Improved RP‐HPLC and anion‐exchange chromatography methods for the determination of amino acids and carbohydrates in soil solutions

Meyer, Axel; Fischer, Holger; Kuzyakov, Yakov; Fischer, Klaus

doi:10.1002/jpln.200700235

Cited by 26 publications

(20 citation statements)

References 34 publications

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“…Amino sugar analysis based on Meyer et al (2008) was performed with a Dionex ion chromatography system consisting of an auto sampler AS 50, a gradient pump GP 40, and an electrochemical detector ED 40 equipped with a thin-layer-type amperometric cell. The detector cell integrated a gold working electrode and an Ag=AgCl reference electrode.…”

Section: Hpaec and Pulsed Amperometric Detection (Pad)mentioning

confidence: 99%

“…As described earlier (Bodé et al 2009), other concentrations of the NaOH eluent, other column temperatures and other flow rates decreased the selectivity of the amino sugar chromatography. Previous studies (Yu, Ding, and Mou 2003;Boschker et al 2008;Meyer et al 2008;Bodé et al 2009) have indicated that carboxylic acids like MurN do not elute with NaOH as the mobile phase because its carboxyl group has a very high affinity to the column material. Thus, an eluent with a higher ionic strength is needed for the elution of MurN.…”

Section: Hpaec-flmentioning

confidence: 99%

“…We used this method for testing whether the OPA derivatization had an effect on the measured amino sugar concentrations. For a comparison of the methods, it was necessary to use a similar mobile phase and, consequently, we used the method based on Meyer et al (2008). This method had to be modified to obtain a baseline separation of the three basic amino sugars at the beginning of the run.…”

Section: Hpaec-padmentioning

confidence: 99%

“…Other studies using anion exchange chromatography show similar results. Cataldi et al (1999) and Meyer et al (2008) determined amino sugars, sugars and uronic acids by using a gradient, whereas Kaiser and Benner (2000) as well as Bodé et al (2009) determined MurN in a separate chromatographic run.…”

Section: Comparison Of Rp-fl Hpaec-fl and Hpaec-padmentioning

confidence: 99%

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Comparison of HPLC Methods for the Determination of Amino Sugars in Soil Hydrolysates

Indorf¹,

Bodé²,

Boeckx³

et al. 2013

Analytical Letters

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A study on the suitability of chromatographic techniques such as high performance anion exchange chromatography (HPAEC) with fluorescence detection (FL) and pulsed amperometric detection (PAD) and reversed phase (RP) chromatography for the determination of galactosamine, glucosamine, mannosamine, and muramic acid in soil hydrolysates was carried out. The reversed phase fluorescence method was rapid, provided good validation parameters, and employed relatively inexpensive instrumentation. The HPAEC methods had slightly higher limits of quantification, 0.6-5.0 lmol L À1 (HPAEC-FL) and 1.0-10.0 lmol L À1 (HPAEC-PAD), compared to the reversed phase fluorescence method (0.5-5.0 lmol L À1 ). Various sample pretreatment methods and chromatographic methods were investigated and the advantages and disadvantages of the HPLC methods are discussed.

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Section: Hpaec and Pulsed Amperometric Detection (Pad)mentioning

confidence: 99%

Section: Hpaec-flmentioning

confidence: 99%

Section: Hpaec-padmentioning

confidence: 99%

Section: Comparison Of Rp-fl Hpaec-fl and Hpaec-padmentioning

confidence: 99%

See 2 more Smart Citations

Comparison of HPLC Methods for the Determination of Amino Sugars in Soil Hydrolysates

Indorf¹,

Bodé²,

Boeckx³

et al. 2013

Analytical Letters

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“…For AA and CH, the methods and chemicals presented in Meyer (2008) were used. Carboxylic acids were measured according to Neumann (2007) after isocratic separation on a reversed-phase C-18 column (GROM-SIL 120 ODS-5 ST, particle size 5 lm; length 250 mm, inner diameter 4.6 mm) with a Hypersil ODS guard column (20 mm, ID 4.6 mm; GROM, Herrenberg, Germany) in the ion-suppression mode (Neumann, 2006); 18 mM KH 2 PO 4 pH 2.1 was used as eluent with a flow rate of 0.5 mL min -1 and column temperature of 35°C.…”

Section: Hplc and Py-fims Measurementsmentioning

confidence: 99%

Rhizodeposition of maize: Short‐term carbon budget and composition

Fischer

Eckhardt

Meyer

et al. 2010

Z. Pflanzenernähr. Bodenk.

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The aim of this study was to assess differences in rhizodeposition quantity and composition from maize cropped on soil or on 1:1 (w/w) soil-sand mixture and distribution of recently assimilated C between roots, shoots, soil, soil solution, and CO 2 from root respiration. Maize was labeled in 14 CO 2 atmosphere followed by subsequent simultaneous leaching and air flushing from soil. 14 C was traced after 7.5 h in roots and shoots, soil, soil solution, and soil-borne CO 2 . Rhizodeposits in the leachate of the first 2 h after labeling were identified by high-pressure liquid chromatography (HPLC) and pyrolysis-field ionization mass spectrometry (Py-FIMS). Leachate from soil-sand contained more 14 C than from soil (0.6% vs. 0.4%) and more HPLC-detectable carboxylates (4.36 vs. 2.69 lM), especially acetate and lactate. This is either because of root response to lower nutrient concentrations in the soil-sand mixture or decreasing structural integrity of the root cells during the leaching process, or because carboxylates were more strongly sorbed to the soil compared to carbohydrates and amino acids. In contrast, Py-FIMS total ion intensity was more than 2 times higher in leachate from soil than from soil-sand, mainly due to signals from lignin monomers. HPLC-measured concentrations of total amino acids (1.33 lM [soil] vs. 1.03 lM [soil-sand]) and total carbohydrates (0.73 vs. 0.34 lM) and 14 CO 2 from soil agreed with this pattern. Higher leachate concentrations from soil than from soil-sand for HPLCmeasured carbohydrates and amino acids and for the sum of substances detected by Py-FIMS overcompensated the higher sorption in soil than in sand-soil. A parallel treatment with blow-out of the soil air but without leaching indicated that nearly all of the rhizodeposits in the treatment with leaching face decomposition to CO 2 . Simultaneous application of three methods-14 C-labeling and tracing, HPLC, and Py-FIMS-enabled us to present the budget of rhizodeposition ( 14 C) and to analyze individual carbohydrates, carboxylates, and amino acids (HPLC) and to scan all dissolved organic substances in soil solution (Py-FIMS) as dependent on nutrient status.

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Adsorption efficiency of a continuous trapping system and its use for the collection of root exudates from cucumber

Dong

Chu

et al. 2015

J. Plant Nutr. Soil Sci.

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Root exudates comprising soluble low-molecular-weight organic compounds (LMWOCs) play a crucial role in the rhizosphere processes. Therefore, accurate determination of the composition and quantity of these compounds is of importance. A continuous trapping system (CTS) with XAD-4 macroporous resin is widely used for collecting root exudates in hydroponics, and ion exchange resins are used in the separation of root exudates into neutral, acid and basic fractions. Here, we studied the adsorption equilibrium isotherms and dynamic adsorption properties of 27 standard LMWOCs on XAD-4 resin. The adsorption isotherms of most LMWOCs matched the Langmuir isotherm equations. Furthermore, we investigated the adsorption efficiency of the CTS for LMWOCs and the recovery ratios during fractionation by ion exchange resins. The adsorption capacities of the CTS for LMWOCs which are non-polar or having large non-polar moieties were higher. The recovery ratios of most LMWOCs were larger than 80% during fractionation. The overall recovery ratios of LMWOCs were ca. 10, 20, and 30% for sugars, organic acids, and amino acids, respectively. Using this collection method, we present the composition and quantity of root exudates of cucumber (Cumumis sativus L.) at four different growth stages. The major components of root exudates were similar to previous works, whereas the quantities were different. The various collection methods may be the main reason for these differences. Therefore, it is necessary to study the overall recovery ratios when the compositions of root exudates via different collection methods are compared.

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Improved RP‐HPLC and anion‐exchange chromatography methods for the determination of amino acids and carbohydrates in soil solutions

Cited by 26 publications

References 34 publications

Comparison of HPLC Methods for the Determination of Amino Sugars in Soil Hydrolysates

Comparison of HPLC Methods for the Determination of Amino Sugars in Soil Hydrolysates

Rhizodeposition of maize: Short‐term carbon budget and composition

Adsorption efficiency of a continuous trapping system and its use for the collection of root exudates from cucumber

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