp. was most effective in terms of plant growth promotion and stress level decrease. It increased shoot and root dry weights of M. sativa by 97.6 and 95.4%, respectively, in the presence of Cr (VI) when compared to non-inoculated control plants. It also greatly increased chlorophyll content and decreased the levels of stress markers, malondialdehyde, hydrogen peroxide, and proline. The results of the effect of Pseudomonas sp. on Cr content and bioaccumulation factor (BAF) of the shoots and roots of M. sativa plants showed the increase of plant biomass concomitantly with the increase of Cr root concentration in inoculated plants. This would lead to a higher potential of Cr (VI) phytostabilization. Conclusions This study demonstrates that the association M. sativa-Pseudomonas sp. may be an efficient biological system for the bioremediation of Cr (VI)-contaminated soils.

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Improved salinity tolerance of Medicago sativa and soil enzyme activities by PGPR

Cited by 40 publications

References 69 publications

Plant Growth-Promoting Rhizobacteria Alleviate High Salinity Impact on the Halophyte Suaeda fruticosa by Modulating Antioxidant Defense and Soil Biological Activity

Plant Growth-Promoting Rhizobacteria Alleviate High Salinity Impact on the Halophyte Suaeda fruticosa by Modulating Antioxidant Defense and Soil Biological Activity

Enhanced Salinity Tolerance of Medicago sativa, Roots AM Colonization and Soil Enzyme Activities by PGPR

Improved chromium tolerance of Medicago sativa by plant growth-promoting rhizobacteria (PGPR)

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